Useful FAQ document to enable researchers to focus on their scientific discoveries and insights rather than the complexities of data management.
Lipidomics
Collaborate with your peers. Pose and answer questions or review responses about the use of mass spectrometry and the analysis of lipids here. Discover how to get started on lipid sample preparation techniques and results.
Mass spec data across the cultivated meat value chain
We recently had the opportunity to chat with Alex Ward, PhD, Principal Consultant, Arta Bioanalytics to discover more about his work in developing transcriptomics and metabolomics data interpretation for the cultivated meat sector. As a specialist in multi-omics approaches, Alex is driving R&D, production and regulatory processes for the industry. Below are Alex’s responses to a few questions we asked, sharing his knowledge with the SCIEX community to drive the future of cultivated meat.
New features in OneOmics suite
I just wanted to thank the readers here, both the OneOmics suite users who’ve shared their time and watched OneOmics grow, and for all the talented developers and scientists who’ve made OneOmics suite what it is today.
Get started with these easy-to-implement methods for global lipid profiling
Lipidomics Quantification of lipids in complex biological samples is challenging and often requires a detailed knowledge of lipids and lipid analysis methods. The inherent complexity of the lipidome means that identifying and quantifying different lipid classes and...
sMRM Pro Builder template tutorial
The sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.
High complexity of the lipidome
The complexity of the lipidome is diverse in the structure and there are many combinatorial isoforms that are available within nature. Currently, many different techniques are required to fully characterize a lipid molecule. What if you could do it in a single...
What is the Zeno threshold and what does it do?
With the introduction of the Zeno trap on the ZenoTOF 7600 system, sensitivity improvements on the order of 4 to 25x can be seen for MS/MS analysis, because of the optimization of duty cycle.1 When concentrations are low and peak intensities are limited, this bump in...
Tips and tricks: setting up LC-MS/MS methods for lipid mediator profiling
Starting up a workflow for lipid mediator analysis can be challenging. Hundreds of distinct fatty acid-derived molecules can be produced biologically (i.e., enzymatically) and/or via non-enzymatic routes. Additionally, various biosynthetic pathways yield both...
Separation anxiety: Choosing the best approach to quantify the lipidome
Front-End Solutions for Lipid AnalysisWhich method should you choose? Lipids are a diverse group of compounds that serve many purposes. They are the basis for key biological entities including cell membranes, hormones, and lipoproteins and are involved in a variety...
The Latest and Greatest for Improving Lipidomic Analysis by Mass Spectrometry
Differential Mobility Separation (DMS) resolves multiple lipid classes within complex lipid matrices prior to MS analysis to enable more confident identification of lipid species and more accurate quantitation by MS/MS. Lipidomics research has progressed rapidly in...