Answer: When it’s a QTRAP® In today’s modern and frenzied analytical laboratories, triple quadrupole mass spectrometers (MS) are the ‘workhorse,’ frontline instrument for carrying out quantitative assays from drug discovery to food safety. To get the best selectivity...
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An overview: LC-MS analysis of targeted protein degraders and their metabolites
Targeted protein degraders (TPD) are a relatively new therapeutic modality that opens the potential to target disease-causing proteins. These disease-causing proteins have been highly challenging for traditional small-molecule therapeutics to treat, making TPDs an exciting new therapeutic modality.
Guide decisions during cell line development with more information at the intact level
Monitoring product quality attributes (PQAs) throughout monoclonal antibody (mAb) development is vital to ensuring drug safety and efficacy. By adopting orthogonal analytical techniques and integrating new technologies that have the potential to provide more information, it is possible to improve product quality and manufacturing efficiency and make more informed decisions.
Processing ZenoTOF 7600 system data with DIA-NN software
DIA-NN software is a powerful software tool for processing data independent acquisition (DIA) proteomics datasets. It uses neural networks and other algorithms to identify and quantify peptides and proteins from DIA data and is specifically optimized for fast...
Meat vs plant based. What is the best option?
As we become more conscious about the planet, healthier lifestyles and our duty to protect the environment, attitudes and behaviours are shifting when it comes to food consumption.
How is the protein level fold-change computed on proteomics data using Assembler app?
With bottom-up proteomics, we quantify peptides in the sample and then infer the protein level quantitation from that data. However, due to post-translational modifications, different protein isoforms, etc. we don’t always expect that all peptides associated with the...
Computing protein confidence with improved accuracy by reassessing peptide confidence during protein grouping
ProteinPilot software 4.0 and higher releases include a new method for calculating protein confidences that improves reliability at the end of protein lists. Figure 1 shows a simulated example that demonstrates how more accurate protein confidence is computed. Figure...
Intabio acquisition expands SCIEX portfolio of impactful solutions to accelerate biotherapeutic development
Due to the nature of their production, biotherapeutics are difficult to manufacture. Growth conditions, purification protocols and formulation requirements can introduce unintended modifications into the protein structure that may affect its efficacy and safety.
Breaking down the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready
Sensitivity and robustness carry different meanings in the world of mass spectrometry. Generally, sensitivity refers to an instrument’s ability to achieve lower limits of detection (LOD). Robustness, on the other hand, refers to an instrument’s ability to consistently...
The honey sting
As a consumer it’s hard for me not to feel inundated with claims that our food is “all-natural” or “chemical-free” or that we should buy certain “superfoods” for their health benefits. We read labels and trust that the product we are buying is what we are truly...
Innovation that’s blasting through limitations in explosive detection
Mass spectrometry’s important role in identifying explosives The need for rapid explosive detection is now an unfortunate reality. The remit is multifaceted. The first is for preventative purposes, to protect us from any threat to life. The second is in the...
Don’t Be a Food Scandal Scapegoat. Fight Back with LC-MS/MS for Your Meat Authenticity Testing
The infamous horsemeat scandal of 2013 shined a spotlight on the questionable integrity of our meat supply. What followed was a lengthy investigation as the extent of the scandal unraveled before our eyes, reaching far beyond the European borders...
It’s Time to Turn Things Around―Faster and Accurate Mass Analysis of Organic Explosives
Sadly, the world is a volatile place. The threat of organic explosive use by terrorists and criminals is very much with us, posing a significant danger to people worldwide. The rising concern for public safety is a top priority for national security organizations...
Push Your Research to the Cutting Edge: 2018 Global CESI-MS Symposium
There are a lot of conferences vying for your attention every year. The Global CESI-MS Symposium on October 10-11 in the Netherlands is one you can’t afford to miss. It is the place to hear about the latest advancements made through the adoption of CESI-MS...
Break the routine: SCIEX OS-MQ improves your quantitation workflows
Advances in today’s technology have resulted in a need for higher and faster sample throughput, with increasing number of measured analytes per sample. The new SCIEX OS-MQ software breaks the routine and takes quantitation workflows to a new level. Key features...
Stop Fumbling Around in the Dark: Try LC-MS/MS for Designer Drugs in Forensic Samples
Imagine being blindfolded, sent into a large (dark) room filled with obstacles and challenged to find an item, but you don’t know what that item is, and you have never seen it before. Then you must do the same again the next day, but you are looking for a different...
Something’s in the Water: Tackle Your PFAS Analysis
Exposure to per- and polyfluoroalkyl substances (PFAS) residues could be dangerous. These chemicals have been linked to a variety of adverse health effects including liver damage, thyroid disease, decreased fertility, high cholesterol, obesity, hormone suppression,...
5 Reasons Why Mass Spec is Transforming Clinical Labs
According to the American Clinical Laboratory Association, more than 7 billion clinical lab tests are performed in the U.S. every year. While mass spectrometry represents only a fraction of the clinical applications, there are a growing number of tests where mass...
Save Effort. Save Time. Consolidate Your BioTherapeutic Quality, Safety, and Efficacy Assessments with One Direct LC-MS MAM Assay.
In a previous blog, we learned how you could Simplify Your Life with a Streamlined Workflow for Multiple Attribute Methodology (MAM). Based on the well-known technique of peptide mapping, a Liquid Chromatography-Mass Spectrometry-based MAM workflow (LC-MS MAM) enables...
Metabolism and Biotransformation – What Are You Missing?
Are you looking to improve your MetID studies? Want to achieve the productivity and throughput you need to keep your projects moving and have complete confidence that you’re not missing any low level or parent-like metabolites in your sample? In this blog, we’re...
Biologics Characterization: Intact and Subunit Mass Analysis
When All You Need Is Range – Mass Range, Dynamic Range, and the Complete Range Launching the best possible product in the shortest time possible is key for pharmaceutical companies like you. As you know, nearly every process throughout the biologics development cycle...
“Bottoms Up” Proteomics
Ahhhh beer. It's a ubiquitous drink found in over 90% of all countries around the world. Since the dawn of civilization, man has celebrated with beer where it can make even the most introverted person suddenly dance a little jig or belt out a top 40 song. But other...
Now You Can Overcome Your Food and Environmental Testing Obstacles
How do you safeguard human health and protect your reputation through meeting stringent global food and environmental safety standards? When it comes to testing food and environmental samples, the quality of your testing data is vital to your business. You need fast...
Unraveling the Metabolome
Dr. David Wishart, Departments of Biological Sciences and Computing Science, University of AlbertaCanada’s TMIC is an internationally-recognized leader in metabolomics, providing tools and resources that are used by laboratories around the world. The center offers...
It’s Time to Stop Fraudulent Food Reaching the Consumer with LC-MS/MS
If you are reading this blog, it is likely that you are fully acquainted with food fraud, but let’s just take a moment to set the scene. Food fraud is by no means a new phenomenon and goes as far back as the middle ages, with the concept of tampering or...
Product Quality Assessments of Biotherapeutics Using LC-MS MAM
Why LC-MS MAM?The Liquid Chromatography-Mass Spectrometry Multiple Attribute Methodology (LC-MS MAM) is a technique that is quickly gaining traction in the development and manufacturing of biopharmaceuticals. But what exactly is MAM? As researchers describe in a...
Ion Formation Control Is the Key to Increasing System Robustness
Traditional mass spectrometry ionization methods such as electronic spray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI), are popular methods for detecting the molecular weight of proteins, peptides, and other biologics. The reason being both...
Why You Need Mass Spec for Veterinary Drug Residue and Antibiotic Analysis
As hospitals struggle with increasingly hard-to-treat antimicrobial resistance (ARM) bacterial infections, related deaths now exceed 700,000 per year globally and are predicted to reach 10 million per year by 2050. There’s no denying that these statistics are both...
Simplify Your Life with a Streamlined Workflow for Multiple Attribute Methodology (MAM)
The effort to fully characterize and release a biotherapeutic to the market can be onerous. Typically, many tests are required to identify and monitor various attributes of the final product in order to ensure the safety and efficacy of the drug. These product quality...
Solving the Mystery of Forensic Quantitative Analysis
Why You Need Differential Mobility Spectrometry for Forensic Mass Spec Analysis Forensics depend on detection of even the smallest compounds to deliver results you can rely on. You need fast analysis methods that provide highly accurate data across a multitude of...
Calling all Bioanalysts: We’re Making Bioanalytical Selectivity Challenges History
High selectivity is a key component of successful quantitative bioanalysis. As a bioanalyst, we need consistently accurate and robust quantitation of small molecule therapeutics and metabolites. Challenged by complicated matrix interferences, high baseline signal, and...