Separating cyclosporin isomers using differential mobility separation (DMS) by Pierre Negri | Aug 7, 2021 | Data acquisition, Differential Mobility Separation (DMS), SCIEX information, TechnologyCyclosporin A (CsA) has been a very successful immunosuppressant for preventing organ transplant rejection. It is a large cyclic peptide (11 residues, ~1200 Da) and there are numerous modifications of this drug, some of which are isomers. The challenge of analyzing...
Using Bio Tool Kit for interpreting peptide MS/MS data obtained using electron activated dissociation (EAD) by Christie Hunter | Aug 7, 2021 | Bio Tool Kit, Biopharma, Data processing, Data Visualization, Life Science Research, Proteomics, QTOF, SCIEX information, Technology, ZenoTOF 7600 systemPeptide MS/MS spectra obtained using either collision induced dissociation (CID) or electron activated dissociation (EAD) can be analyzed using the Peptide Fragments pane in Bio Tool Kit in SCIEX OS Software. First, open the EAD MS/MS spectrum that you want to...
Peptide fragmentation using electron activated dissociation (EAD) by Christie Hunter | Aug 7, 2021 | Bio Tool Kit, Biopharma, Data acquisition, Life Science Research, Proteomics, SCIEX information, Technology, ZenoTOF 7600 systemMost workflows in protein quantification and characterization by mass spectrometry have used collision-induced dissociation (CID). In the CID process, a peptide is selected in the first quadrupole of the instrument (Q1), then accelerated through a collision cell where...
Automatic calibration in minutes for microflow LC with the OptiFlow source on ZenoTOF 7600 system by Alexandra Antonoplis | Aug 7, 2021 | Bioanalysis/PK, Biopharma, Data acquisition, Ion sources, Life Science Research, Metabolomics, Pharma, Proteomics, SCIEX information, Technology, ZenoTOF 7600 systemThe OptiFlow ion source for the SCIEX ZenoTOF 7600 system is equipped with an ESI calibration probe for automatic calibration during microflow experiments (diagram below). The ESI probe is installed orthogonal to the microflow probe in the OptiFlow source and...
What is the Zeno threshold and what does it do? by Robert Di Lorenzo | Aug 7, 2021 | Bioanalysis/PK, Data acquisition, Lipidomics, Metabolomics, Multi-Omics, Proteomics, SCIEX information, SCIEX OS software, Technology, Toxicology, ZenoTOF 7600 systemWith the introduction of the Zeno trap on the ZenoTOF 7600 system, sensitivity improvements on the order of 4 to 25x can be seen for MS/MS analysis, because of the optimization of duty cycle.1 When concentrations are low and peak intensities are limited, this bump in...