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What is the best way to build spectral ion libraries for use in processing of proteomics SWATH acquisition data?

What is the best way to build spectral ion libraries for use in processing of proteomics SWATH acquisition data?

by | Sep 28, 2021 | Data processing, Libraries, Life Science Research, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 system

The information needed for targeted data extraction in our current SWATH acquisition workflow is relatively simple.  You need the parent ion m/z; you need the m/z and relative intensities of the major fragment ions that are produced during MS/MS; and you need to know...
What is the best way to build spectral ion libraries for use in processing of proteomics SWATH acquisition data?

What is variable window SWATH acquisition and why does it improve results quality?

by | Sep 28, 2021 | Data acquisition, Environmental / Industrial, Food / Beverage, Food and Beverage, Forensic, Life Science Research, Metabolomics, Proteomics, QTOF, SCIEX information, Toxicology, ZenoTOF 7600 system

To continue to improve the quality of the SWATH acquisition data that one can achieve in complex matrices, we are working on ways that we can decrease the size of the Q1 window, while still maintaining full mass range coverage and optimal cycle times.  One great way...
What is the best way to build spectral ion libraries for use in processing of proteomics SWATH acquisition data?

What is SWATH acquisition and what are the critical acquisition attributes?

by | Sep 28, 2021 | Data acquisition, Food / Beverage, Food and Beverage, Forensic, Fundamentals, Life Science Research, Metabolomics, OneOmics, Pharma, Proteomics, QTOF, SCIEX information, Toxicology, ZenoTOF 7600 system

In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition,...

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