This excel workbook is designed to be used with data copied from a SCIEX OS software Analytics module results file If there are multiple results for a sample, the data should be sorted so that well replicates are in sequence with each other. If they are out of order,...
Author Highlights
Mackenzie Pearson
Excel macro for plate building for transformation of 96-well to 384-well plates with generation of batch lists for SCIEX OS software
This macro-enabled workbook is designed to help with creating a formatted analysis list for the Echo® MS system, using 96-well plate maps or lists How to use: For 96-well plate sample lists using the numbered format location (1 to 96, instead of A1 to H12) Copy sample...
How to optimize sample plating to run Echo MS system in ‘Fast acquisition mode’
The Echo MS system can acquire data extremely rapidly, at 1 second per sample. To achieve this speed, it should be ensured that there is adequate time between ejections for the analyte signal to return to baseline between ejections. Plating your samples such that you...
Selecting the mobile phase for your assay and optimizing flow rates
To obtain the best, most reproducible results with Echo MS system, it is important both to select the best solvent for your analyte and matrix and to ensure the flow rate is optimized for your solvent. Considerations for solvent selection: The Echo MS system is...
sMRM Pro Builder template tutorial
The sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to...
High level method optimization considerations for Echo MS system
While an in-depth discussion of method development and optimization for the Echo® MS system is beyond the scope of a community post, here are some points to consider as part of the process: The maximum recommended ion spray voltage for prolonged electrode life is 5000...
What to do if you see peak shape deterioration or jagged peaks in your Echo MS system data?
Once a method has been developed, and good, consistent peak shapes have been obtained for an assay, the Echo® MS system is generally quite consistent. If peak quality deteriorates, consider the following points: The viscosity of the sample can affect the peak shape...
Tips to maximize electrode lifetime for Echo MS system
While it’s easy to think of the Echo® MS system as an ultrafast LC system in front of the SCIEX Triple Quad 6500+ mass spectrometer, the system operates on fundamentally different principles. For this reason, it requires different routine maintenance to keep it...
High complexity of the lipidome
The complexity of the lipidome is diverse in the structure and there are many combinatorial isoforms that are available within nature. Currently, many different techniques are required to fully characterize a lipid molecule. What if you could do it in a single...
Tips and tricks: setting up LC-MS/MS methods for lipid mediator profiling
Starting up a workflow for lipid mediator analysis can be challenging. Hundreds of distinct fatty acid-derived molecules can be produced biologically (i.e., enzymatically) and/or via non-enzymatic routes. Additionally, various biosynthetic pathways yield both...
Separation anxiety: Choosing the best approach to quantify the lipidome
Front-End Solutions for Lipid AnalysisWhich method should you choose? Lipids are a diverse group of compounds that serve many purposes. They are the basis for key biological entities including cell membranes, hormones, and lipoproteins and are involved in a variety...
The Latest and Greatest for Improving Lipidomic Analysis by Mass Spectrometry
Differential Mobility Separation (DMS) resolves multiple lipid classes within complex lipid matrices prior to MS analysis to enable more confident identification of lipid species and more accurate quantitation by MS/MS. Lipidomics research has progressed rapidly in...