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Simultaneously Detect Different Types of Beer Microbes Using XP-PCR

Jul 17, 2015 | Blogs, Food / Beverage | 0 comments

Spoiler Alert – How to Save Time Testing for Bacteria in Beer?

Nothing ruins a batch of beer worse than bacteria, specifically Pediococcus and Lactobacillus. Too much of these hop resistant genes can get carried away causing spoilage and sour beer. It is why breweries put every batch of beer through a quality control check before it is sent out to the consumer.

However, sometimes bad batches make it out the door because wild yeasts can be too small to detect using conventional microbial techniques. To ensure accuracy and efficacy, however, the SCIEX food and beverage scientists are putting many a beer to the test using capillary electrophoresis (CE) multiplex PCR (XP-PCR).  This process has been proven to simultaneously identify six major genera of beer spoilage bacteria and yeast along with their potential to spoil beer by detecting five hop resistant markers within 24 hours of sampling.

Beer Testing Goes Molecular
The benefit to lab scientists is molecular testing delivers fast results and high-resolution separation. According to Handy Yowanto, Senior Product Manager, SCIEX Genetic Analysis Product, “If you want to skip the guesswork, molecular techniques are the answer. They detect different types of microbes in tank cleaning, brewing process and final product quality check (QC).” 

The Message Is This:
If you are using conventional microbial culturing techniques along with dye sequencing, you may be missing some bacteria. The reason being is these methods cannot detect hop resistant genes that allow unculturable or slow-growing microorganisms to flourish.  Don’t let your beer fall flat. Bacteria can be introduced at any stage of the brewing process, but you can learn how to attack the problem by detecting hop resistant genes.Read the Full Report >

Questions and answers to help improve your mycotoxin analysis

During a recent webinar I shared method details for mycotoxin analysis on the SCIEX 7500 system. In this blog i will share the Q&A for the submitted questions that we did not have chance to answer during the live webinar.

A 2-fold revolution: MS approaches for the bioanalysis of oligonucleotide therapeutics

In 1998, the US Food and Drug Administration (FDA) approved fomivirsen as the first therapeutic oligonucleotide therapeutic. This approval marked a revolution of mechanism of action discovered decades before finally coming to fruition. Since then, the landscape of chemical modifications of oligonucleotides, conjugations and formulations has evolved tremendously, contributing to improvements in stability, efficacy and safety. Today, more than a dozen antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) drugs are on the market, most of which are designated as orphan drugs for treating rare genetic diseases.

Is “right first time, every time” a pipedream for metabolite identification by LC-MS?

If we lived in an ideal world, it would be possible to unambiguously identify metabolites using a single analytical experiment. This analytical technique would need to be efficient and easily generate the information needed from a routine assay that is also robust, enabling confident decision-making during drug discovery.

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