A rising star in food allergen research: proteomics of shellfish allergen by SCIEX Community | 0 CommentsIt’s important to know what you’re eating, especially if you suffer from a food allergy. About 220 million people worldwide live with a food allergy.1 These numbers, along with the complexity and severity of conditions, continue to rise. In America, there are about 32 million food allergy sufferers—5.6 million of those are children under the age of 18.2.2 That’s 1 out of every 13 children, or about 2 in every classroom. From a financial perspective, the cost of food allergy childcare for US families is up to $25 billion
A Smart Way to Profit from the Wealth of Biobanks by SCIEX Community | 0 Comments Microflow LC with SWATH® Acquisition for Digitizing Biobanks What if you could access thousands of high-quality samples for your research? What if these samples were well-annotated biological specimens? And what if they were carefully segmented into just the...
“Bottoms Up” Proteomics by Tom Knapman | 0 Comments Ahhhh beer. It's a ubiquitous drink found in over 90% of all countries around the world. Since the dawn of civilization, man has celebrated with beer where it can make even the most introverted person suddenly dance a little jig or belt out a top 40 song. But other...
Metabolomics Studies Benefit Biomedical Research by SCIEX Community | 0 CommentsProfessor Dr. Thomas Hankemeier, Head of the Division of Systems Biomedicine and Pharmacology, LACDRLACDR is a center of excellence for multidisciplinary research into drug discovery and development, with a strong focus on metabolomics. As part of its research...
A rising star in food allergen research: proteomics of shellfish allergen by SCIEX Community | Blogs, Food / Beverage, Life Science Research, ProteomicsIt’s important to know what you’re eating, especially if you suffer from a food allergy. About 220 million people worldwide live with a food allergy.1 These numbers, along with the complexity and severity of conditions, continue to rise. In America, there are about 32 million food allergy sufferers—5.6 million of those are children under the age of 18.2.2 That’s 1 out of every 13 children, or about 2 in every classroom. From a financial perspective, the cost of food allergy childcare for US families is up to $25 billion
A Smart Way to Profit from the Wealth of Biobanks by SCIEX Community | Blogs, Life Science Research, Proteomics Microflow LC with SWATH® Acquisition for Digitizing Biobanks What if you could access thousands of high-quality samples for your research? What if these samples were well-annotated biological specimens? And what if they were carefully segmented into just the...
“Bottoms Up” Proteomics by Tom Knapman | Blogs, Life Science Research, Proteomics Ahhhh beer. It's a ubiquitous drink found in over 90% of all countries around the world. Since the dawn of civilization, man has celebrated with beer where it can make even the most introverted person suddenly dance a little jig or belt out a top 40 song. But other...
Metabolomics Studies Benefit Biomedical Research by SCIEX Community | Blogs, Life Science Research, MetabolomicsProfessor Dr. Thomas Hankemeier, Head of the Division of Systems Biomedicine and Pharmacology, LACDRLACDR is a center of excellence for multidisciplinary research into drug discovery and development, with a strong focus on metabolomics. As part of its research...
Multi-Laboratory Study Highlights the Quantitative Reproducibility of SWATH Acquisition (Nature Communications Paper) by Christie Hunter | Blogs, Life Science Research, ProteomicsReproducibility is one of the key tenets of the scientific method. But in a recent survey published in Nature, more than 70% of researchers were not able to reproduce another scientist’s experiments, and more than half could not reproduce their own experiments1. While the reasons for this are many, at least some of them stem from issues inherent in data collection.
SCIEX Lands HUPO Science and Technology Award by Tom Knapman | Blogs, Life Science Research, ProteomicsWe are pleased to congratulate its research scientists Stephen Tate and Ron Bonner (retired) for being awarded this year’s Science and Technology award at HUPO 2017 in Dublin Ireland. The Science and Technology Award at HUPO recognizes an individual or team who were key in the commercialization of a technology, product, or procedure that advances proteomics research
Take on New Analytical Challenges with the TripleTOF 6600 Accurate Mass System by SCIEX Community | Blogs, TechnologyStay ahead of the curve by using the most advanced and versatile high-resolution accurate mass technology. The TripleTOF® 6600 accurate mass QTOF system can help you to analyze your samples faster, without compromising between speed, resolution, and sensitivity. Explore the technological advances that give this system the performance edge.
Happy Birthday to SWATH Acquisition! 5 Years of Innovation by Christie Hunter | Blogs, Life Science Research, ProteomicsWith its introduction at the HUPO World Congress in 2010 in Sydney Australia by Ruedi Aebersold, SWATH® Acquisition instantly intrigued scientists around the world. Here was a new technique with the potential to revolutionize the way proteomics studies were performed! Based on a data independent acquisition strategy using a SCIEX TripleTOF® 5600 system, SWATH was able to consistently identify and quantify at least as many peptides and proteins as other far more mature proteomics strategies on the market, but with quantitative accuracy and reproducibility rivaling gold standard MRM experiments! This solution was made broadly available to researchers with a full launch of SWATH Acquisition in the Analyst® TF 1.6 Software on the TripleTOF 5600+ System at ASMS 2012 in Vancouver (A Mine of Quantitative Proteomic Information. Prof Dr. Ruedi Aebersold, Head of the Department of Biology, ETH Zurich).
5 Tips for Calibrating a QTOF Mass Spectrometer by SCIEX Community | Blogs, Life Science Research, Proteomics, TechnologyDo you have questions about your mass spec? How about a workflow? Our community members are involved in active discussions and receive expert answers from customers like you, SCIEX scientists, and support specialists every week. One recent topic concerned the automatic calibration on TripleTOF® systems as answered by Dr. Christie Hunter whose focus is developing and testing innovative MS workflows for omics research through working collaboratively with the instrument, chemistry, and software research groups.
A Mine of Quantitative Proteomic Information by SCIEX Community | Blogs, Life Science Research, ProteomicsThe Aebersold group at ETH Zurich focuses on proteomics research, including the development of techniques to study the proteome as an integrated entity. In collaboration with SCIEX, the group established SWATH® Acquisition mass spectrometry, a data-independent acquisition (DIA) method capable of fragmenting multiple peptide species concurrently. The resulting comprehensive data set can be retrospectively re-mined, enabling maximum benefit to be derived from any study.
SWATH Acquisition – Master of All Trades by SCIEX Community | Blogs, TechnologySWATH® Acquisition is an innovative strategy for acquiring data on a TripleTOF® mass spectrometer. In a previous blog, we learned how SWATH works. Now let’s learn what it can do for different applications:
Data Independent Acquisition Mass Spectrometry with the Power of SWATH by SCIEX Community | Blogs, Life Science Research, Metabolomics, ProteomicsThere are many different methods in use today to acquire data on a mass spectrometer, but few have generated as much buzz in recent years as SWATH technology. First reported 5 years ago by Ruedi Aebersold and his group1, SWATH® Acquisition on a TripleTOF® instrument has rapidly become one of the premier acquisition strategies for identification and quantitation of complex samples. But what exactly is SWATH and why is it so powerful? In order to answer these questions, let’s first take a step back and look at the larger picture.
Stoller Biomarker Discovery Centre, Addressing Some of the Biggest Issues in Medicine by SCIEX Community | Blogs, Life Science Research, ProteomicsThe Stoller Biomarker Discovery Center, developed in partnership with SCIEX, was created to develop new omics technologies for biomarker research to understand the root cause of diseases such as cancer, cardiovascular disease, and autoimmune diseases. We initially announced our collaboration with the University of Manchester back in October 2015.
The History of Isotopic Labels for Quantitative Proteomics by Subhasish.Purkayastha@sciex.com | Blogs, Life Science Research, ProteomicsProteomics has become a vital tool for biological scientists performing research on the healthy and diseased states of living things. It involves the large scale and systematic analysis of all proteins within a given cell, tissue, or organism. Because proteins are regulated by many different internal and external stimuli, the proteome is dynamic and quantities of proteins can change from one state to the next. Therefore, in order to be of the highest utility, proteomics experiments need to both identify and quantify proteins so that comparative studies can be done, such as between healthy cells and tumor cells, or the comparison of different treatment regimens.
Improved complex sample processing for higher quality of results, reproducibility and depth of proteomic analysis by ken.hamill@sciex.com | Blogs, Life Science Research, ProteomicsSCIEX partners to improve depth of proteome coverage SCIEX and Pressure BioSciences address a major challenge for researchers performing complex sample preparation by marketing a complete solution to increase the depth, breadth, and reproducibility of protein extraction, digestion, and quantitation in all tissue types, especially challenging samples like tumors.
Mass Spec Strategies for Plant Metabolomics by Baljit Ubhi | Blogs, Life Science Research, MetabolomicsThe metabolome is the set of all low molecular weight compounds (typically less than ~2000 Da) that are present within an organism, tissue, or cell. Within the mass spectrometry (MS) community, the systematic and comprehensive analysis of the metabolome – i.e., metabolomics – has become an important and increasingly popular area of research. Because the metabolome is dynamic and ever-changing, it provides a snapshot into the state of the organism at the time of measurement. Compounds may appear or disappear, or quantities may change, depending on environmental factors and internal processes. Thus, the metabolome can be thought of as a unique chemical signature providing important information about the health and inner-workings of a biological system at any given moment in time.
Industrialize Your Quantitative Proteomics with the OneOmics Project by Christie Hunter | Blogs, Life Science Research, ProteomicsFor many labs, the days are long gone when it was acceptable to run only a few samples a week for your quantitative proteomics projects. The pressure for faster turn-around times, to support larger cohort studies, to sustain multiple research directions, and to transition from a purely unbiased discovery mode to verifying something truly unique and interesting, all demand a faster pace. Many labs are now being asked to analyze a hundred samples a week or more. In part 1 of this blog series, we saw how moving to a microflow SWATH workflow can dramatically increase your throughput with little compromise on overall results. In this part, we’ll address what to do with all of this data because it’s just no good if all we’ve done is move the bottleneck downstream.
Industrialize Your Quantitative Proteomics with Microflow Analysis by Christie Hunter | Blogs, Life Science Research, ProteomicsMany groups around the world are now using SWATH Acquisition on TripleTOF Systems for both quantitative proteomics experiments and biomarker research. The SWATH acquisition technique on a TripleTOF® 6600 system provides state-of-the-art quantitative proteomics analysis with unrivaled proteome coverage. With this workflow, researchers can routinely quantify 1000s of proteins per run, reproducibly, and with high data completeness – all with the quantitative accuracy approaching that of a gold standard MRM approach.
Bottom-Up Proteomics: A Discussion with Christie Hunter by Christie Hunter | Blogs, Life Science Research, ProteomicsBiocompare recently featured an article on Bottom-Up Proteomics. I had a chance to follow up with Christie Hunter and expand on some of the questions featured in the article:
Taking on Precision Medicine with Industrialized Proteomics by Neil Walsh | Blogs, Life Science Research, OneOmics, ProteomicsWhat if we could deliver the right treatment at the right time, to the right person to better, more effectively treat complex disease? This is the promise of precision medicine, to be able to approach complex disease treatment and prevention by taking into account individual variability in genes, environment, and lifestyle for each person.
Use a Bigger SWATH Library and Get More Protein Coverage From Your Sample by Christie Hunter | Blogs, Life Science ResearchIf you have a TripleTOF® System and you’re using SWATH Acquisition for your quantitative proteomics experiments, you’ve made the right choice. SWATH is a data independent workflow that enables data to be acquired for every detectable analyte in a complex sample. You create a digital map of the sample that can be mined for new information any time new biological insights are hypothesized. It’s all there!
Gain New Insights Into Human Spermatogenesis with this “Click & Easy” OneOmics Workflow by Fabienne Le Floch | Blogs, Life Science Research, ProteomicsA recent webcast by Charles Pineau, Director of Protim, IRSET, Rennes, France, demonstrates how you can use the OneOmics™ Platform as a "Click & Easy" workflow for integrating next-generation proteomics (NGP) data with next-generation sequencing (NGS) data. Dr....
Discover How SWATH Acquisition Technology is Being Used in HIV-1 Research by sara.feltesse@sciex.com | Blogs, Life Science Research, ProteomicsSWATH® Acquisition: On the Forefront of HIV-1 Research World AIDS Day is held on the 1st December each year and is an opportunity for people worldwide to unite in the fight against HIV, show their support for people living with HIV, and to commemorate people who have...
Analyze Large Omic Data Sets with Cloud Computing and See What More You Can Accomplish by SCIEX Community | Blogs, Life Science Research, ProteomicsOur scientific world is expanding. With each passing day, new discoveries are made, old discoveries are verified, and more data are generated. Large data sets are now the norm for omics experiments such as genomics, transcriptomics, proteomics, and metabolomics....
What You Need to Know About the OneOmics Project – A Discussion with Mark Cafazzo by SCIEX Community | Blogs, Life Science ResearchI recently had the opportunity to sit down with Mark Cafazzo, Global Proteomics Market Manager, SCIEX, to discuss the SCIEX OneOmics™ Project: Becky: I’ve heard about the OneOmics Project. Can you share more about it?
Using Mass Spectrometry to Screen Hundreds of Known and Unknown Metabolites by Baljit Ubhi | Blogs, Life Science Research, MetabolomicsIn the field of metabolomics, you typically choose to identify and characterize as many compounds as possible in an unbiased fashion, or screen for a specific set of compounds that are biologically relevant to your research. The beauty of the TripleTOF® System is that...
Using Mass Spectrometry for Down Syndrome Metabolomic Research by SCIEX Community | Blogs, Life Science Research, MetabolomicsLast week we launched a really interesting research project with Jeff Miller, Senior SCIEX Scientist, using a SCIEX QTRAP® 6500 mass spectrometer. Our plan was to test whether acetaminophen is metabolized differently in people with Down Syndrome (our younger brother William has Down Syndrome).
MultiOmics Research For the Study of Placental Development by Fabienne Le Floch | Blogs, Life Science Research, ProteomicsA recent study by Katy Williams (UCSF), Christie Hunter (SCIEX), and Andrew Olsen (Advaita) used the iPathwayGuide within the OneOmics cloud computing environment to help understand how placental development can go awry during certain pregnancy complications such as pre-eclampsia.