by Christie Hunter | Sep 20, 2021 | Data processing, Life Science Research, Optimizing Performance, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 system
The FASTA file required for use with the SWATH acquisition performance kit installs with ProteinPilot software or is available within OneOmics suite. Download this SWATH acquisition ion library for use with the SWATH acquisition performance kit Human2D_SOP_PepCalMix_...
by Christie Hunter | Sep 20, 2021 | Data acquisition, Development, Ion sources, LC, Life Science Research, Metabolomics, Pharma, Proteomics, SCIEX information
To set up a SCIEX high flow source for microflow LC (Turbo V ion source, DuoSpray source or IonDrive Turbo V ion source), first you must replace the wider bore electrodes with more narrow bore hybrid electrodes. Note with the OptiFlow Turbo V ion sources, there are...
by Christie Hunter | Sep 20, 2021 | Data acquisition, LC, Life Science Research, Optimizing Performance, Proteomics, SCIEX information
Symptom: LC-MS peptide peak are is smaller than expected in PepCalMix Test Test: Use PepCalMix Infusion tests to determine if it is a problem with LC or MS Solution: First perform the SCIEX recommended performance tests using infusion on your MS system. If these...
by Christie Hunter | Sep 20, 2021 | Data acquisition, LC, Life Science Research, Optimizing Performance, Proteomics, SCIEX information
Symptom: The observed retention times of the peptides eluting are compressed, are not as spread out as expected, suggesting the elution gradient is compressed Test: Use PepCalMix LC-MS test to check the peptide elution time and spread Solution: Make sure you are...
by Christie Hunter | Sep 20, 2021 | Data acquisition, LC, Life Science Research, Optimizing Performance, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 system
Symptom: Early eluting peptides are missing (based on monitoring PepCalMix LC-MS test or observing a change in the shape of the proteome TIC). Test: Use PepCalMix LC-MS test to monitor binding of early eluting peptides. There are a number of very hydrophilic peptides...
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