Monitoring product quality attributes (PQAs) throughout monoclonal antibody (mAb) development is vital to ensuring drug safety and efficacy. By adopting orthogonal analytical techniques and integrating new technologies that have the potential to provide more information, it is possible to improve product quality and manufacturing efficiency and make more informed decisions.
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Guide decisions during cell line development with more information at the intact level
Monitoring product quality attributes (PQAs) throughout monoclonal antibody (mAb) development is vital to ensuring drug safety and efficacy. By adopting orthogonal analytical techniques and integrating new technologies that have the potential to provide more information, it is possible to improve product quality and manufacturing efficiency and make more informed decisions.
Relative migration time
How do I set up my processing method to calculate the RRT of the main peak to 10kD in karat 32 software?
Current proteomics software compatibility for ZenoTOF 7600 system
Below is a summary of various other software packages that are useful for processing proteomics data from the ZenoTOF 7600 system. Note this list is not comprehensive and only covers the tools we have lightly tested to date. Acquisition Type Software Files needed...
Excel macro for plate building for transformation of 96-well to 384-well plates with generation of batch lists for SCIEX OS software
This macro-enabled workbook is designed to help with creating a formatted analysis list for the Echo® MS system, using 96-well plate maps or lists
sMRM Pro Builder template tutorial
The sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.
MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system
General recommendations when beginning method development Objective: The purpose of this document is to provide a quick reference for transferring MRM-based quantification methods from a SCIEX Triple Quad or QTRAP 6500+ system to a SCIEX 7500 system. While the best...
What is the difference between MRM3 vs MS/MS/MS (MS3)?
The MRM3 workflow and the MS3 scan are functionally the same QTRAP system scan, but used with different goals in mind. The main difference is how these scans are used in the whole MS workflow. With MS3 scans, you can use these in a data dependent mode for discovery...
How do I define the experimental design (the metadata) for my SWATH acquisition study within the OneOmics suite? What are the requirement for replicates?
In quantitative Omics research, the goal is to understand which analytes (protein or metabolite) are perturbed between experimental conditions; therefore we carefully design our studies to explore these questions. The algorithms used within the Assembler application...
Quickly compare identification results from ProteinPilot software
When a ProteinPilot Software search is complete, a ProteinPilot report is generated that contains all the false discovery rate (FDR) analysis information. More information on using the large and small ProteinPilot reports can be found here When doing method...
Breaking down the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready
Sensitivity and robustness carry different meanings in the world of mass spectrometry. Generally, sensitivity refers to an instrument’s ability to achieve lower limits of detection (LOD). Robustness, on the other hand, refers to an instrument’s ability to consistently...
The top 5 questions to ask when investing in accurate mass technology for forensic toxicology workflows
Are you considering the purchase of a high-resolution accurate mass (HRAM) instrument for your forensic toxicology lab? To help ensure you invest in a solution that ideally meets your needs, ask yourself the following key questions. 1. How do I ensure my results...
A rising star in food allergen research: proteomics of shellfish allergen
It’s important to know what you’re eating, especially if you suffer from a food allergy.
About 220 million people worldwide live with a food allergy.1 These numbers, along with the complexity and severity of conditions, continue to rise. In America, there are about 32 million food allergy sufferers—5.6 million of those are children under the age of 18.2.2 That’s 1 out of every 13 children, or about 2 in every classroom. From a financial perspective, the cost of food allergy childcare for US families is up to $25 billion
Calling SCIEX Software Users: Windows 10 Support for all SCIEX Software
As a researcher in a busy lab, the software driving your work is critical to your success, and the timely transition of SCIEX applications to Windows 10 is no exception. In early 2020 Microsoft will be ending Windows 7 support, and we want you to know we are taking...
Detect the Signal, Not the Noise
Improving the specificity and selectivity of your assay Your LC-MS assay is only as good as its power to discern your target compound from everything else. Standards dissolved in clean solvents can make beautiful assays, but analytes in real-world samples can behave...
Software Licensing Comparison: Subscription or Perpetual?
Are you confused by software licensing? Do you want to know the difference between a subscription and a perpetual license? In this blog, we compare both options and explain the value of each choice for your laboratory. Years ago, the perpetual license model was the...
Make the Leap from GC to LC-MS/MS
Choosing the best technique for your analysis can be tough. Should you go with gas chromatography/mass spectrometry (GC-MS) or liquid chromatography/tandem mass spectrometry (LC-MS/MS)? That’s the key question. That’s why we’re here to help. The Limitations of...
Sensitivity, It’s at the Very Heart of Who We Are
Walk into any modern pharmaceutical company these days, and you’ll likely find at least one if not many, SCIEX LC-MS/MS instruments. Assays for the detection and quantitation of small molecule drugs, metabolites, biotherapeutics, biomarkers, and many other analytes...
Uncovering the Links Between Childhood Growth, Body Size, and a Woman’s Risk of Breast Cancer
Welcome to the second in a series of posts marking International Women’s Day, and our ongoing support of World Cancer Research Fund. This installment is a review by Dr. Jennifer Baker, of her work, that, with the help of a WCRF grant, is studying body size and its links to breast cancer. Dr. Baker, Lead Investigator at Denmark’s Frederiksberg Hospital, has a Ph.D. in Human Nutrition from Cornell University. Her research focuses on clinical epidemiology.
A Multi-Omics Database for Tomato Research and Breeding
Ace. Beefsteak. Big Boy. Kumato. Early Girl. Roma. Sun Gold. San Marzano. These are just a few of the thousands of varieties of tomato plants available today. And while all of these varieties may be very different with respect to crop yield, disease resistance,...
Pathway to Success: Young Metabolomics Researchers Named in The Analytical Scientist’s Power List
The direct correlation of the metabolome to the phenotype means metabolomics is one of the most sought-after approaches for the study of disease and wellness, yet the separation, detection, quantification, and unambiguous identification of a chemically diverse...
Metabolomics Studies Benefit Biomedical Research
Professor Dr. Thomas Hankemeier, Head of the Division of Systems Biomedicine and Pharmacology, LACDRLACDR is a center of excellence for multidisciplinary research into drug discovery and development, with a strong focus on metabolomics. As part of its research...
Maximize the use of your software. Buy only what you need
The latest releases of Analyst Software 1.7 and SCIEX OS Software 1.4 introduce a new licensing model called concurrent licensing. If you want flexibility and cost savings when purchasing and using your processing software, concurrent licensing is for you. How does...
3 Reasons to Upgrade to Analyst Software 1.7
Would you be surprised to know that the SCIEX QTRAP® and Triple Quad™ mass spectrometry systems are ideally suited to meet the needs of any lab? Even more so as new orders will ship with our flagship Analyst® Software 1.7. The software is the single LC-MS/MS software,...
On Demand Videos from the 2017 Global CESI-MS Symposium
The 2017 Global CESI-MS Symposium brought together KOLs and industry innovators from around the world to share their latest advancements using capillary electrophoresis integrated with electrospray ionization (CESI-MS) within the same device.
Why Microflow HILIC Chromatography for Targeted Metabolomics Applications?
I recently had the opportunity to catch up with Baljit Ubhi to discuss the top questions you’re asking in regards to using Microflow HILIIC Chromatography for Targeted Metabolomics. Here’s what Bal said:
Discover The Benefits of Knowledge Base Articles
Did you know you can access Knowledge Base Articles for trending user questions compiled and answered by SCIEX support experts? Doing so may help to reduce your support calls, not to mention downtime. Instead of waiting for a problem to occur, you can stay on top of it, and be a part of the solution. To give you an idea of trending articles, consider the how this past month saw questions and answers including:
How Easy Is It to Relocate a Mass Spectrometer?
When you’re in the process of moving your lab, across the corridor or to another country, there’s a lot to think about. Adding to the stress, there’s not always a lot of time to plan, or budget allocated for the process, especially in the case of unexpected urgent maintenance work.
SWATH Acquisition – Master of All Trades
SWATH® Acquisition is an innovative strategy for acquiring data on a TripleTOF® mass spectrometer. In a previous blog, we learned how SWATH works. Now let’s learn what it can do for different applications:
Data Independent Acquisition Mass Spectrometry with the Power of SWATH
There are many different methods in use today to acquire data on a mass spectrometer, but few have generated as much buzz in recent years as SWATH technology. First reported 5 years ago by Ruedi Aebersold and his group1, SWATH® Acquisition on a TripleTOF® instrument has rapidly become one of the premier acquisition strategies for identification and quantitation of complex samples. But what exactly is SWATH and why is it so powerful? In order to answer these questions, let’s first take a step back and look at the larger picture.
Vice President Biden Announces Agreement Naming Children’s Medical Research Institute’s ProCan Lab to the ‘Cancer Moonshot’ Initiative
A key goal of the ‘Cancer Moonshot’ initiative is the advancement of precision medicine, with the goal of making more targeted therapies available to more cancer patients. And researchers believe that the time is right, with the new technological innovations, the new insight into the biology of cancer and big improvements in the handling of ‘big data.’