GEN-MKT-18-7897-A
Jun 20, 2016 | Blogs, Environmental / Industrial, Food / Beverage | 0 comments
No other pesticide has courted more media attention and controversy in recent months than glyphosate, with governments and national agencies debating its use and health effects. The following links just show some of the media attention this organophosphorus compound has received:
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However irrespective of whether it poses a risk or not the analysis of glyphosate and other polar compounds in food and beverage products has been a challenge. Attaining the required sensitivity, dealing with complex matrices and sample prep can put a serious strain and demand on your laboratory. A common practice for the analysis of glyphosate and the other associated polar compounds such as AMPA, Glufosinate, and MMPA is to derivatize the samples during the preparation.
The derivatization method may be effective however there is a serious efficiency problem associated with it, the time it takes the analysts to prep and extract the sample. Derivatization can be a time-consuming procedure and can, therefore, affect a lab’s productivity, turnaround, and margins.
In a recent presentation, on the QTRAP® 6500+ System, we conducted an in-depth evaluation into the Quantitation of Underivatized Glyphosate and Other Polar Pesticides. This comprehensive study details the various techniques and LC conditions that we tested on a variety of matrices. In this study, we show how the SelexION® reduces interferences and can meet your required levels of sensitivity.
Finding the right information shouldn’t slow you down. Whether you’re troubleshooting your mass spec, learning something new, or optimizing performance, access to the right resources at the right moment makes all the difference.
As an analytical strategy, middle-down mass spectrometry (MS) workflows characterize biotherapeutic proteins by analyzing large, digested protein fragments or defined subunits, rather than fully intact proteins (top-down) or digested peptides (bottom-up). A middle-down strategy combines the strengths of top-down and bottom-up approaches by delivering high sequence coverage and structural specificity while maintaining relatively simple sample preparation. In practice, middle-down analysis enables accurate mass measurement, rapid sequence confirmation, and localization of key post-translational modifications (PTMs) on protein subunits that are directly relevant to product quality.
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
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