GEN-MKT-18-7897-A
Apr 17, 2018 | Biopharma, Blogs, Pharma | 0 comments
High selectivity is a key component of successful quantitative bioanalysis. As a bioanalyst, we need consistently accurate and robust quantitation of small molecule therapeutics and metabolites. Challenged by complicated matrix interferences, high baseline signal, and lack of isomer resolution, we need to embrace innovative strategies to increase the sensitivity and selectivity of our assays. If our ‘go-to’ is the development of complex HPLC conditions or modifying sample preparation procedures, we will find ourselves bogged down with time-consuming methods that reduce sample throughput. So, what’s the answer?
Imagine a new dimension in bioanalytical selectivity that you never thought possible. One that enables robust and reproducible bioanalytical methods, and meets high throughput requirements. One that reduces background noise, eliminates interferences and separates highly similar compounds, without having to resort to complex HPLC conditions or sample workup procedures.
Well this could very much be your reality.
Significant advances have been made in increasing MS/MS selectivity beyond the gold standard MRM. For example, MRM3 on the QTRAP® systems adds additional selectivity by increasing the number of fragmentation steps by the mass analyzer. Ion mobility presents another attractive option by introducing an orthogonal level of separation after sample ionization, followed by traditional m/z detection.
Although ion mobility techniques have been used extensively for qualitative applications, they have traditionally lacked the required ruggedness and speed required for quantitative bioanalysis. Not anymore, and hereby the end of the history lesson!
SelexION® DMS Technology provides an orthogonal level of separation. It is the first differential ion mobility separation technology to combine sensitivity and selectivity with unmatched reproducibility and robustness. It delivers enhanced analytical separations on demand for isobaric species and co-eluting contaminants, all in an elegantly simple package.
Along with the QTRAP 6500+ or 5500 systems, you get the power of differential ion mobility separation, enabled by multiple new innovations in ion mobility:
Do you want to know more? We thought you might, so we have developed some tech notes to support you in your quest to achieve high performing small molecule therapeutics and metabolite quantitation workflows in drug discovery and development:
If you are challenged by complex small molecule therapeutics and metabolite quantitation requiring advanced analytical selectivity, look no further. Download the eBook and get access to these technical papers, and so much more. Find out about The Science Behind SelexION Differential Ion Mobility Technology and how SeleXION Addresses Your Biggest Analytical Challenges.Download the eBook >
Trifluoroacetic acid (TFA) is emerging as one of the most concerning ultrashort-chain PFAS in Europe’s food supply – particularly in cereals, a staple consumed daily by millions. A report from PAN Europe reveals a widespread and largely unmonitored contamination trend that raises serious questions about food safety, regulatory blind spots, and future monitoring strategies.
PFAS analysis is complex, but expert guidance doesn’t have to be. In this episode of our ‘Ask the PFAS expert series’, we’re joined by Michael Scherer, Application Lead for Food and Environmental, to answer the most pressing questions in PFAS analysis. From why LC-MS/MS systems are the gold standard for analyzing diverse PFAS compounds, to which EU methods deliver reliable results for drinking water, and to practical steps to prevent contamination, Michael shares actionable insights to help laboratories achieve accuracy, consistency, and confidence in their workflows.
During an LC-MS/MS experiment, traditional fragmentation techniques like collision-induced dissociation (CID) have long been the gold standard. Electron-activated dissociation (EAD) is emerging as a transformative tool that enhances structural elucidation, particularly for complex or labile metabolites.
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