Computing protein confidence with improved accuracy by reassessing peptide confidence during protein grouping by Christie Hunter | 0 CommentsProteinPilot software 4.0 and higher releases include a new method for calculating protein confidences that improves reliability at the end of protein lists. Figure 1 shows a simulated example that demonstrates how more accurate protein confidence is computed. Figure...
What’s in your citrus oil? by Craig Butt | 0 CommentsCraig Butt explains a non-targeted omics approach to characterizing and profiling compounds in citrus oil Read time: 4 minutes There is increasing interest among consumers in the benefits of natural products containing citrus beyond the traditionally known benefits of...
Breaking down the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready by Jianru Stahl-Zeng | 0 CommentsSensitivity and robustness carry different meanings in the world of mass spectrometry. Generally, sensitivity refers to an instrument’s ability to achieve lower limits of detection (LOD). Robustness, on the other hand, refers to an instrument’s ability to consistently...
The honey sting by Katherine Hyland | 0 CommentsAs a consumer it’s hard for me not to feel inundated with claims that our food is “all-natural” or “chemical-free” or that we should buy certain “superfoods” for their health benefits. We read labels and trust that the product we are buying is what we are truly...
Your success and voice go a long way! by Philip Taylor | Biopharma, Blogs, Clinical, Environmental / Industrial, Food / Beverage, Forensic, Life Science Research, Pharma, TechnologyAt the heart of everything we do is ensuring that your workflows and team are empowered to achieve optimal results with your SCIEX instruments, software, consumables, and services. Every interaction with SCIEX is designed to support your success through the dedication...
An overview: LC-MS analysis of targeted protein degraders and their metabolites by Kirsten Craven | Blogs, Pharma, ZenoTOF 7600 systemTargeted protein degraders (TPD) are a relatively new therapeutic modality that opens the potential to target disease-causing proteins. These disease-causing proteins have been highly challenging for traditional small-molecule therapeutics to treat, making TPDs an exciting new therapeutic modality.
Optimized rolling collision energy curves for IDA and SWATH DIA for peptides by Christie Hunter | Data acquisition, Life Science Research, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemDuring data dependent acquisition (DDA or IDA) or SWATH acquisition, the collision energy can be automatically adjusted according to the mass/charge and charge of the peptide. This dependency has been well characterized on our QTOF systems. By selecting rolling...
Current proteomics software compatibility for ZenoTOF 7600 system by Christie Hunter | Data processing, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemBelow is a summary of various other software packages that are useful for processing proteomics data from the ZenoTOF 7600 system. Note this list is not comprehensive and only covers the tools we have lightly tested to date. Acquisition Type Software Files needed...
Using Scheduled Ionization to reduce system ion load for proteomics data acquisition by Alexandra Antonoplis | Data acquisition, Ion sources, Life Science Research, Proteomics, QTOF, QTRAP / Triple Quad, SCIEX information, SCIEX OS software, ZenoTOF 7600 systemWhen analyzing highly complex samples from biological matrices, there can be significant amounts of material that elute in the wash cycle of the LC run, depending on the up-front sample preparation used. The Scheduled Ionization mode, available in both SCIEX OS...
Excel macro for plate building for transformation of 96-well to 384-well plates with generation of batch lists for SCIEX OS software by Mackenzie Pearson | Bioanalysis/PK, Data acquisition, Echo MS, Pharma, Research (RUO), SCIEX informationThis macro-enabled workbook is designed to help with creating a formatted analysis list for the Echo® MS system, using 96-well plate maps or lists
Rescheduling a Schedule I substance, and the Delta-8 controversy by Diana Tran | Blogs, Food / Beverage, Food and BeverageDid you know that in the US, drugs and other chemicals are classified into 5 distinct categories depending on the drug’s acceptable medical use and its potential for abuse or dependency? Drugs federally classified as Schedule I substances by the US Drug Enforcement Administration (DEA) are considered to have the highest potential for abuse and for creating severe psychological and/or physical dependence. In addition to heroin, LSD and MDMA (ecstasy), cannabis is classified as a Schedule I substance in the Controlled Substance Act of 1970, which means it has no approved medical usage.
Uploading data for use in OneOmics suite by Alexandra Antonoplis | Data processing, Life Science Research, Metabolomics, Multi-Omics, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThere are two options for data storage when working with the OneOmics suite. You can store your data either within the SCIEX Cloud platform in the Data Store, or you can store data in BaseSpace (Illumina) and link your BaseSpace account with the SCIEX Cloud platform....
sMRM Pro Builder template tutorial by Mackenzie Pearson | Data processing, Data Visualization, How-to videos, Life Science Research, Lipidomics, Metabolomics, Optimizing Performance, QTRAP / Triple Quad, ResultsThe sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.
MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system by Christie Hunter | Bioanalysis/PK, Data processing, Methods / workflows, Pharma, QTRAP / Triple Quad, SCIEX information, SCIEX OS softwareGeneral recommendations when beginning method development Objective: The purpose of this document is to provide a quick reference for transferring MRM-based quantification methods from a SCIEX Triple Quad or QTRAP 6500+ system to a SCIEX 7500 system. While the best...