GEN-MKT-18-7897-A
May 2, 2018 | Blogs, Forensic | 0 comments
Forensics depend on detection of even the smallest compounds to deliver results you can rely on. You need fast analysis methods that provide highly accurate data across a multitude of compounds and biomarkers, which enable you to uphold result integrity and get the truth from your samples.
Mass spectrometry has become fundamental to the evolution of forensic science. As instruments have become more sophisticated, law enforcement can now get results from the smallest samples of lingering traces of crime, in a manner that will stand up in court. However, the ever-increasing sensitivity and throughput requirements of forensic assays can pose method development challenges, even for the most powerful LC-MS/MS technology.
Getting the Right Answer is Crucial to Your Forensic InvestigationAs with many applications of quantitative analysis, forensic analytes can be difficult to accurately detect because of complex matrices or the presence of chemical interferences. Furthermore, compounds may be isobaric and consequentially challenging to separate with conventional separation technology.
Where LC-MS/MS sensitivity alone is not enough for complex assays that demand exceptionally selective quantitative and qualitative performance, Differential Ion Mobility Technology has proven to be a valuable addition.
SelexION® DMS Technology Will Breakthrough Sensitivity BarriersSelexION DMS Technology, coupled with QTRAP or TripleTOF LC-MS systems, can separate analytes of identical mass, reduce chemical noise, and improve quantitative accuracy, to rapidly deliver the forensic data you need. You can benefit from high throughput methods with little or no sample preparation and minimal chromatographic retention.
DMS separates ions based on the difference between their ion mobility’s in a high and low electric field in gases at or near atmospheric pressure. SelexION is placed in front of the inlet of the mass spectrometer, and the ionized molecules travel into the orthogonal geometry shaped DMS cell. Ions are separated on mobility scale due to differences in molecular size and shape, thereby providing highly selective analysis with minimal background interferences.
Advantages of the planar DMS geometry include:
SelexION offers a unique approach to separation that is unmatched by any other available method. Download the e-book to find out how we achieved LOD of 0.05 ng/mL and LOQ of 0.5 ng/mL using SelexION technology in the analysis of methyldienolone in urine, which were otherwise 2 and 5 ng/mL respectively without.
Find out about The Science Behind SelexION Differential Ion Mobility Technology and how SeleXION Addresses Your Biggest Analytical Challenges.
Finding the right information shouldn’t slow you down. Whether you’re troubleshooting your mass spec, learning something new, or optimizing performance, access to the right resources at the right moment makes all the difference.
As an analytical strategy, middle-down mass spectrometry (MS) workflows characterize biotherapeutic proteins by analyzing large, digested protein fragments or defined subunits, rather than fully intact proteins (top-down) or digested peptides (bottom-up). A middle-down strategy combines the strengths of top-down and bottom-up approaches by delivering high sequence coverage and structural specificity while maintaining relatively simple sample preparation. In practice, middle-down analysis enables accurate mass measurement, rapid sequence confirmation, and localization of key post-translational modifications (PTMs) on protein subunits that are directly relevant to product quality.
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
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