How does the scoring and FDR analysis work for SWATH acquisition data processing? For proteomics by Christie Hunter | Sep 28, 2021 | Data processing, Life Science Research, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemSimilar extraction processes are used in both the SWATH acquisition microapp in PeakView software on the desktop and the Extractor app in OneOmics suite. After the fragment ions have been selected for each peptide, the extracted ion chromatograms are generated and the...
How are peptides and transitions selected for use in quantification, when importing from a ProteinPilot software group file – using SWATH acquisition microapp or Extractor in OneOmics suite? by Christie Hunter | Sep 28, 2021 | Data processing, Life Science Research, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemSimilar selection processes are used in both the SWATH acquisition microapp in PeakView software on the desktop and the Extractor app in OneOmics suite. During the protein grouping process in ProteinPilot software, there is an extensive analysis that is done on the...
What software do I need to process my SWATH acquisition data using the desktop or cloud solutions? by Christie Hunter | Sep 28, 2021 | Data processing, Life Science Research, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemDesktop solution: If you are processing your SWATH acquisition data using the SCIEX pipeline, there are a 3 software packages that work together seamlessly for processing. First, you would process your IDA data using ProteinPilot software to create an ion library. ...
What is the best way to build spectral ion libraries for use in processing of proteomics SWATH acquisition data? by Christie Hunter | Sep 28, 2021 | Data processing, Libraries, Life Science Research, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThe information needed for targeted data extraction in our current SWATH acquisition workflow is relatively simple. You need the parent ion m/z; you need the m/z and relative intensities of the major fragment ions that are produced during MS/MS; and you need to know...
What is variable window SWATH acquisition and why does it improve results quality? by Christie Hunter | Sep 28, 2021 | Data acquisition, Environmental / Industrial, Food / Beverage, Food and Beverage, Forensic, Life Science Research, Metabolomics, Proteomics, QTOF, SCIEX information, Toxicology, ZenoTOF 7600 systemTo continue to improve the quality of the SWATH acquisition data that one can achieve in complex matrices, we are working on ways that we can decrease the size of the Q1 window, while still maintaining full mass range coverage and optimal cycle times. One great way...