calibration using sum of peak areas vs concentration by Andrew Charlton | Sep 12, 2023 | Data processing, QTRAP / Triple Quad, Questions, SCIEX OS softwareHi,
Reporting only unknown samples containing a specific sample ID prefix (in this case Sample ID prefix = PHT) by Steven McGuire | Jul 24, 2023 | Data processing, QTRAP / Triple Quad, Questions, SCIEX OS softwareI am using Multiquant software and I am trying to create a report to pull data on specific sample from a large run.
ProteinPilot phosphopeptide library and DIA-NN by Roz Jenkins | May 25, 2022 | Data processing, Life Science Research, ProteinPilot, Proteomics, QTOF, QuestionsI have prepared a spectral library for a phosphopeptide enriched sample and I am generating my SWATH samples from similarly enriched samples. The problem is that when I use DIA-NN for the retention time alignment and quant, it doesn’t recognise the terminology of the spectral library annotated by ProteinPilot. DIA-NN recognises Unimod:21 for phosphorylation, but PP uses phospho(Tyr) etc. Other than changing the data dictionary to get around the mismatch, anyone have any suggestions for how I might resolve this? Thank you, Roz
Current proteomics software compatibility for ZenoTOF 7600 system by Christie Hunter | May 20, 2022 | Data processing, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemBelow is a summary of various other software packages that are useful for processing proteomics data from the ZenoTOF 7600 system. Note this list is not comprehensive and only covers the tools we have lightly tested to date. Acquisition Type Software Files needed...
Sequential processing of multiple data-files in ProteinPilot by Adrian Brown | May 12, 2022 | Data processing, ProteinPilot, Proteomics, QTOF, QuestionsI would like to use ProteinPilot 5.0.2 to process data-sets containing 16 wiff files acquired from fractionated peptides on a 6600 TripleTOF. A Precision T7910 workstation struggles to process four files in parallel and I would like to be able to queue sequential processing of individual files overnight. I currently use the ‘LC ‘ tab to load and process individual data-files but this leads to parallel processing. Is it possible to generate 16 .group files sequentially?
Creating a library from a FASTA file for library-free data analysis by Christie Hunter | Apr 29, 2022 | Data processing, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemWhile generating proteomic spectral libraries today is relatively easy, sometimes a library is needed and there is not time or need to generate a deep library using the traditional data-dependent acquisition approach. Here, a strategy will be outlined to generate a...
Processing ZenoTOF 7600 system data with DIA-NN software by Christie Hunter | Apr 29, 2022 | Data processing, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemDIA-NN software is a powerful software tool for processing data independent acquisition (DIA) proteomics datasets. It uses neural networks and other algorithms to identify and quantify peptides and proteins from DIA data and is specifically optimized for fast...
Excel macro for calculating averages or percent CVs from replicates in a 384 -well plate by Mackenzie Pearson | Feb 24, 2022 | Bioanalysis/PK, Data processing, Data Visualization, Echo MS, Pharma, SCIEX information, SCIEX OS softwareThis excel workbook is designed to be used with data copied from a SCIEX OS software Analytics module results file
Uploading data for use in OneOmics suite by Alexandra Antonoplis | Dec 14, 2021 | Data processing, Life Science Research, Metabolomics, Multi-Omics, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThere are two options for data storage when working with the OneOmics suite. You can store your data either within the SCIEX Cloud platform in the Data Store, or you can store data in BaseSpace (Illumina) and link your BaseSpace account with the SCIEX Cloud platform....
sMRM Pro Builder template tutorial by Mackenzie Pearson | Nov 15, 2021 | Data processing, Data Visualization, How-to videos, Life Science Research, Lipidomics, Metabolomics, Optimizing Performance, QTRAP / Triple Quad, ResultsThe sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.