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Adding the PepCalMix to your SWATH acquisition ion Library as a Retention Time Calibration Protein

Adding the PepCalMix to your SWATH acquisition ion Library as a Retention Time Calibration Protein

by | Sep 28, 2021 | Data processing, Life Science Research, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 system

The most important consideration in adding a new RT calibration protein to your library is making sure you are getting the retention time for both into the same retention time or chromatographic frame. Below is a list of steps that walks you through how to do this....
Adding the PepCalMix to your SWATH acquisition ion Library as a Retention Time Calibration Protein

How does the scoring and FDR analysis work for SWATH acquisition data processing? For proteomics

by | Sep 28, 2021 | Data processing, Life Science Research, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 system

Similar extraction processes are used in both the SWATH acquisition microapp in PeakView software on the desktop and the Extractor app in OneOmics suite. After the fragment ions have been selected for each peptide, the extracted ion chromatograms are generated and the...
Adding the PepCalMix to your SWATH acquisition ion Library as a Retention Time Calibration Protein

How are peptides and transitions selected for use in quantification, when importing from a ProteinPilot software group file – using SWATH acquisition microapp or Extractor in OneOmics suite?

by | Sep 28, 2021 | Data processing, Life Science Research, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 system

Similar selection processes are used in both the SWATH acquisition microapp in PeakView software on the desktop and the Extractor app in OneOmics suite. During the protein grouping process in ProteinPilot software, there is an extensive analysis that is done on the...
Adding the PepCalMix to your SWATH acquisition ion Library as a Retention Time Calibration Protein

What software do I need to process my SWATH acquisition data using the desktop or cloud solutions?

by | Sep 28, 2021 | Data processing, Life Science Research, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 system

Desktop solution: If you are processing your SWATH acquisition data using the SCIEX pipeline, there are a 3 software packages that work together seamlessly for processing.  First, you would process your IDA data using ProteinPilot software to create an ion library. ...

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