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Guide decisions during cell line development with more information at the intact level

Guide decisions during cell line development with more information at the intact level

Monitoring product quality attributes (PQAs) throughout monoclonal antibody (mAb) development is vital to ensuring drug safety and efficacy. By adopting orthogonal analytical techniques and integrating new technologies that have the potential to provide more information, it is possible to improve product quality and manufacturing efficiency and make more informed decisions.

What has the Echo® MS system done for the pharma industry? (And don’t just take our word for it!)

What has the Echo® MS system done for the pharma industry? (And don’t just take our word for it!)

SCIEX was very proud to have an illustration of the Acoustic Ejection Mass Spectrometry (AEMS) technology that powers the Echo® MS system on the front cover of the Journal of the American Society for Mass Spectrometry in January 2023. The associated article—Ultrahigh-Throughput Intact Protein Analysis with Acoustic Ejection Mass Spectrometry—was co-authored by scientists from SCIEX and Merck.

How do I define the experimental design (the metadata) for my SWATH acquisition study within the OneOmics suite? What are the requirement for replicates?

How do I define the experimental design (the metadata) for my SWATH acquisition study within the OneOmics suite? What are the requirement for replicates?

In quantitative Omics research, the goal is to understand which analytes (protein or metabolite) are perturbed between experimental conditions; therefore we carefully design our studies to explore these questions. The algorithms used within the Assembler application...

Multi-Laboratory Study Highlights the Quantitative Reproducibility of SWATH Acquisition (Nature Communications Paper)

Multi-Laboratory Study Highlights the Quantitative Reproducibility of SWATH Acquisition (Nature Communications Paper)

Reproducibility is one of the key tenets of the scientific method. But in a recent survey published in Nature, more than 70% of researchers were not able to reproduce another scientist’s experiments, and more than half could not reproduce their own experiments1. While the reasons for this are many, at least some of them stem from issues inherent in data collection.

SCIEX Lands HUPO Science and Technology Award

SCIEX Lands HUPO Science and Technology Award

We are pleased to congratulate its research scientists Stephen Tate and Ron Bonner (retired) for being awarded this year’s Science and Technology award at HUPO 2017 in Dublin Ireland. The Science and Technology Award at HUPO recognizes an individual or team who were key in the commercialization of a technology, product, or procedure that advances proteomics research

Happy Birthday to SWATH Acquisition! 5 Years of Innovation

Happy Birthday to SWATH Acquisition! 5 Years of Innovation

With its introduction at the HUPO World Congress in 2010 in Sydney Australia by Ruedi Aebersold, SWATH® Acquisition instantly intrigued scientists around the world. Here was a new technique with the potential to revolutionize the way proteomics studies were performed! Based on a data independent acquisition strategy using a SCIEX TripleTOF® 5600 system, SWATH was able to consistently identify and quantify at least as many peptides and proteins as other far more mature proteomics strategies on the market, but with quantitative accuracy and reproducibility rivaling gold standard MRM experiments! This solution was made broadly available to researchers with a full launch of SWATH Acquisition in the Analyst® TF 1.6 Software on the TripleTOF 5600+ System at ASMS 2012 in Vancouver (A Mine of Quantitative Proteomic Information.  Prof Dr. Ruedi Aebersold, Head of the Department of Biology, ETH Zurich).

5 Tips for Calibrating a QTOF Mass Spectrometer

5 Tips for Calibrating a QTOF Mass Spectrometer

Do you have questions about your mass spec? How about a workflow? Our community members are involved in active discussions and receive expert answers from customers like you, SCIEX scientists, and support specialists every week. One recent topic concerned the automatic calibration on TripleTOF® systems as answered by Dr. Christie Hunter whose focus is developing and testing innovative MS workflows for omics research through working collaboratively with the instrument, chemistry, and software research groups.

Discover The Benefits of Knowledge Base Articles

Discover The Benefits of Knowledge Base Articles

Did you know you can access Knowledge Base Articles for trending user questions compiled and answered by SCIEX support experts? Doing so may help to reduce your support calls, not to mention downtime. Instead of waiting for a problem to occur, you can stay on top of it, and be a part of the solution. To give you an idea of trending articles, consider the how this past month saw questions and answers including:

Data Independent Acquisition Mass Spectrometry with the Power of SWATH

Data Independent Acquisition Mass Spectrometry with the Power of SWATH

There are many different methods in use today to acquire data on a mass spectrometer, but few have generated as much buzz in recent years as SWATH technology. First reported 5 years ago by Ruedi Aebersold and his group1, SWATH® Acquisition on a TripleTOF® instrument has rapidly become one of the premier acquisition strategies for identification and quantitation of complex samples. But what exactly is SWATH and why is it so powerful? In order to answer these questions, let’s first take a step back and look at the larger picture.

Vice President Biden Announces Agreement Naming Children’s Medical Research Institute’s ProCan Lab to the ‘Cancer Moonshot’ Initiative

Vice President Biden Announces Agreement Naming Children’s Medical Research Institute’s ProCan Lab to the ‘Cancer Moonshot’ Initiative

A key goal of the ‘Cancer Moonshot’ initiative is the advancement of precision medicine, with the goal of making more targeted therapies available to more cancer patients. And researchers believe that the time is right, with the new technological innovations, the new insight into the biology of cancer and big improvements in the handling of ‘big data.’

Stoller Biomarker Discovery Centre, Addressing Some of the Biggest Issues in Medicine

Stoller Biomarker Discovery Centre, Addressing Some of the Biggest Issues in Medicine

The Stoller Biomarker Discovery Center, developed in partnership with SCIEX, was created to develop new omics technologies for biomarker research to understand the root cause of diseases such as cancer, cardiovascular disease, and autoimmune diseases. We initially announced our collaboration with the University of Manchester back in October 2015. 

The History of Isotopic Labels for Quantitative Proteomics

The History of Isotopic Labels for Quantitative Proteomics

Proteomics has become a vital tool for biological scientists performing research on the healthy and diseased states of living things. It involves the large scale and systematic analysis of all proteins within a given cell, tissue, or organism. Because proteins are regulated by many different internal and external stimuli, the proteome is dynamic and quantities of proteins can change from one state to the next. Therefore, in order to be of the highest utility, proteomics experiments need to both identify and quantify proteins so that comparative studies can be done, such as between healthy cells and tumor cells, or the comparison of different treatment regimens.

The Promise of Precision Medicine

Here is the latest update on the Worldwide Efforts to Accelerate Precision Medicine

The NIH recently issued a press release in early July announcing $55 million in awards. According to the release, the $55 million award in the fiscal year 2016 will go towards building the foundational partnerships and infrastructure needed to launch the Cohort Program of President Obama’s Precision Medicine Initiative (PMI). The PMI Cohort Program is a landmark longitudinal research effort that aims to engage 1 million or more U.S. participants to improve the ability to prevent and treat disease based on individual differences in lifestyle, environment, and genetics.

Why Study Lipids?

Why Study Lipids?

I had an opportunity to follow up with Steven M Watkins, Ph.D. to talk about the importance of studying lipids in disease. Steve has been working in the lipids field for over 20 years and is one of the foremost experts in lipid biology. Steve founded Lipomics in 2000, an early metabolomics company focused on quantitative lipidomics and had followed that company through a series of changes that led to its involvement in the clinical diagnostic development and global metabolomics. Steve authored over 70 peer-reviewed publications including several book chapters on lipids and lipid metabolism. His presentations on this topic are fascinating and very informative, so I wanted to capture some of his thinking here!

Improved complex sample processing for higher quality of results, reproducibility and depth of proteomic analysis

Improved complex sample processing for higher quality of results, reproducibility and depth of proteomic analysis

SCIEX partners to improve depth of proteome coverage
SCIEX and Pressure BioSciences address a major challenge for researchers performing complex sample preparation by marketing a complete solution to increase the depth, breadth, and reproducibility of protein extraction, digestion, and quantitation in all tissue types, especially challenging samples like tumors.

Industrialize Your Quantitative Proteomics Using a More Simplified Sample Prep

Industrialize Your Quantitative Proteomics Using a More Simplified Sample Prep

in part 1 and part 2 of this blog series we discussed how you can increase your efficiency for high throughput quantitative proteomics by industrializing your sample analysis and data processing. Microflow SWATH® Acquisition on your TripleTOF® system coupled with OneOmics™ data analysis tools allow you to run samples faster, collect data faster, and process your data files faster. It all adds up to getting more meaningful biological information in a shorter amount of time.

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