Pathway to Success: Young Metabolomics Researchers Named in The Analytical Scientist’s Power List by SCIEX Community | 0 Comments The direct correlation of the metabolome to the phenotype means metabolomics is one of the most sought-after approaches for the study of disease and wellness, yet the separation, detection, quantification, and unambiguous identification of a chemically diverse...
3 Dicamba Analysis Questions You Need to Ask Yourself Today by SCIEX Community | 2 CommentsWe blinked and the last two years went in a flash. It seems like just yesterday, the Dicamba herbicide controversy hit the headlines in 2016 and the EPA set the regulations to expire in two years. Fast forward to today. Dicamba is an acid herbicide used to kill...
Don’t Be a Food Scandal Scapegoat. Fight Back with LC-MS/MS for Your Meat Authenticity Testing by SCIEX Community | 0 CommentsThe infamous horsemeat scandal of 2013 shined a spotlight on the questionable integrity of our meat supply. What followed was a lengthy investigation as the extent of the scandal unraveled before our eyes, reaching far beyond the European borders...
It’s Time to Turn Things Around―Faster and Accurate Mass Analysis of Organic Explosives by SCIEX Community | 0 CommentsSadly, the world is a volatile place. The threat of organic explosive use by terrorists and criminals is very much with us, posing a significant danger to people worldwide. The rising concern for public safety is a top priority for national security organizations...
Pharma perspectives: The influence of LC-MS innovation on drug development outsourcing by Kean Woodmansey | Blogs, Pharma, ZenoTOF 7600 systemIt is no secret that (bio)pharmaceutical research and development is complex, both scientific and regulatory processes. Working for a contract research organization and more recently for SCIEX has provided an interesting perspective on trends the market experiences that affect many of us.
Nitrosamines: Where are we now? by SCIEX Community | Blogs, Pharma, QTRAP / Triple QuadNitrosamines are a large group of N-nitroso compounds that share a common functional N-N=O group. They are produced by a chemical reaction between a nitrosating agent and a secondary or tertiary amine. Back in 2018, nitrosamines suddenly found themselves in the spotlight when they were unexpectedly detected in medications for high blood pressure. Since then, they have been found in several other prescription medications, including those for heartburn, acid reflux and diabetes, resulting in manufacturers recalling some common medications.
Easy switching of sources and LC flow regimes on the ZenoTOF 7600 system by Christie Hunter | Data acquisition, Ion sources, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemThis series of videos outlines how a user can easily switch sources and operate the system in different LC flow regimes.
Thailand cannabis legalization by Diana Tran | BlogsThailand has become the first southeast Asian country to legalize cannabis for medical use. Cannabis was originally introduced into Thailand from India, and until it was outlawed in the 1930s, it was historically used as a kitchen condiment, medicine and source of fiber.
Uploading and using transcriptomics data in the OneOmics suite by Alexandra Antonoplis | Data Visualization, Life Science Research, Multi-Omics, OneOmics, SCIEX informationRNA experiments can be created in the OneOmics suite for multi-omics analyses, enabling integration of transcriptomics data and proteomics data for biological insight. To build RNA experiments, either CloudConnect for PeakView software 2.2 or BaseSpace (Illumina) can...
Downloading results from SCIEX Data Store or BaseSpace by Alexandra Antonoplis | Getting Started, Life Science Research, Multi-Omics, OneOmics, Proteomics, SCIEX informationOnce your data processing sessions have completed, the results files are saved back to either SCIEX Data Store or BaseSpace. These can be downloaded from the cloud to your desktop for additional analysis. Please see these community posts to learn more: Explaining the...
Breaking down the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready by Jianru Stahl-Zeng | Blogs, ClinicalSensitivity and robustness carry different meanings in the world of mass spectrometry. Generally, sensitivity refers to an instrument’s ability to achieve lower limits of detection (LOD). Robustness, on the other hand, refers to an instrument’s ability to consistently...
The honey sting by Katherine Hyland | Blogs, Food / BeverageAs a consumer it’s hard for me not to feel inundated with claims that our food is “all-natural” or “chemical-free” or that we should buy certain “superfoods” for their health benefits. We read labels and trust that the product we are buying is what we are truly...
The top 5 questions to ask when investing in accurate mass technology for forensic toxicology workflows by Holly McCall | Blogs, ForensicAre you considering the purchase of a high-resolution accurate mass (HRAM) instrument for your forensic toxicology lab? To help ensure you invest in a solution that ideally meets your needs, ask yourself the following key questions. 1. How do I ensure my results...
Innovation that’s blasting through limitations in explosive detection by Pierre Negri | Blogs, ForensicMass spectrometry’s important role in identifying explosives The need for rapid explosive detection is now an unfortunate reality. The remit is multifaceted. The first is for preventative purposes, to protect us from any threat to life. The second is in the...
Rapid Characterization of Biologics using CESI-MS by SCIEX Community | Biopharma, BlogsToday, 30 monoclonal antibodies (mAbs), have been approved for the treatment of certain cancers, autoimmune and infectious diseases. Even more are in development, and perhaps you and your team of scientists are working on one now. Keeping pace with fast development timelines while performing comprehensive characterization of biologic candidates can be challenging. However, more and more, scientists are tackling these challenges with new techniques to speed and simplify their characterization workflows. Read more in the application note, “Rapid Characterization of Biologics using a CESI 8000 – SCIEX TripleTOF® System,” found in the Biologics Analytical Characterization Compendium, which highlights how CESI separation coupled with high-resolution mass spectrometry can provide a comprehensive characterization of biotherapeutics.
The Not So Hidden Truth about Climate Change How It’s Poisoning Your Food by Robert Di Lorenzo | Blogs, Food / BeverageDid you know climate change could be poisoning your food? According to the United Nations Environmental Program (UNEP) report on Emerging Issues of Environmental Concern, rising temperatures are making crops more toxic.
Guardians of Antibiotics by SCIEX Community | Blogs, Food / BeverageThis second is a blog series on the global war: Rise of Superbugs! Part 1 took a critical look at the antibiotic threat we face in today’s battlefield. The waning effectiveness of antibiotics as we head into what may seem like a post-antibiotic era has impelled new reformation to at the very least control antibiotic usage to ensure food safety.
Protein Quantitation Workflows using the TripleTOF 6600: A Case Study for Rituximab by SCIEX Community | Biopharma, BlogsAlthough the triple-stage quadrupole (QQQ) mass spectrometer remains the pillar for quantitative LC-MS/MS bioanalytical assays, due in part to the platforms’ high duty cycle when operated in multiple-reaction monitoring (MRM) mode, the applicability of high-resolution mass spectrometry (HRMS) has become of increasing importance for protein quantitation given the complexity of proteolytically digested samples in the surrogate peptide approach. While the QQQ demonstrates high sensitivity and specificity, the relatively low-resolution measurement of m/z may fail to differentiate analyte response from nominally isobaric background interference. In contrast, HRMS with accurate mass assignment of product ion allows interference to be resolved through judicious selection of a post-acquisition mass extraction window whose tolerance is largely dictated by the effective resolution and stability of mass calibration.
Rise of the Super Bugs by SCIEX Community | Blogs, Food / BeverageThe term “antibiotic-free” is becoming more and more popular in food advertising these days. Take Subway for example; in March the company elevated their antibiotic-free policy and introduced a new antibiotic-free rotisserie-style chicken sub, and they plan to, “Nix antibiotics in all its meat by 2025.”
Glyphosate, a Polar Pest Put to Test by SCIEX Community | Blogs, Environmental / Industrial, Food / BeverageNo other pesticide has courted more media attention and controversy in recent months than glyphosate, with governments and national agencies debating its use and health effects.
Using Mass Spec to Detect Trace Explosives by SCIEX Community | Blogs, ForensicThe importance of protecting a country’s border is a very topical issue. The war on drugs and terror is a 24/7 task 366 days a year (2016 is a leap year). The government agencies in charge must be vigilant and maintain instrumentation to prevent terrorism, drug trafficking, and other illegal activities. Mass Spectrometry is rapidly becoming the instrument of choice for border agencies throughout the world when it comes to explosive trace detection and forensic drug compounds.
Routine Food Testing Using Mass Spectrometry by SCIEX Community | Blogs, Food / BeverageThese days, it is not uncommon to hear about the overzealous application of pesticides to crops or the injection of antibiotics into animals. From grocery stores to restaurants, our food is at risk. How then, can consumers be assured that chemical contaminants like these , not to mention the risk of mycotoxin compounds are not making their way to your dinner table?
Industrialize Your Quantitative Proteomics Using a More Simplified Sample Prep by Christie Hunter | Blogsin part 1 and part 2 of this blog series we discussed how you can increase your efficiency for high throughput quantitative proteomics by industrializing your sample analysis and data processing. Microflow SWATH® Acquisition on your TripleTOF® system coupled with OneOmics™ data analysis tools allow you to run samples faster, collect data faster, and process your data files faster. It all adds up to getting more meaningful biological information in a shorter amount of time.
Quantify and Identify Pesticides in Complex Food Samples Using the QTRAP 6500 LC-MS/MS System by SCIEX Community | Blogs, Food / BeverageRecent regulations on food analysis require screening for pesticides using confirmatory techniques, such as GC-MS and LC-MS/MS. More than 1000 pesticides are used worldwide and, along with their metabolites and degradation products, are present in food. There is a demand for powerful and rapid analytical methods that can identify pesticides with high confidence in a broad range of food matrices and quantify at low concentrations with good accuracy and reproducibility. Challenges for pesticide residue laboratories at the moment are the request to test for more compounds, in a wider range of samples, all without sacrificing data quality.
Industrialize Your Quantitative Proteomics with the OneOmics Project by Christie Hunter | Blogs, Life Science Research, ProteomicsFor many labs, the days are long gone when it was acceptable to run only a few samples a week for your quantitative proteomics projects. The pressure for faster turn-around times, to support larger cohort studies, to sustain multiple research directions, and to transition from a purely unbiased discovery mode to verifying something truly unique and interesting, all demand a faster pace. Many labs are now being asked to analyze a hundred samples a week or more. In part 1 of this blog series, we saw how moving to a microflow SWATH workflow can dramatically increase your throughput with little compromise on overall results. In this part, we’ll address what to do with all of this data because it’s just no good if all we’ve done is move the bottleneck downstream.
Fast, Efficient, Disulfide Bond Mapping Using BioPharmaView™ Software by SCIEX Community | Biopharma, BlogsFast LC-MS acquisition and automated data processing will help you speed up peptide mapping of your biotherapeutic, including critical disulfide bond and post-translational modification characterization. SCIEX helps you untangle the complexity of disulfide bonds, speeding up your characterization process.
Bottom-Up Proteomics: A Discussion with Christie Hunter by Christie Hunter | Blogs, Life Science Research, ProteomicsBiocompare recently featured an article on Bottom-Up Proteomics. I had a chance to follow up with Christie Hunter and expand on some of the questions featured in the article:
Characterize and Monitor Host Cell Proteins (HCPs) Using SWATH Acquisition Technology by SCIEX Community | Biopharma, BlogsDuring drug development, the removal of impurities and purification of a final drug product is absolutely essential in order to ensure the safety and efficacy of a therapeutic drug. Of particular concern for biologics are impurities that can stem from host cell proteins. Because biologics are developed through cell culture and fermentation within a host cell, proteins from this host cell can be co-purified with the final biologic. These host cell proteins or HCPs can cause the final product to have undesired side-effects such as eliciting an immune response in patients taking the drug, or affecting the drug’s stability or efficacy. As a result, regulating agencies require drug companies to monitor levels of HCPs during the development and purification of a biologic and to remove HCPs to an acceptable level in the final biotherapeutic product.
Simplifying Biologics Bioanalysis Sample Prep by SCIEX Community | Biopharma, BlogsThese days, everyone seems to be furiously scratching tickets to become instant winners, but I’ll bet you didn’t expect to find sample prep tips that way. For large molecule bioanalysis, preparing your samples can be one of the biggest challenges. It’s a whole different world from traditional small molecule bioanalysis. SCIEX has developed techniques and automation that make biologics sample prep simpler and faster, with reproducible results.
Taking on Precision Medicine with Industrialized Proteomics by Neil Walsh | Blogs, Life Science Research, OneOmics, ProteomicsWhat if we could deliver the right treatment at the right time, to the right person to better, more effectively treat complex disease? This is the promise of precision medicine, to be able to approach complex disease treatment and prevention by taking into account individual variability in genes, environment, and lifestyle for each person.
The Connection Between Mass Spectrometry and Space Exploration by SCIEX Community | Blogs, TechnologyMass spectrometry has been used for some pretty fascinating applications in our world – like testing for steroid use in athletes1, measuring pesticides in grapes2, assessing the efficiency of a psoriasis drug3, and whether that expensive bottle of 100% olive oil is, well, really 100% olive oil.4 But did you know mass spec is also used out of this world? Like… in space?
The Future of Biologics Drug Development is Today by SCIEX Community | Biopharma, BlogsSince the 1982 approval of Eli Lilly’s recombinant human insulin, Humulin, biotherapeutic drug development has steadily grown into a global market valued at $140 billion in 2013, increased from $25 billion in 2001
A Hybrid LBA/LC-MS Assay – Your Questions Answered by SCIEX Community | Biopharma, BlogsLast week we posted a blog on Biologics Bioanalysis Key Challenges, where we presented a webinar on those key challenges.
Using Mass Spectrometry to Identify and Quantify Contaminants in Water Samples by SCIEX Community | Blogs, Environmental / Industrial, Food / BeverageAccess to clean wholesome water is one of our basic human rights. Human engineering has designed incredible methods to collect, filter, purify, store and distribute water to billions of us worldwide, but does this mean that our water is completely safe to drink? Also,...