Search
Page 54 of 61
Harnessing the Power of MRM3 for Large Molecule Quantitative Bioanalysis
In a previous blog outlining the advantages of high-resolution accurate mass measurements for protein quantitation using the TripleTOF 6600, it was noted that although the triple-stage quadrupole demonstrated high sensitivity when operated in multiple reaction monitoring mode (MRM), the relatively low-resolution measurement of m/z failed to discriminate Rituximab response from nominally isobaric interferences given the complexity of the proteolytically digested samples (June 28/2016). While the accurate mass filtering capabilities of the TripleTOF 6600 represents one mechanism for achieving increased selectivity over MRM, the triple quadrupole/linear ion trap (LIT) hybrid platform represented by the QTRAP® 4500, 5500, 6500 and 6500+ systems provides an alternative technique by leveraging a third stage of MS, often referred to as MRM3. In this blog, we outline the MRM3 scan function and survey several large molecule applications which utilize the additional stage of fragmentation in the LIT to yield significant improvements in achievable detection limits when compared to MRM.
Tags: Biopharma, biotherapeutic development, blaze, charge, company, couple, development, job, machine, mass, money, new, number, power, protein, rapid, system, technology, time, variant, work
October 4, 2016
Taking care of your mass spectrometer—Onsite troubleshooting and maintenance training for today’s lab
Recently, we asked customers to tell us about their biggest challenges so we could customize training programs to meet the needs of today’s growing lab. Without hesitation, most of you said uptime and employee training are your most critical needs. As a result, our SCIEX Now Learning Hub educational support team has been hard at […]
Tags: application, company, computer, different, form, guide, information, ion, job, machine, mass, method, option, research, science, sensitivity, system, time, triple, work
September 26, 2016
A Sting in the Tale for Neonicotinoids
Did you know one out of every three mouthfuls of your meal is a product of honeybee pollination—almonds and other tree nuts, berries, fruits, vegetables? To put numbers behind it, honeybee pollination amounts to about $15 billion of U.S. agriculture annually through increased yields and superior quality harvests. So why are we buzzing about bees? Well, since 2006, beekeepers […]
Tags: biotherapeutic development, blaze, charge, company, couple, development, Food / Beverage, job, machine, mass, money, new, number, power, protein, rapid, system, technology, time, variant, work
The detection of acid herbicides and urons by large volume injection
Pre-treatment versus direct injection – that is the question posed in the application note, “The Detection of Acidic Herbicides and Phenyl Ureas by LC-MS/MS with Large Volume Injection and Automated Column Switching.” It’s just one of the dozens of articles you will find within the Environmental Compendium (pages 1 to 4, pesticides) now available for download.
Tags: biotherapeutic development, blaze, charge, company, couple, development, Environmental, job, machine, mass, money, new, number, power, protein, rapid, system, technology, time, variant, work
See What More You Can do With 360 Degree Biologics Characterization
Ever wish you had your own team of mass spectrometry experts at your side when working through biologics development and characterization challenges? With SCIEX 360° Innovations complex biologics characterization is streamlined with a full suite of mass spectrometry (MS), capillary electrophoresis (CE) systems, software, and services from SCIEX experts.
Tags: Biopharma, biotherapeutic development, blaze, charge, company, couple, development, job, machine, mass, money, new, number, power, protein, rapid, system, technology, time, variant, work
September 14, 2016
The Promise of Precision Medicine
Here is the latest update on the Worldwide Efforts to Accelerate Precision Medicine
The NIH recently issued a press release in early July announcing $55 million in awards. According to the release, the $55 million award in the fiscal year 2016 will go towards building the foundational partnerships and infrastructure needed to launch the Cohort Program of President Obama’s Precision Medicine Initiative (PMI). The PMI Cohort Program is a landmark longitudinal research effort that aims to engage 1 million or more U.S. participants to improve the ability to prevent and treat disease based on individual differences in lifestyle, environment, and genetics.
Tags: application, company, computer, different, form, guide, information, ion, job, machine, mass, method, option, Proteomics, research, science, sensitivity, system, time, triple, work
August 26, 2016
You’ve Seen It… Now Try It! BioPharmaView Software 2.0
At ASMS this year, the newest version of BioPharmaView Software was released. This software simplifies the processing of biotherapeutic data for characterization and comparability which can dramatically improve your productivity. BioPharmaView 2.0 Software accelerates characterization and comparability studies and simplifies reporting, so you can make better decisions, faster.
Tags: Biopharma, biotherapeutic development, blaze, charge, company, couple, development, job, machine, mass, money, new, number, power, protein, rapid, system, technology, time, variant, work
August 23, 2016
Quantitation of Antibiotics and Insecticides in Poultry Feed using LC-MS/MS
Quantitating antibiotics and insecticides in poultry is serious business. Overuse can lead to antibiotic resistance while insecticide residuals can cause harmful side effects in humans. In the United States, for example, the Federal Drug Administration (FDA), has offered up a plan to limit common antibiotics in feed, which are used to encourage growth. However, this is a voluntary plan, and as the following application note, “Quantitation of Antibiotics and Insecticides in Poultry Feed using LC-MS/MS,” points out, antibiotics have been shown to accumulate in poultry feathers, which are in turn used for nutritional elements in the feed.
Tags: biotherapeutic development, blaze, charge, company, couple, development, Food / Beverage, job, machine, mass, money, new, number, power, protein, rapid, system, technology, time, variant, work
August 22, 2016
Screening Novel Psychoactive Substances with Confidence
How do you know what you can’t see? This is the challenge many a lab faces as they relentlessly test for novel psychoactive substances (NPS) as unknown samples with an ever-changing ingredient list make discovery difficult work at best. There are many reasons for the complexities of which you can discover in this application note, “Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances.” However, the biggest of which is that non-targeted findings can turn up thousands of molecular features in a single sample. Sifting through the peaks is laborious, and many are normal besides.
Tags: biotherapeutic development, blaze, charge, company, couple, development, job, machine, mass, money, new, number, power, protein, rapid, system, technology, time, variant, work
August 10, 2016
Why Study Lipids?
I had an opportunity to follow up with Steven M Watkins, Ph.D. to talk about the importance of studying lipids in disease. Steve has been working in the lipids field for over 20 years and is one of the foremost experts in lipid biology. Steve founded Lipomics in 2000, an early metabolomics company focused on quantitative lipidomics and had followed that company through a series of changes that led to its involvement in the clinical diagnostic development and global metabolomics. Steve authored over 70 peer-reviewed publications including several book chapters on lipids and lipid metabolism. His presentations on this topic are fascinating and very informative, so I wanted to capture some of his thinking here!
Tags: application, company, computer, different, form, guide, information, ion, job, Lipidomics, machine, mass, method, option, research, science, sensitivity, system, time, triple, work
July 28, 2016
Page 54 of 61