GEN-MKT-18-7897-A
Aug 15, 2018 | Blogs, Environmental / Industrial | 0 comments
Exposure to per- and polyfluoroalkyl substances (PFAS) residues could be dangerous. These chemicals have been linked to a variety of adverse health effects including liver damage, thyroid disease, decreased fertility, high cholesterol, obesity, hormone suppression, and cancer. In recognition of these potential risks, these water contaminants are making public headlines as well as becoming core topics for reports, and scientific studies.
What are PFAS’? PFAS are unique chemicals that repel both oil and water; this, among other properties, makes them useful in a variety of applications. Globally, there are more than 3000 products that either once used or still use PFAS3. Common examples include food packaging, stain-resistant fabrics and carpets, non-stick kitchenware, paints, adhesives, electronics, personal care products, and firefighting foams1. However, there is only a limited amount of information on the extent of PFAS usage as well as how much has accumulated in the environment and made their way into our drinking water systems2.
These substances are mixtures of humanmade fluoropolymers containing carbon-fluorine monomers1. Perfluorinated compounds are those in which all hydrogen atoms have been replaced by fluorine1,4. It is the stability of the C-F bonds which prevents these compounds from naturally decomposing in the environment and makes them highly resistant to acids, bases, oxidants, and heat. Whereas, the term ‘polyfluorinated’ applies to chemicals where not all the hydrogens on the carbons of the molecule are replaced by fluorine1,4. Per- and polyfluorinated substances represent a compound class of potentially hundreds of chemicals of varying chemical structures and behaviors.
But how is it getting into our drinking water? Although there is a lack of conclusive data to pinpoint the exact source, there are researchers who suggest that extensive exposure to PFAS in water is partly from firefighting foams and sprays used in training simulations by the military and airport workers.
So, what does this all mean for public health? Health effects are widely debated, though there are many studies that link PFAS exposure to a variety of health problems.5 For that reason, regulators such as the U.S. Environmental Protection Agency (EPA) set “Health Advisory” levels of PFOA and PFOS.
Drinking water supplies for 6 million U.S. residents exceed U.S. EPA’s lifetime health advisory (70 ng/L) for perfluorooctanoate (PFOA) and perfluorooctanesulfonate (PFOS)4. Additionally, the U.S. National Health and Nutrition Examination Survey reported PFASs to be present in 97% of individuals4,6. Exposure to PFAS’ has been linked to cancer, elevated cholesterol, obesity, immune suppression, and endocrine disruption in humans4. The overwhelming presence of PFAS’ in drinking water systems and humans has motivated both the regulatory and academic communities to look beyond the occurrence of the most notorious PFAS7.
What are the current regulations? The U.S. EPA advisory level is 70 ng/L of PFOA and PFOS combined in drinking water, yet some studies suggest this level may be 100-fold too high5. This new research has influenced some states, like Vermont, to impose or suggest lower acceptable limits. In 2016, Vermont adopted an advisory level legal limit of 20 ng/L for both PFOS and PFOA with other states like Minnesota, New Jersey, and Michigan following suit with their own levels. While water system operators take appropriate steps and suggested PFAS concentrations continue to decrease, there is still a need for sensitive and robust analytical methods to detect and measure PFAS in such systems.
Where can SCIEX help? To help you with your drinking water analysis, we have developed several protocols and tools to aid in PFAS quantification and screening in drinking water.
Fill out the form on the right to download an info kit, which will give you access to:
Download your info kit today and let’s start helping the public regain confidence in our drinking water.
References
*This content does not constitute legal advice. You should consult counsel to assure your procedures comply with applicable law and that it meets your needs.
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As an analytical strategy, middle-down mass spectrometry (MS) workflows characterize biotherapeutic proteins by analyzing large, digested protein fragments or defined subunits, rather than fully intact proteins (top-down) or digested peptides (bottom-up). A middle-down strategy combines the strengths of top-down and bottom-up approaches by delivering high sequence coverage and structural specificity while maintaining relatively simple sample preparation. In practice, middle-down analysis enables accurate mass measurement, rapid sequence confirmation, and localization of key post-translational modifications (PTMs) on protein subunits that are directly relevant to product quality.
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
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