Detecting low levels of drugs and their metabolites in hair and nail samples using LC-MS/MS by SCIEX Community | 0 CommentsYou probably have heard of testing blood and urine samples for the presence of drugs and their metabolites. But do you know about the benefits of hair and nail analysis? In a recent webinar, Tina Binz, Deputy Head of the Center for Forensic Hair Analysis, University of Zurich, discussed the benefits of developing comprehensive and sensitive LC-MS/MS for the detection of low-level drugs and metabolites in keratinized matrices.
LC-MS system replacement: Are you ready? by Kirsten Craven | 0 CommentsMeeting deadlines in a bioanalysis laboratory can be a big challenge. Older, less sensitive and less reliable LC-MS systems make it even more difficult. Even the disruption caused by the installation and validation can be disconcerting and delay decisions. Does this sound familiar?
Questions and answers to help improve your mycotoxin analysis by Michael Zellmann | 0 CommentsDuring a recent webinar I shared method details for mycotoxin analysis on the SCIEX 7500 system. In this blog i will share the Q&A for the submitted questions that we did not have chance to answer during the live webinar.
Maximize NPS analysis with accurate mass spectrometry by Pierre Negri | 0 CommentsLC-MS/MS is a powerful analytical tool in forensic toxicology testing that can support a variety of testing regimes such as screening, confirmation and quantitative workflows. More specifically, analysis of NPS using LC-MS/MS provides many advantages, including the ability to reliably detect new drugs and their metabolites from a variety of biological matrices.
Detecting low levels of drugs and their metabolites in hair and nail samples using LC-MS/MS by SCIEX Community | Blogs, Forensic, ToxicologyYou probably have heard of testing blood and urine samples for the presence of drugs and their metabolites. But do you know about the benefits of hair and nail analysis? In a recent webinar, Tina Binz, Deputy Head of the Center for Forensic Hair Analysis, University of Zurich, discussed the benefits of developing comprehensive and sensitive LC-MS/MS for the detection of low-level drugs and metabolites in keratinized matrices.
LC-MS system replacement: Are you ready? by Kirsten Craven | Blogs, Pharma, QTRAP / Triple QuadMeeting deadlines in a bioanalysis laboratory can be a big challenge. Older, less sensitive and less reliable LC-MS systems make it even more difficult. Even the disruption caused by the installation and validation can be disconcerting and delay decisions. Does this sound familiar?
Questions and answers to help improve your mycotoxin analysis by Michael Zellmann | Blogs, Food / Beverage, Food and BeverageDuring a recent webinar I shared method details for mycotoxin analysis on the SCIEX 7500 system. In this blog i will share the Q&A for the submitted questions that we did not have chance to answer during the live webinar.
Maximize NPS analysis with accurate mass spectrometry by Pierre Negri | Blogs, Forensic, SCIEX OS software, Toxicology, ZenoTOF 7600 systemLC-MS/MS is a powerful analytical tool in forensic toxicology testing that can support a variety of testing regimes such as screening, confirmation and quantitative workflows. More specifically, analysis of NPS using LC-MS/MS provides many advantages, including the ability to reliably detect new drugs and their metabolites from a variety of biological matrices.
Using Scheduled Ionization to reduce system ion load for proteomics data acquisition by Alexandra Antonoplis | Data acquisition, Ion sources, Life Science Research, Proteomics, QTOF, QTRAP / Triple Quad, SCIEX information, SCIEX OS software, ZenoTOF 7600 systemWhen analyzing highly complex samples from biological matrices, there can be significant amounts of material that elute in the wash cycle of the LC run, depending on the up-front sample preparation used. The Scheduled Ionization mode, available in both SCIEX OS...
Assess the performance of the Echo® MS system by Christie Hunter | Echo® MS+ system, Optimizing Performance, SCIEX information, TechnologyTo obtain the best, most reproducible results using the Echo MS system, it is important to select the best solvent for your analyte and matrix and to ensure the flow rate is optimized for your solvent. Please review this flow rate optimization community post to...
Excel macro for plate building for transformation of 96-well to 384-well plates with generation of batch lists for SCIEX OS software by Mackenzie Pearson | Bioanalysis/PK, Data acquisition, Echo® MS+ system, Pharma, Research (RUO), SCIEX informationThis macro-enabled workbook is designed to help with creating a formatted analysis list for the Echo® MS system, using 96-well plate maps or lists
Back to the new basics: Part 1 | Making the leap from GC-MS to LC-MS by Alex Liu | Biopharma, Blogs, Clinical, Environmental / Industrial, Food / Beverage, Forensic, Life Science Research, PharmaProducing accurate results quickly in a demanding environment is no easy feat for analytical scientists. What’s more, many of us are constantly questioning ourselves—I certainly am—about whether we are employing the best technique for the analysis at hand. It’s an overwhelming thought, considering the wide range of tools that are available to choose from, each of which offers varying levels of capacity, sensitivity, selectivity, specificity and cost. How do you meet the unique needs of your organization without breaking the bank? I get it, and I’m not here to convince you it’s easy. My aim is to guide you through the process to help you make the right decision for you.
How to optimize sample plating to run Echo MS system in ‘Fast acquisition mode’ by Mackenzie Pearson | Data acquisition, Echo® MS+ system, Optimizing Performance, Pharma, SCIEX information, TechnologyThe Echo MS system can acquire data extremely rapidly, at 1 second per sample. To achieve this speed, it should be ensured that there is adequate time between ejections for the analyte signal to return to baseline between ejections. Plating your samples such that you...
Standard addition workflow – for quantification and calculating background levels by April Quinn-Paquet | Bioanalysis/PK, Data processing, Pharma, QTOF, QTRAP / Triple Quad, SCIEX information, SCIEX OS software, Technology, ZenoTOF 7600 systemThe method of standard addition is a quantitative analysis approach used in situations where matrix effects from complex samples contributes to the analytical signal. This makes it impossible to compare the analytical signal between sample and standard using a...
Decision Rules in SCIEX OS software by April Quinn-Paquet | Data acquisition, How-to videos, SCIEX OS software, TechnologyLearn how Decision Rules in SCIEX OS software can be used during acquisition to take corrective actions on the sample queue. This will help you save time and reduce sample losses during acquisition. RUO-MKT-11-13239-A
How do I define the experimental design (the metadata) for my SWATH acquisition study within the OneOmics suite? What are the requirement for replicates? by Christie Hunter | Data processing, Life Science Research, Metabolomics, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemIn quantitative Omics research, the goal is to understand which analytes (protein or metabolite) are perturbed between experimental conditions; therefore we carefully design our studies to explore these questions. The algorithms used within the Assembler application...
What is the Most likely ratio normalization strategy? by Christie Hunter | Algorithms, Life Science Research, MarkerView, Metabolomics, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemWhen performing LC-MS quantitation, there are numerous sources of experimental variance that can confound the quality of your results (variation in the starting amount of sample, variation in the LC-MS measurements, etc.). Having a robust normalization strategy that...
What are my normalization options in MarkerView software and when should I use them? by Christie Hunter | Algorithms, Data processing, MarkerView, SCIEX information, TechnologyIn an LC-MS experiment there are multiple sources of variance that can confound the quality of your results. This variation can be biological e.g. differences between treated and control groups, but can also be non-biological, usually from small variations in...
Top questions about the exposome of PFAS revealed by Simon Roberts | Blogs, Clinical, Environmental / Industrial, Life Science ResearchAccording to the CDC, the exposome is “the measure of all the exposures of an individual in a lifetime and how those exposures relate to health.”
Breaking down the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready by Jianru Stahl-Zeng | Blogs, ClinicalSensitivity and robustness carry different meanings in the world of mass spectrometry. Generally, sensitivity refers to an instrument’s ability to achieve lower limits of detection (LOD). Robustness, on the other hand, refers to an instrument’s ability to consistently...
A rising star in food allergen research: proteomics of shellfish allergen by SCIEX Community | Blogs, Food / Beverage, Life Science Research, ProteomicsIt’s important to know what you’re eating, especially if you suffer from a food allergy. About 220 million people worldwide live with a food allergy.1 These numbers, along with the complexity and severity of conditions, continue to rise. In America, there are about 32 million food allergy sufferers—5.6 million of those are children under the age of 18.2.2 That’s 1 out of every 13 children, or about 2 in every classroom. From a financial perspective, the cost of food allergy childcare for US families is up to $25 billion