Rapid Characterization of Biologics using CESI-MS

Jul 7, 2016 | Biopharma, Blogs | 0 comments

Today, 30 monoclonal antibodies (mAbs), have been approved for the treatment of certain cancers, autoimmune and infectious diseases. Even more are in development, and perhaps you and your team of scientists are working on one now.  Keeping pace with fast development timelines while performing comprehensive characterization of biologic candidates can be challenging. However, more and more, scientists are tackling these challenges with new techniques to speed and simplify their characterization workflows. Read more in the application note, “Rapid Characterization of Biologics using a CESI 8000 – SCIEX TripleTOF® System,” found in the Biologics Analytical Characterization Compendium, which highlights how CESI separation coupled with high-resolution mass spectrometry can provide a comprehensive characterization of biotherapeutics.

Download the Compendium to learn more >

Overview
Capillary electrophoresis (CE) integrated with electrospray ionization (ESI) creates a single dynamic process, called CESI. CESI coupled with the Information Dependent Acquisition (IDA) mode of a TripleTOF® 5600+ or 6600 systems can enable you to:

  • Use a single enzyme, single digestion protocol, followed by a single separation step to rapidly attain 100% primary sequence coverage for both the heavy and light chains of your mAb.
  • Identify important glycoforms, including those in low abundance.
  • Characterize amino acid modifications, including oxidation, deamidation, and lysine heterogeneity.

Make the shift to better biological characterization. Get to know the detailed analysis of experiments like this and much more including intact mass analysis, purity and heterogeneity determination, glycan analysis, biosimilar comparability, and host cell protein detection. With better information, you can make better decisions. Let the Biologics Characterization Compendium help guide you.

See the results in the full article, pages 56-65, by downloading the Biologics Analytical Characterization Compendium.

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