The Science Behind SelexION Differential Mobility Spectrometry Technology

Aug 22, 2017 | Blogs, Technology | 0 comments

Scientists and analysts across all fields of testing and research are increasingly challenged by complex samples requiring advanced analytical selectivity. And where LC-MS/MS sensitivity alone is not enough to meet the demands of modern day quantitative performance, Differential Ion Mobility Spectrometry (DMS) has proven to be a valuable addition.

Break Through the Selectivity Barrier of Your LC/MS-MS Separations
SCIEX offers the most innovative solution with its SelexION® DMS Technology. It is a small, planar mobility cell that is easy to install (in less than 2 minutes), easy-to-use and can significantly increase analytical separation power. No other ion mobility separation tool offers the reproducibility, robustness, and simplicity to deliver highly selective and sensitive quantitative and qualitative analyses, within a UHPLC time scale.

How does SelexION make this possible?
The SelexION DMS technology separates ions based on differences in mobility in two different regions of the field dependent mobility curve. Due to its small size, the system can operate with very short ion residence times with optimal performance when using chemical modifiers.  The DMS device can also be used in ‘transparent mode’ to allow for maximal workflow flexibility.

  • Mobility Separation – Two parallel flat plates define a mobility region, allowing nitrogen carrier gas to create forward directional flow between the ion source and the mass spec analyzer. Analytes are separated based on their respective size, shape, charge state and chemical interaction, before entering the mass spec. Unlike traditional ion mobility, ions are not separated in time as they traverse the cell. Instead, they are separated in trajectory based on the difference in their mobility between the high field and low field portions of the applied RF.
  • Modifier Separation – Polar modifiers in the transport region can lead to additional analyte separation and peak capacity. Chemical modifiers, such as isopropanol or acetonitrile, influence ion mobility based on their ability to cluster with analytes.


Discover high-throughput LNP-mRNA integrity profiling

Lipid-based nanoparticles (LNPs) are effective non-viral vectors for delivering messenger RNA (mRNA) products, most notably used for production of vaccines against the recent SARS-CoV-2 pandemic.

Eliminate chick culling with innovative technology

While it sometimes seems questionable whether humanity and modern technology can coexist, technological advances in science can help pave the way to more compassionate business practices.

Using wastewater monitoring to assess exposure to PFAS

Per-and polyfluorinated alkyl substances (PFAS) are known for their water- and grease-resistant properties, which make them useful in many everyday items. In fact, a study from 2020 estimated over 200 “use categories” covering more than 1,400 individual PFAS compounds in commercial products—they are truly all around us. Due to their extensive presence and potentially harmful effects (these effects are still mostly uncertain), exposure to PFAS is a growing concern. Humans and wildlife have been exposed to these chemicals through a variety of routes, including food packaging, drinking water and cleaning products.

Posted by


Submit a Comment