In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition,...
The FASTA file required for use with the SWATH acquisition performance kit installs with ProteinPilot software or is available within OneOmics suite. Download this SWATH acquisition ion library for use with the SWATH acquisition performance kit Human2D_SOP_PepCalMix_...
To set up a SCIEX high flow source for microflow LC (Turbo V ion source, DuoSpray source or IonDrive Turbo V ion source), first you must replace the wider bore electrodes with more narrow bore hybrid electrodes. Note with the OptiFlow Turbo V ion sources, there are...
Symptom: LC-MS peptide peak are is smaller than expected in PepCalMix Test Test: Use PepCalMix Infusion tests to determine if it is a problem with LC or MS Solution: First perform the SCIEX recommended performance tests using infusion on your MS system. If these...
Symptom: The observed retention times of the peptides eluting are compressed, are not as spread out as expected, suggesting the elution gradient is compressed Test: Use PepCalMix LC-MS test to check the peptide elution time and spread Solution: Make sure you are...
Symptom: Early eluting peptides are missing (based on monitoring PepCalMix LC-MS test or observing a change in the shape of the proteome TIC). Test: Use PepCalMix LC-MS test to monitor binding of early eluting peptides. There are a number of very hydrophilic peptides...
This CloudTalk video (6:34min) describes the use of the Analytics app in OneOmics suite for evaluating the quality of your SWATH acquisition data. RUO-MKT-18-6582-A
This CloudTalk (6:57min) covers the basics of the Review app in the workspaces, for reviewing your multi-omics data in OneOmics suite. RUO-MKT-18-7017-A
The MarkerView app in OneOmics suite allows you to use various multivariate analysis techniques to explore similarities and differences within an experiment or between experiments. Both supervised and unsupervised methods can be used. This CloudTalk video (11:42mins)...
This video (11:37min) describes the use of the Browser app in OneOmics suite for visualizing the protein expression data from your SWATH acquisition data. RUO-MKT-18-6583-A
This CloudTalk describes the use of the Assembler app in the OneOmics suite for normalization and computing fold change from SWATH acquisition data. Learn the specifics about these algorithms in this short video. To visualize your SWATH DIA results, please watch these...
This CloudTalk describes the use of the Extractor app in the OneOmics suite for the processing the SWATH acquisition data. RUO-MKT-18-6593-B