GEN-MKT-18-7897-A
Jul 28, 2016 | Blogs, Life Science Research, Proteomics | 0 comments
SCIEX partners to improve depth of proteome coverageSCIEX and Pressure BioSciences address a major challenge for researchers performing complex sample preparation by marketing a complete solution to increase the depth, breadth, and reproducibility of protein extraction, digestion, and quantitation in all tissue types, especially challenging samples like tumors.
How it’s done!Pressure Cycling Technology (PCT) Sample Preparation Systems utilize controlled cycles of pressure to break apart the tissue samples. Scientists see faster and improved sample processing, and a higher quality of results. When combined with SWATH® Acquisition, high quality protein quantitation results can be obtained on 1000s of proteins in 100s of samples. Thus, combining SWATH® Acquisition with PCT sample preparation makes reproducible proteome research feasible across the enormous diversity of complex biological samples.
Who developed it and Why?PCT-HD was developed by PBI scientists and engineers in collaboration with Professor Ruedi Aebersold and Dr. Tiannan Guo at ETH Zurich. Drs. Aebersold and Guo combined PCT-HD sample preparation with SCIEX’s SWATH Mass Spectrometry in an effort to standardize the protocol for reproducible, comprehensive quantitation from complex samples. This unique protocol is capable of processing up to 16 samples in six hours1, much faster than current methods.
“By addressing the significant challenges inherent in complex sample preparation to reproducibly analyze thousands of proteins in hundreds of samples, PCT-SWATH accelerates proteomics research in biologically and clinically relevant contexts,” states Dr. Aebersold. “This should increase the productivity of biomarker research, potentially leading to significant improvements in healthcare, including personalized medicine.
Why PCT-HD with SWATH®Acquisition?
The Goal and SolutionOur goal is to industrialize proteomics by enabling efficient, reproducible and automated workflows specifically targeting analysis of small tissue samples for life science research. As the promise of precision medicine research continues to evolve, researchers will need powerful tools and application support to perform the Omics research that creates the scientific foundation of precision medicine. This solution from SCIEX and PBI is expected to significantly expand the footprint of MS-based quantitation workflows in clinical research settings worldwide.
Most recently, the Aebersold lab has combined the latest PCT technology with SWATH® acquisition to achieve a 40% increase in peptide quantitation over traditional methods.1 This means deeper proteome coverage with less sample input requirements.
To learn more about how the PCT sample prep workflow, our partners at PBI have recorded a short video.
Want to know more about available methods? Comment below!
References
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
CE‑SDS remains a cornerstone assay for characterizing fragmentation, aggregation, and product‑related impurities in therapeutic proteins. UV detection has been the long‑standing standard. However, it frequently struggles with baseline noise, limited sensitivity for minor fragments, and subjective integration.
At SCIEX, innovation doesn’t stop at instruments; it extends to how you interact with your LC-MS/MS or CE systems every day. That’s why we’re excited to introduce the SCIEX Now spring 2026 improvements: a set of meaningful enhancements shaped directly by your feedback.
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