GEN-MKT-18-7897-A
Aug 22, 2016 | Blogs, Food / Beverage | 0 comments
Quantitating antibiotics and insecticides in poultry is serious business. Overuse can lead to antibiotic resistance while insecticide residuals can cause harmful side effects in humans. In the United States, for example, the Federal Drug Administration (FDA), has offered up a plan to limit common antibiotics in feed, which are used to encourage growth. However, this is a voluntary plan, and as the following application note, “Quantitation of Antibiotics and Insecticides in Poultry Feed using LC-MS/MS,” points out, antibiotics have been shown to accumulate in poultry feathers, which are in turn used for nutritional elements in the feed. Therefore, as the government agency works on getting suppliers on board with the new plan, scientists are working on a testing method of their own which detects nine antibiotics and four insecticides in poultry feed including:
Perhaps you have seen this study, but just in case you missed it, we have included the full report in the Food & Beverage Compendium, which is now available for download.Download the Food & Bev Compendium >
Application Note at a Glance: To give you more insight into this application note and others like it in the compendium, consider how poultry feed contains many nutritional ingredients. Researchers had to design a method that included a hefty extraction method and clean-up efforts. As a result, they designed a single method to quantify a wide selection of antibiotics and insecticides in poultry feed using a QTRAP® 5500 for detection. Want to see how the extraction/sample prep was carried out? These experimental conditions along with separation and MS/MS detection are included within the compendium (pages 121 to 125).
The Food & Beverage Compendium is full of similar studies on topics including:
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As an analytical strategy, middle-down mass spectrometry (MS) workflows characterize biotherapeutic proteins by analyzing large, digested protein fragments or defined subunits, rather than fully intact proteins (top-down) or digested peptides (bottom-up). A middle-down strategy combines the strengths of top-down and bottom-up approaches by delivering high sequence coverage and structural specificity while maintaining relatively simple sample preparation. In practice, middle-down analysis enables accurate mass measurement, rapid sequence confirmation, and localization of key post-translational modifications (PTMs) on protein subunits that are directly relevant to product quality.
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
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