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Author Highlights

Christie Hunter

Christie Hunter

Christie Hunter is the Director of Applications at SCIEX. Christie has worked at SCIEX for 20 years, pioneering many workflows in quantitative proteomics. Christie was an early user of SWATH acquisition and played a big role in evolving the workflows and driving adoption of this new data independent approach with many proteomic researchers. Christie and her team are focused on developing and testing innovative MS workflows to analyze biomolecules, and work collaboratively with the instrument, chemistry and software research groups.
What is SWATH acquisition and what are the critical acquisition attributes?

What is SWATH acquisition and what are the critical acquisition attributes?

In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition,...

Files for running the SWATH acquisition performance kit – ion library, performance trackers

Files for running the SWATH acquisition performance kit – ion library, performance trackers

The FASTA file required for use with the SWATH acquisition performance kit installs with ProteinPilot software or is available within OneOmics suite. Download this SWATH acquisition ion library for use with the SWATH acquisition performance kit Human2D_SOP_PepCalMix_...

Adapting a SCIEX high flow source for microflow LC

Troubleshooting low flow LC – Loss of early eluting peptides during trap-elute workflow

Symptom: Early eluting peptides are missing (based on monitoring PepCalMix LC-MS test or observing a change in the shape of the proteome TIC). Test: Use PepCalMix LC-MS test to monitor binding of early eluting peptides. There are a number of very hydrophilic peptides...

CloudTalk – Analytics for proteomics

CloudTalk – MarkerView app for proteomics and metabolomics

The MarkerView app in OneOmics suite allows you to use various multivariate analysis techniques to explore similarities and differences within an experiment or between experiments. Both supervised and unsupervised methods can be used. This CloudTalk video (11:42mins)...

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