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There’s no doubt that mass spectrometry has emerged as an essential technique for drug discovery and development. But the denaturing conditions of conventional LC-MS will typically break apart any non-covalent interactions that exist in solution – even though there is...

From small ions like phosphate, herbicide degradation to metabolites, oligosaccharides, peptides, and proteins. How is your lab analyzing polar molecules? The reason I ask is there is a saying, if you have a charged or polar molecule, look to capillary electrophoresis (CE) first. While liquid chromatography (LC) is an ideal front-end separation tool for many types of molecules, as the following poster points out, “From Small to Very Large, Orthogonal, Sensitive Polar Molecule Analysis by CESI-MS,” there are some situations that call for CE over LC analysis. For those of you that are not familiar with CESI-MS, it is the combining of CE separation with electrospray ionization, into one dynamic process, within the same device.

Quantitation of monoclonal antibodies (mAbs) in biological fluids is important during all stages of antibody drug development. First developed in the 1970s, therapeutic mAbs have both research and medicinal impact as they can be used for diagnosis and treatment of a wide variety of diseases, and have a high level of specificity.

In an effort to Replace, Refine, and Reduce the number of animals used for pre-clinical research, several microsampling strategies have been implemented which allow for the consolidation of satellite TK and main study groups. In addition to the ethical gains driven by these 3Rs, microsampling has the potential of increasing scientific value since it becomes feasible to directly correlate exposure, toxicological effects and pharmacological response in the same individual

Is your mind swimming with large molecule catabolism data? Do you spend hours manually processing through complex spreadsheets to match your data with theoretical biologic catabolites? Are you wasting precious time by drawing out and processing your therapeutic peptide as a small molecule? Are you worried that you could be missing something?

There is a lot of talk going around in the lab, and it has to do with the newly released Fast Glycan Labeling and Analysis technology. Where once research analysts needed to set aside days to perform glycan analysis, now takes an hour or so. Glycans are immediately identified by the software – so no need for data interpretation. That’s up to 5x faster than HILIC.

Did you know the X500B QTOF system makes point and click workflows for Biologics Characterization possible on your mass spectrometer? The newly-designed SCIEX OS software interface brings to life fluid navigation and ease of use so you can keep moving forward on your scientific discoveries. In fact, it’s so simple to learn and operate that you and your team can be up and running faster than you might expect.

Learning a new mass spec system can be a daunting task. Aside from the opportunity costs of training new users, you might face the hassle of downtime, and the wait to get expert help when needed. The X500B QTOF system puts a new spin on biologics characterization workflows because it is so easy to learn and operate that you can be up and running much faster than you expect. Powerful new software tools dramatically streamline method development and data processing, to enable everyone in your lab to get expert results. It’s fast because it’s easy, even for new users.

Have you ever wished for a compact instrument that delivers expert-level answers to your most complex biotherapeutic characterization challenges faster and easier than what you are doing now? At SCIEX, we recognize that even expert users want easier ways to perform daily characterization tasks and get great results every time. That’s why we set out to develop the X500B QTOF system: a robust and reliable new instrument and software solution that reduces complexity and simplifies biologics characterization workflows so every scientist can get expert-level results

Protein-based biotherapeutics, including monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) are a growing component of pharmaceutical companies’ drug pipelines. The growth of ADCs in particular is due to their ability to selectivity target and deliver a potent molecule to a cancer cell based on a specific tumor marker. In order to support this growing class of new drug molecules, robust and reliable bioanalytical methods are required. While ligand binding assays (LBAs) like ELISA have been the most popular platform for biotherapeutic quantitation, bioanalytical scientists have been increasingly adopting hybrid LBA/LC-MS methods in this area.

Throughout all stages of development and manufacture, monoclonal antibodies (mAbs) exhibit a great deal of structural complexity. After translation and folding, proteins undergo post-translational modifications, as well as spontaneous and enzymatic degradation, such that a single preparation of purified mAb exhibits a range of small structural changes, composed of various glycoforms and charge variants, as well as amino acids alterations due to oxidation, deamidation, isomerization, or other chemical reactions. This display of structural heterogeneity can influence the overall stability, efficacy, and safety profile; therefore, understanding the extent of structural modifications has become extremely important to drug manufacturers who continually assess mAb composition throughout bioprocessing to demonstrate stability, batch-to-batch consistency, and long-term shelf life.

A variety of post-translational modifications (PTMs) can impact a biotherapeutic protein’s mass, but none are as common as glycosylation.[1] Hence, the headline for a recent article in Genetic Engineering and Biotechnology News,  “Post-Translational Icing on the Biologics Cake,” featuring comments from Sean McCarthy, Ph.D., Global Market Manager of Biologics at SCIEX.

SelexION® DMS Technology Drives Advancements in Challenging Large Molecule Bioanalysis By the End of 2024, the peptide therapeutics market value is expected to reach US$46.6 billion1. However, peptide therapeutics present some of the toughest analytical...

In a previous blog outlining the advantages of high-resolution accurate mass measurements for protein quantitation using the TripleTOF 6600, it was noted that although the triple-stage quadrupole demonstrated high sensitivity when operated in multiple reaction monitoring mode (MRM), the relatively low-resolution measurement of m/z failed to discriminate Rituximab response from nominally isobaric interferences given the complexity of the proteolytically digested samples (June 28/2016). While the accurate mass filtering capabilities of the TripleTOF 6600 represents one mechanism for achieving increased selectivity over MRM, the triple quadrupole/linear ion trap (LIT) hybrid platform represented by the QTRAP® 4500, 5500, 6500 and 6500+ systems provides an alternative technique by leveraging a third stage of MS, often referred to as MRM3. In this blog, we outline the MRM3 scan function and survey several large molecule applications which utilize the additional stage of fragmentation in the LIT to yield significant improvements in achievable detection limits when compared to MRM.

Ever wish you had your own team of mass spectrometry experts at your side when working through biologics development and characterization challenges?  With SCIEX 360° Innovations complex biologics characterization is streamlined with a full suite of mass spectrometry (MS), capillary electrophoresis (CE) systems, software, and services from SCIEX experts.

At ASMS this year, the newest version of BioPharmaView Software was released. This software simplifies the processing of biotherapeutic data for characterization and comparability which can dramatically improve your productivity. BioPharmaView 2.0 Software accelerates characterization and comparability studies and simplifies reporting, so you can make better decisions, faster.

The move to large molecules in Pharma is accelerating, offering unprecedented opportunities to improve human health and expand into new markets. But for those with extensive experience with small molecule bioanalysis, the shift to biologics can be challenging, from Sample Prep to Instrumentation and Software, to Methods and Training:

Today, 30 monoclonal antibodies (mAbs), have been approved for the treatment of certain cancers, autoimmune and infectious diseases. Even more are in development, and perhaps you and your team of scientists are working on one now.  Keeping pace with fast development timelines while performing comprehensive characterization of biologic candidates can be challenging. However, more and more, scientists are tackling these challenges with new techniques to speed and simplify their characterization workflows. Read more in the application note, “Rapid Characterization of Biologics using a CESI 8000 – SCIEX TripleTOF® System,” found in the Biologics Analytical Characterization Compendium, which highlights how CESI separation coupled with high-resolution mass spectrometry can provide a comprehensive characterization of biotherapeutics.

Although the triple-stage quadrupole (QQQ) mass spectrometer remains the pillar for quantitative LC-MS/MS bioanalytical assays, due in part to the platforms’ high duty cycle when operated in multiple-reaction monitoring (MRM) mode, the applicability of high-resolution mass spectrometry (HRMS) has become of increasing importance for protein quantitation given the complexity of proteolytically digested samples in the surrogate peptide approach.  While the QQQ demonstrates high sensitivity and specificity, the relatively low-resolution measurement of m/z may fail to differentiate analyte response from nominally isobaric background interference.  In contrast, HRMS with accurate mass assignment of product ion allows interference to be resolved through judicious selection of a post-acquisition mass extraction window whose tolerance is largely dictated by the effective resolution and stability of mass calibration.

Fast LC-MS acquisition and automated data processing will help you speed up peptide mapping of your biotherapeutic, including critical disulfide bond and post-translational modification characterization. SCIEX helps you untangle the complexity of disulfide bonds, speeding up your characterization process.