GEN-MKT-18-7897-A
Dec 16, 2016 | Blogs, Food / Beverage | 0 comments
There is nothing like the flavor of fruit juice whether freshly squeezed or made from concentrate to clench your thirst, except when it’s not 100 percent juice after all. As the following tech note, “Authenticity Assessment of Fruit Juices using LC-MS/MS and Metabolomic Data Processing,” (pgs.135-141) points out, such substances have become a likely target for fraud and adultery practice even though certain legislation measures dictate authenticity parameters.
Why should we care? Consumers love to buy fruit juice. In a report by New Scientist, Britain sells more than 800 million pounds per year, while the U.S. holds it stakes at $12 billion or 7.5 billion pounds. Important numbers since 16 out of 21 brands of orange juice sold in Britain contained false ingredients such as beet sugar.Download The Food Compendium >
The problem with existing methods, however, is they are limited to targeted approaches useful when testing one or a handful of adulterations and researchers need to cast a wider net to keep up with the numerous compounds being added to the products. Using LC-MS/MS techniques with the QTRAP® and TripleTOF® systems researchers were able to capture non-targeted results for proof of authenticity. Included in the application note are details right down to where the juices were purchased to sample prep and results.
Why download the compendium? Gain access to almost 200 pages and 18 application methods that respond to real world problems like the ones discussed here. For example, how will you bring these solutions into your lab and improve upon current testing methods? Branch out with new and improved methods you can only find here. It’s just one way you can help prevent the food fraudulence.
Finding the right information shouldn’t slow you down. Whether you’re troubleshooting your mass spec, learning something new, or optimizing performance, access to the right resources at the right moment makes all the difference.
As an analytical strategy, middle-down mass spectrometry (MS) workflows characterize biotherapeutic proteins by analyzing large, digested protein fragments or defined subunits, rather than fully intact proteins (top-down) or digested peptides (bottom-up). A middle-down strategy combines the strengths of top-down and bottom-up approaches by delivering high sequence coverage and structural specificity while maintaining relatively simple sample preparation. In practice, middle-down analysis enables accurate mass measurement, rapid sequence confirmation, and localization of key post-translational modifications (PTMs) on protein subunits that are directly relevant to product quality.
In biopharmaceutical development, sequence variants (SV) are considered an inherent risk of producing complex proteins in living systems. Sequence variants are unintended changes to the amino acid sequence of a biotherapeutic and can be caused by errors in transcription or translation in the host cell, or cell culture and process conditions. Detailed analysis of SVs is important in process and product development to ensure the drug’s safety and efficacy. Even low‑level sequence variants can have significant implications for product quality, safety, and efficacy, making their accurate detection and characterization a critical requirement across development, process optimization, and regulatory submission.
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