GEN-MKT-18-7897-A
May 22, 2017 | Biopharma, Blogs | 0 comments
From small ions like phosphate, herbicide degradation to metabolites, oligosaccharides, peptides, and proteins. How is your lab analyzing polar molecules? The reason I ask is there is a saying, if you have a charged or polar molecule, look to capillary electrophoresis (CE) first. While liquid chromatography (LC) is an ideal front-end separation tool for many types of molecules, as the following poster points out, “From Small to Very Large, Orthogonal, Sensitive Polar Molecule Analysis by CESI-MS,” there are some situations that call for CE over LC analysis. For those of you that are not familiar with CESI-MS, it is the combining of CE separation with electrospray ionization, into one dynamic process, within the same device. View the Poster >
Complex Polar MoleculesLet me explain. Although effective, analysis by traditional LC-MS methods can present challenges that are addressed by the aqueous nature of capillary zone electrophoresis (CZE)-based separations. Therefore, if you have a choice of using LC-MS or CESI-MS when it comes to complex or polar molecules, the latter is ideal for your testing purposes since you can see and get high-resolution separation of PTMs missed by LC-MS methods. Such is the case for large molecules like intact monoclonal antibodies, and for small molecules, CESI can separate geometric isomers and isobaric compounds prior to mass spec analysis.
Want to see how CESI-MS plays out in the lab? View the Poster and get to know how molecules can be separated to differentiate their subtle structural changes. Collectively you’ll discover how the examples illustrate the simplicity of buffer systems that allow for the analysis of a wide range of highly relevant polar molecules by CESI-MS like phosphonate/phosphate for herbicide analysis to metabolites, oligosaccharides, and intact proteins.
What is more, you will also discover how you can improve the sensitivity, speed, and comprehensiveness of polar biomolecule analysis such as the separation of polar herbicide glyphosate and fungicide fosetyl aluminum. It’s not just polar ions the researchers put to the test either. Read about the results of anionic metabolites, glycans, and intact proteins too.
Find out how you can save time and resources using CESI-MS technology and read more about CE technology >
The Echo® MS+ system is a novel platform for Acoustic Ejection Mass Spectrometry (AEMS) and combines the speed of acoustic sampling with the selectivity of mass spectrometry. This platform has been designed for high throughput analysis of small and large molecules. The technology combines Acoustic Droplet Ejection (ADE), an Open Port Interface (OPI) and could be coupled with the SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system.
The Echo® MS+ system comprises of an open-port interface (OPI) and acoustic droplet ejection (ADE) module which could be coupled with a mass spectrometer. The mass spectrometer could either be a SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system. This non-liquid chromatography based; high-throughput screening platform enables rapid analysis of compounds at speeds of up to 1 sample/second.
The ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
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