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What is the Zeno threshold and what does it do?

With the introduction of the Zeno trap on the ZenoTOF 7600 system, sensitivity improvements on the order of 4 to 25x can be seen for MS/MS analysis, because of the optimization of duty cycle.1 When concentrations are low and peak intensities are limited, this bump in...

What is SWATH acquisition and what are the critical acquisition attributes?

What is SWATH acquisition and what are the critical acquisition attributes?

In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition,...

What’s in your citrus oil?

What’s in your citrus oil?

Craig Butt explains a non-targeted omics approach to characterizing and profiling compounds in citrus oil Read time: 4 minutes There is increasing interest among consumers in the benefits of natural products containing citrus beyond the traditionally known benefits of...

A rising star in food allergen research: proteomics of shellfish allergen

A rising star in food allergen research: proteomics of shellfish allergen

It’s important to know what you’re eating, especially if you suffer from a food allergy.

About 220 million people worldwide live with a food allergy.1 These numbers, along with the complexity and severity of conditions, continue to rise. In America, there are about 32 million food allergy sufferers—5.6 million of those are children under the age of 18.2.2 That’s 1 out of every 13 children, or about 2 in every classroom. From a financial perspective, the cost of food allergy childcare for US families is up to $25 billion

Integration of Electrophoresis into a Single, “Plug-and-Spray” Device Offers a New Approach for Metabolomics Applications

Integration of Electrophoresis into a Single, “Plug-and-Spray” Device Offers a New Approach for Metabolomics Applications

Teasing apart the metabolome: CESI-MS separation of small, anionic compounds
Metabolomics, an emerging field focused on the chemical processes central to cellular metabolism gives scientists a snapshot of the cellular metabolic state, or metabolome at a given time. Highly complex, the metabolome contains a large and diverse group of small molecules and requires a systematic understanding of chemical end-products of cellular processes.  Used in conjunction with genomics and proteomics information and researchers can gain a deeper understanding of an organism’s physiology. As a result, insights on a disease’s progress or an organism’s health status become clearer. However, there is a caveat. Teasing apart metabolome components and identifying each molecule is a significant analytical challenge that requires specialized applications and approaches able to separate very small, chemically similar analytes from each other.

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