Data acquisition on the Echo® MS system: post-acquisition file splitting by Anuja Bhalkikar | Sep 14, 2023 | Data acquisition, Echo MS, SCIEX information, TechnologyThe Echo® MS system is an ultra-high-throughput system that analyzes samples at a speed of up to 1 sample per second. To achieve fast acquisition speeds, MS data is collected for an entire batch—or the entire well plate—into a single experiment in a file....
Please provide at least 3 decimal places for expected retention time for scheduled MRM at EchoMS by Riyo Imamura | Jun 22, 2023 | Data acquisition, Echo MS, Questions, SCIEX OS software, TechnologyExpected retention time for scheduled MRM at EchoMS must have at least 3 decimal places, because the minute display with two decimal places is insufficient to show the difference in seconds. Thank you.
General question on SCIEX 7500 by Dhayaalini Nadarajan | May 1, 2023 | Data acquisition, Ion sources, Questions, SCIEX OS software, TechnologyGood morning.
Optimized rolling collision energy curves for IDA and SWATH DIA for peptides by Christie Hunter | Apr 18, 2023 | Data acquisition, Life Science Research, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemDuring data dependent acquisition (DDA or IDA) or SWATH acquisition, the collision energy can be automatically adjusted according to the mass/charge and charge of the peptide. This dependency has been well characterized on our QTOF systems. By selecting rolling...
Easy switching of sources and LC flow regimes on the ZenoTOF 7600 system by Christie Hunter | May 9, 2022 | Data acquisition, Ion sources, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemThis series of videos outlines how a user can easily switch sources and operate the system in different LC flow regimes.
Setting up custom on-column nanoflow calibration for the ZenoTOF 7600 system using PepCalMix by Alexandra Antonoplis | Apr 29, 2022 | Data acquisition, Life Science Research, Proteomics, SCIEX information, SCIEX OS software, ZenoTOF 7600 systemFor nanoflow workflows using the ZenoTOF 7600 system and the standard nanoflow interface, on-column calibration is required and can be incorporated automatically into the SCIEX OS software acquisition batch. Here, step-by-step instructions are provided to set up a...
Using Scheduled Ionization to reduce system ion load for proteomics data acquisition by Alexandra Antonoplis | Apr 29, 2022 | Data acquisition, Ion sources, Life Science Research, Proteomics, QTOF, QTRAP / Triple Quad, SCIEX information, SCIEX OS software, ZenoTOF 7600 systemWhen analyzing highly complex samples from biological matrices, there can be significant amounts of material that elute in the wash cycle of the LC run, depending on the up-front sample preparation used. The Scheduled Ionization mode, available in both SCIEX OS...
Excel macro for plate building for transformation of 96-well to 384-well plates with generation of batch lists for SCIEX OS software by Mackenzie Pearson | Feb 24, 2022 | Bioanalysis/PK, Data acquisition, Echo MS, Pharma, Research (RUO), SCIEX informationThis macro-enabled workbook is designed to help with creating a formatted analysis list for the Echo® MS system, using 96-well plate maps or lists
Standard mixture for bare fused silica capillary. by Jose Rodriguez-Martinez | Feb 15, 2022 | Biopharma, CE, Data acquisition, Optimizing Performance, QuestionsHi, is there a recommended standard mixture to evaluate the performance of CZE runs in a bare fused capillary with UV/Vis detector? Thanks.
How to optimize sample plating to run Echo MS system in ‘Fast acquisition mode’ by Mackenzie Pearson | Jan 31, 2022 | Data acquisition, Echo MS, Optimizing Performance, Pharma, SCIEX information, TechnologyThe Echo MS system can acquire data extremely rapidly, at 1 second per sample. To achieve this speed, it should be ensured that there is adequate time between ejections for the analyte signal to return to baseline between ejections. Plating your samples such that you...