In this set of CloudTalks, you will gain an understanding about all the tools available in the OneOmics suite. Watch the first video see a high level review of the OneOmics suite. Watch the second video to get a short tour of the platform to gain an...
This excel sheet allows you to quickly estimate the MRM concurrency and approximate dwell time for your Scheduled MRM Algorithm acquisition method. Paste your MRM method with retention times into the input tab then calculate the Excel workbook. Plots will update to...
Finding interesting and significant changes across a biological dataset can be challenging, so having good algorithms that can mine large datasets for differences, and find the statistically significant changes is really important. Within the MarkerView App we have...
General points: Use of laboratory deionized (DI) water is specified at points in the procedure. However, if there are any concerns regarding quality, please utilize a reputable supplier of pure deionized water (>18.2M ohm). 5-10 liters will typically be required...
Assessing the linear response of ITC (dynamic ion transmission control) is an easy way to determine whether you should clean your QJet ion guide and IQ0 lens. A clean rod set and lens should give a linear response when ramping the ITC parameter and the ratio of 132...
Collision energy spread (CES) generates a collision energy (CE) ramp around a collision energy center point, with the goal of providing a more, rich MS/MS spectrum of your analytes. The CE center point is either set in your acquisition method or computed on the fly...
The key performance attributes for qualitative analysis are speed, mass accuracy, and resolution in both MS and MS/MS modes. For quantitative analysis, inter-scan dynamic range is important in order to maintain accuracy and linear response over a wide range of sample...
Dynamic Ion Transmission Control (ITC) is the feature on all the QTOF systems from SCIEX where the MS ion current through the instrument is modulated scan by scan to ensure the detector saturation point is not reached. The modulation is done both at the level of total...
The MRM3 workflow and the MS3 scan are functionally the same QTRAP system scan, but used with different goals in mind. The main difference is how these scans are used in the whole MS workflow. With MS3 scans, you can use these in a data dependent mode for discovery...
Using the dual gradient system, the nanoLC 425 system can be configured such that the trap can be washed to waste rather than through the column and into the mass spectrometer. This workflow can be performed with either nanoflow or microflow chromatography. Figure 1....
Peptide MS/MS spectra obtained using either collision induced dissociation (CID) or electron activated dissociation (EAD) can be analyzed using the Peptide Fragments pane in Bio Tool Kit in SCIEX OS Software. First, open the EAD MS/MS spectrum that you want to...
Most workflows in protein quantification and characterization by mass spectrometry have used collision-induced dissociation (CID). In the CID process, a peptide is selected in the first quadrupole of the instrument (Q1), then accelerated through a collision cell where...