See How Easy It Can Be to Get Expert Results for Biologics Characterization

Feb 7, 2017 | Biopharma, Blogs | 0 comments

Learning a new mass spec system can be a daunting task. Aside from the opportunity costs of training new users, you might face the hassle of downtime, and the wait to get expert help when needed. The X500B QTOF system puts a new spin on biologics characterization workflows because it is so easy to learn and operate that you can be up and running much faster than you expect. Powerful new software tools dramatically streamline method development and data processing, to enable everyone in your lab to get expert results. It’s fast because it’s easy, even for new users.

Intact Mass Analysis: Rapid Answers with Confidence
New BioPharmaView™ Software 2.0 combines perfectly with the X500B QTOF system, to put powerful data processing tools for visualization and comparisons at your fingertips. You only need to provide protein sequence and potential modification information, since BioPharmaView Software automates intact protein deconvolution in seconds. In BioPharmaView software you’re able to:

  • Reduce variability and improve comparability
  • Rapidly interpret your data with powerful graphical post-translational modification analysis
  • Display your raw data alongside deconvoluted output for more confident answers

Discover how automated, and lightning fast drug-antibody ratio (DAR) calculations in BioPharmaView Software allow for easy sample comparison, so you can spend less time on manual calculations and more time on visualizing drug load. Add to that easy one-click batch processing and automated ratio calculations for post-translational modification analysis. You’ll see how straightforward it is to rapidly identify and visualize differences between samples to make direct comparisons in either the raw MS spectra or deconvoluted protein results.

Peptide Mapping: Unbiased Comprehensive Analysis
SCIEX OS operating software built into the X500B QTOF system makes comprehensive peptide mapping easier with new workflows: simplified acquisition and streamlined peptide mapping analysis ensure everyone can get great results with just a few clicks.

The X500B system supports traditional Information or Data-Dependent Acquisition (IDA or DDA) methods, as well as proprietary SWATH® Information Independent Acquisition (IDA) for comprehensive identification of even low abundance peptides and post-translational modifications. SWATH Acquisition employs an unbiased acquisition strategy, where every precursor is targeted for MS/MS analysis, so you don’t need to worry about missing important attributes.

Using the X500B system and BioPharmaView Software, you’ll be able to analyze peptide mapping data in just four steps using, so you can make better decisions faster about your biologics products:

  1. Define your biotherapeutic sequences and disulfide bonds
  2. Add custom modifications for samples like ADCs
  3. Save your assay information for batch processing
  4. Generate comprehensive reports in multiple output formats

Fill out the form in the top right of this blog to download the Intact Mass Analysis and Peptide Mapping Solution Guides. These guides show how improved data acquisition and analysis workflows with SCIEX OS and BioPharmaView Software for the X500B QTOF System can transform your biotherapeutic characterization.


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