Unlock the benefits of nominal mass spectrometry for NPS analysis by Pierre Negri | 0 CommentsThe development of analytical methods for the detection and quantitation of drugs and metabolites in a range of biological matrices is a challenging process. Forensic toxicology labs need a reproducible and reliable methodology to ensure the robustness of the data and the quality of the results. They also need robust and sensitive instrumentation that can detect drugs at trace levels with high specificity, especially when it comes to novel psychoactive substances (NPS), which can be difficult to monitor and control.
Jon MacGregor by Jon MacGregor | 0 CommentsI have a turbo v ion source that shows a glowing blue/purple light at the tip of the Heated Nebulizer corona discharge needle, even when it is not in use and turned out of ion path as it should be when using TIS probe. It only happens in negative mode. It is not arcing to the cutain plate, just a slight noticeable glow at the tip of the corona needle when observed through the front window. My FSE has rebuilt the HN probe and components and also installed a new TIS probe. Still glows when in negative mode. Note also having poor sensitivity in negative mode as well and jagged peak shapes during infusion. Any ideas? Are the symptoms related?
Easy switching of sources and LC flow regimes on the ZenoTOF 7600 system by Christie Hunter | 7 CommentsThis series of videos outlines how a user can easily switch sources and operate the system in different LC flow regimes.
Using Scheduled Ionization to reduce system ion load for proteomics data acquisition by Alexandra Antonoplis | 0 CommentsWhen analyzing highly complex samples from biological matrices, there can be significant amounts of material that elute in the wash cycle of the LC run, depending on the up-front sample preparation used. The Scheduled Ionization mode, available in both SCIEX OS...
Unlock the benefits of nominal mass spectrometry for NPS analysis by Pierre Negri | Blogs, Clinical, Forensic, QTRAP / Triple Quad, ToxicologyThe development of analytical methods for the detection and quantitation of drugs and metabolites in a range of biological matrices is a challenging process. Forensic toxicology labs need a reproducible and reliable methodology to ensure the robustness of the data and the quality of the results. They also need robust and sensitive instrumentation that can detect drugs at trace levels with high specificity, especially when it comes to novel psychoactive substances (NPS), which can be difficult to monitor and control.
Easy switching of sources and LC flow regimes on the ZenoTOF 7600 system by Christie Hunter | Data acquisition, Ion sources, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemThis series of videos outlines how a user can easily switch sources and operate the system in different LC flow regimes.
Using Scheduled Ionization to reduce system ion load for proteomics data acquisition by Alexandra Antonoplis | Data acquisition, Ion sources, Life Science Research, Proteomics, QTOF, QTRAP / Triple Quad, SCIEX information, SCIEX OS software, ZenoTOF 7600 systemWhen analyzing highly complex samples from biological matrices, there can be significant amounts of material that elute in the wash cycle of the LC run, depending on the up-front sample preparation used. The Scheduled Ionization mode, available in both SCIEX OS...
General source/gas settings to use when starting method development by Christie Hunter | Data acquisition, QTRAP / Triple Quad, SCIEX information, TechnologyHere, we provide a quick reference for the source/gas settings to use at various flow rates with Turbo V, IonDrive Turbo V and OptiFlow Pro ion sources that come with the various SCIEX mass spectrometers. While performing a full source/gas optimization can provide the...
MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system by Christie Hunter | Bioanalysis/PK, Data processing, Methods / workflows, Pharma, QTRAP / Triple Quad, SCIEX information, SCIEX OS softwareGeneral recommendations when beginning method development Objective: The purpose of this document is to provide a quick reference for transferring MRM-based quantification methods from a SCIEX Triple Quad or QTRAP 6500+ system to a SCIEX 7500 system. While the best...
What is SWATH acquisition and what are the critical acquisition attributes? by Christie Hunter | Data acquisition, Food / Beverage, Food and Beverage, Forensic, Fundamentals, Life Science Research, Metabolomics, OneOmics, Pharma, Proteomics, QTOF, SCIEX information, Toxicology, ZenoTOF 7600 systemIn data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition,...
How do I add a retention time calibration protein to my SWATH acquisition ion library? by Christie Hunter | Data processing, Life Science Research, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemWhen processing SWATH acquisition data, you want to use a reasonably narrow time window to reduce the chance of incorrect peak integrations and to reduce data processing time. There are currently two strategies being employed today to adjust for differences between...
Breaking down the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready by Jianru Stahl-Zeng | Blogs, ClinicalSensitivity and robustness carry different meanings in the world of mass spectrometry. Generally, sensitivity refers to an instrument’s ability to achieve lower limits of detection (LOD). Robustness, on the other hand, refers to an instrument’s ability to consistently...
The top 5 questions to ask when investing in accurate mass technology for forensic toxicology workflows by Holly McCall | Blogs, ForensicAre you considering the purchase of a high-resolution accurate mass (HRAM) instrument for your forensic toxicology lab? To help ensure you invest in a solution that ideally meets your needs, ask yourself the following key questions. 1. How do I ensure my results...
A rising star in food allergen research: proteomics of shellfish allergen by SCIEX Community | Blogs, Food / Beverage, Life Science Research, ProteomicsIt’s important to know what you’re eating, especially if you suffer from a food allergy. About 220 million people worldwide live with a food allergy.1 These numbers, along with the complexity and severity of conditions, continue to rise. In America, there are about 32 million food allergy sufferers—5.6 million of those are children under the age of 18.2.2 That’s 1 out of every 13 children, or about 2 in every classroom. From a financial perspective, the cost of food allergy childcare for US families is up to $25 billion
Calling SCIEX Software Users: Windows 10 Support for all SCIEX Software by SCIEX Community | Blogs, Software, TechnologyAs a researcher in a busy lab, the software driving your work is critical to your success, and the timely transition of SCIEX applications to Windows 10 is no exception. In early 2020 Microsoft will be ending Windows 7 support, and we want you to know we are taking...
Detect the Signal, Not the Noise by SCIEX Community | Blogs, Technology Improving the specificity and selectivity of your assay Your LC-MS assay is only as good as its power to discern your target compound from everything else. Standards dissolved in clean solvents can make beautiful assays, but analytes in real-world samples can behave...
Software Licensing Comparison: Subscription or Perpetual? by SCIEX Community | Blogs, Software, Technology Are you confused by software licensing? Do you want to know the difference between a subscription and a perpetual license? In this blog, we compare both options and explain the value of each choice for your laboratory. Years ago, the perpetual license model was the...
Make the Leap from GC to LC-MS/MS by SCIEX Community | Blogs, Technology Choosing the best technique for your analysis can be tough. Should you go with gas chromatography/mass spectrometry (GC-MS) or liquid chromatography/tandem mass spectrometry (LC-MS/MS)? That’s the key question. That’s why we’re here to help. The Limitations of...
Sensitivity, It’s at the Very Heart of Who We Are by Christie Hunter | Blogs, Technology Walk into any modern pharmaceutical company these days, and you’ll likely find at least one if not many, SCIEX LC-MS/MS instruments. Assays for the detection and quantitation of small molecule drugs, metabolites, biotherapeutics, biomarkers, and many other analytes...
Uncovering the Links Between Childhood Growth, Body Size, and a Woman’s Risk of Breast Cancer by SCIEX Community | Blogs, Life Science ResearchWelcome to the second in a series of posts marking International Women’s Day, and our ongoing support of World Cancer Research Fund. This installment is a review by Dr. Jennifer Baker, of her work, that, with the help of a WCRF grant, is studying body size and its links to breast cancer. Dr. Baker, Lead Investigator at Denmark’s Frederiksberg Hospital, has a Ph.D. in Human Nutrition from Cornell University. Her research focuses on clinical epidemiology.
A Multi-Omics Database for Tomato Research and Breeding by SCIEX Community | Blogs, Life Science Research, Metabolomics Ace. Beefsteak. Big Boy. Kumato. Early Girl. Roma. Sun Gold. San Marzano. These are just a few of the thousands of varieties of tomato plants available today. And while all of these varieties may be very different with respect to crop yield, disease resistance,...
Pathway to Success: Young Metabolomics Researchers Named in The Analytical Scientist’s Power List by SCIEX Community | Blogs, Life Science Research, Metabolomics The direct correlation of the metabolome to the phenotype means metabolomics is one of the most sought-after approaches for the study of disease and wellness, yet the separation, detection, quantification, and unambiguous identification of a chemically diverse...
Metabolomics Studies Benefit Biomedical Research by SCIEX Community | Blogs, Life Science Research, MetabolomicsProfessor Dr. Thomas Hankemeier, Head of the Division of Systems Biomedicine and Pharmacology, LACDRLACDR is a center of excellence for multidisciplinary research into drug discovery and development, with a strong focus on metabolomics. As part of its research...
Maximize the use of your software. Buy only what you need by SCIEX Community | Blogs, Software, Technology The latest releases of Analyst Software 1.7 and SCIEX OS Software 1.4 introduce a new licensing model called concurrent licensing. If you want flexibility and cost savings when purchasing and using your processing software, concurrent licensing is for you. How does...
3 Reasons to Upgrade to Analyst Software 1.7 by SCIEX Community | Blogs, Software, TechnologyWould you be surprised to know that the SCIEX QTRAP® and Triple Quad™ mass spectrometry systems are ideally suited to meet the needs of any lab? Even more so as new orders will ship with our flagship Analyst® Software 1.7. The software is the single LC-MS/MS software,...
On Demand Videos from the 2017 Global CESI-MS Symposium by Kelly May | Biopharma, Blogs, Life Science ResearchThe 2017 Global CESI-MS Symposium brought together KOLs and industry innovators from around the world to share their latest advancements using capillary electrophoresis integrated with electrospray ionization (CESI-MS) within the same device.
Why Microflow HILIC Chromatography for Targeted Metabolomics Applications? by SCIEX Community | Blogs, Life Science Research, MetabolomicsI recently had the opportunity to catch up with Baljit Ubhi to discuss the top questions you’re asking in regards to using Microflow HILIIC Chromatography for Targeted Metabolomics. Here’s what Bal said:
Discover The Benefits of Knowledge Base Articles by SCIEX Community | Blogs, TechnologyDid you know you can access Knowledge Base Articles for trending user questions compiled and answered by SCIEX support experts? Doing so may help to reduce your support calls, not to mention downtime. Instead of waiting for a problem to occur, you can stay on top of it, and be a part of the solution. To give you an idea of trending articles, consider the how this past month saw questions and answers including:
How Easy Is It to Relocate a Mass Spectrometer? by SCIEX Community | Blogs, TechnologyWhen you’re in the process of moving your lab, across the corridor or to another country, there’s a lot to think about. Adding to the stress, there’s not always a lot of time to plan, or budget allocated for the process, especially in the case of unexpected urgent maintenance work.
SWATH Acquisition – Master of All Trades by SCIEX Community | Blogs, TechnologySWATH® Acquisition is an innovative strategy for acquiring data on a TripleTOF® mass spectrometer. In a previous blog, we learned how SWATH works. Now let’s learn what it can do for different applications:
Data Independent Acquisition Mass Spectrometry with the Power of SWATH by SCIEX Community | Blogs, Life Science Research, Metabolomics, ProteomicsThere are many different methods in use today to acquire data on a mass spectrometer, but few have generated as much buzz in recent years as SWATH technology. First reported 5 years ago by Ruedi Aebersold and his group1, SWATH® Acquisition on a TripleTOF® instrument has rapidly become one of the premier acquisition strategies for identification and quantitation of complex samples. But what exactly is SWATH and why is it so powerful? In order to answer these questions, let’s first take a step back and look at the larger picture.
Vice President Biden Announces Agreement Naming Children’s Medical Research Institute’s ProCan Lab to the ‘Cancer Moonshot’ Initiative by SCIEX Community | Blogs, Life Science Research, ProteomicsA key goal of the ‘Cancer Moonshot’ initiative is the advancement of precision medicine, with the goal of making more targeted therapies available to more cancer patients. And researchers believe that the time is right, with the new technological innovations, the new insight into the biology of cancer and big improvements in the handling of ‘big data.’
Stoller Biomarker Discovery Centre, Addressing Some of the Biggest Issues in Medicine by SCIEX Community | Blogs, Life Science Research, ProteomicsThe Stoller Biomarker Discovery Center, developed in partnership with SCIEX, was created to develop new omics technologies for biomarker research to understand the root cause of diseases such as cancer, cardiovascular disease, and autoimmune diseases. We initially announced our collaboration with the University of Manchester back in October 2015.
The History of Isotopic Labels for Quantitative Proteomics by Subhasish.Purkayastha@sciex.com | Blogs, Life Science Research, ProteomicsProteomics has become a vital tool for biological scientists performing research on the healthy and diseased states of living things. It involves the large scale and systematic analysis of all proteins within a given cell, tissue, or organism. Because proteins are regulated by many different internal and external stimuli, the proteome is dynamic and quantities of proteins can change from one state to the next. Therefore, in order to be of the highest utility, proteomics experiments need to both identify and quantify proteins so that comparative studies can be done, such as between healthy cells and tumor cells, or the comparison of different treatment regimens.