GEN-MKT-18-7897-A
Jan 2, 2019 | Biopharma, Blogs | 0 comments
As interest in N-glycan analysis grows within the biopharma industry, innovation continues allowing analyses to be done in less time with fewer and less tedious, sample preparation steps. One of these recent innovations was the release of the SCIEX Fast Glycan Technology which offers a simplified 60-minute sample prep, 5-minute separations, and automatic glycan identification. But what happens when you have an unknown peak in your sample, or if you need to confirm the structural identity of a critical glycan species? Well, developments in N-glycan analysis has continued with new methods for N-Glycan sequencing which provide complete structural elucidation in as little as 1 hour.
Having Problems With Glycan Sequencing?Previously, carbohydrate sequencing was a tedious and time-consuming manual process. It required either consecutive addition of sequencing exoglycosidase enzymes to a single vial, which could take up to a week, or arrays of enzymes in multiple vials and parallel processing of samples which could still take a full day.
Combatting Glycan Sequencing Challenges: To break through the problems associated with older sequencing methods, two different approaches have been developed that significantly decrease manual steps and reduces the analysis time from 1–5 days to 1–2 hours. These are both semi- and fully-automated methods which can be used on your existing PA 800 Plus Pharmaceutical Analysis System together with the award-winning Fast Glycan Technology to give you the reliable data you need faster.
Differences Between Semi- and Fully- Automated Methods: The semi-automated, array-based sequencing requires only 1 hour (including reaction times, digestions, and the CE-LIF separations). However, manual pre-mixing of the enzyme mixtures is required, as well as a higher concentration of your IgG1 sample. The fully automated workflow does not require any pre-analysis of reaction arrays, but due to its serial nature, the total sequencing time was just over 2 hours. The flexibility to choose between these methods means that you can optimize your analysis for speed or ease of use depending on your needs.
If you’re interested in learning more about the new automated N-glycan sequencing methods, fill out the form on the right to watch the webinar, Sequence Your Biopharma N-Glycans in as Little as 1 Hour.
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The Echo® MS+ system is a novel platform for Acoustic Ejection Mass Spectrometry (AEMS) and combines the speed of acoustic sampling with the selectivity of mass spectrometry. This platform has been designed for high throughput analysis of small and large molecules. The technology combines Acoustic Droplet Ejection (ADE), an Open Port Interface (OPI) and could be coupled with the SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system.
The Echo® MS+ system comprises of an open-port interface (OPI) and acoustic droplet ejection (ADE) module which could be coupled with a mass spectrometer. The mass spectrometer could either be a SCIEX Triple Quad 6500+ system or the ZenoTOF 7600 system. This non-liquid chromatography based; high-throughput screening platform enables rapid analysis of compounds at speeds of up to 1 sample/second.
The ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
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