Do you want a more efficient workflow for the forensic analysis of THC-COOH in hair samples? Yes! Do we know a simple, highly sensitive technique? You bet! Read on to find out how you can detect THC-COOH in hair down to 0.2 pg/mg trace concentration levels with...
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Celebrating customer experience: Insights from SCIEX leaders
Introduction Customer Experience Day (CX Day) is a special occasion for SCIEX, celebrated every first Tuesday in October. It’s a day dedicated to recognizing the incredible value of our customers and the relentless dedication of our associates who strive to make...
LC-MS system replacement: Are you ready?
Meeting deadlines in a bioanalysis laboratory can be a big challenge. Older, less sensitive and less reliable LC-MS systems make it even more difficult. Even the disruption caused by the installation and validation can be disconcerting and delay decisions. Does this sound familiar?
Questions and answers to help improve your mycotoxin analysis
During a recent webinar I shared method details for mycotoxin analysis on the SCIEX 7500 system. In this blog i will share the Q&A for the submitted questions that we did not have chance to answer during the live webinar.
Optimized rolling collision energy curves for IDA and SWATH DIA for peptides
During data dependent acquisition (DDA or IDA) or SWATH acquisition, the collision energy can be automatically adjusted according to the mass/charge and charge of the peptide. This dependency has been well characterized on our QTOF systems. By selecting rolling...
Back to the new basics: Part 1 | Making the leap from GC-MS to LC-MS
Producing accurate results quickly in a demanding environment is no easy feat for analytical scientists. What’s more, many of us are constantly questioning ourselves—I certainly am—about whether we are employing the best technique for the analysis at hand.
It’s an overwhelming thought, considering the wide range of tools that are available to choose from, each of which offers varying levels of capacity, sensitivity, selectivity, specificity and cost. How do you meet the unique needs of your organization without breaking the bank? I get it, and I’m not here to convince you it’s easy. My aim is to guide you through the process to help you make the right decision for you.
MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system
General recommendations when beginning method development Objective: The purpose of this document is to provide a quick reference for transferring MRM-based quantification methods from a SCIEX Triple Quad or QTRAP 6500+ system to a SCIEX 7500 system. While the best...
Adapting a SCIEX high flow source for microflow LC
To set up a SCIEX high flow source for microflow LC (Turbo V ion source, DuoSpray source or IonDrive Turbo V ion source), first you must replace the wider bore electrodes with more narrow bore hybrid electrodes. Note with the OptiFlow Turbo V ion sources, there are...
Identifying the unknown PFAS profile in firefighting foams/AFFF
According to a recent study from Harvard University, the US EPA, and NIEHS, traditional targeted analysis techniques poorly characterize the PFAS composition of contemporary PFAS-based firefighting foams, know as aqueous film-forming foams (AFFF). Using the EPA 533 PFAS drinking water method for the analyte list, the researchers found that targeted mass spectrometry methods accounted for <1% of organic fluorine content. This is important because it demonstrates that targeted analysis methods miss nearly all the PFAS compounds in modern AFFF mixtures, thus underestimating the risk to human health and the environment.
sMRM Concurrency Calculator
This excel sheet allows you to quickly estimate the MRM concurrency and approximate dwell time for your Scheduled MRM Algorithm acquisition method. Paste your MRM method with retention times into the input tab then calculate the Excel workbook. Plots will update to...
The risky business of aflatoxins in milk
If you’re in the dairy or food testing business, you know the threat aflatoxins pose. Aflatoxins are a type of mycotoxin produced by Aspergillus parasiticus, aspergillus flavus , and rarely aspergillus nomius.1 These are likely the most extensively researched group of mycotoxins because of their adverse health effects.2 What’s more, they are widely found in a variety of crops, namely maize, tree nuts, and spices. Believed to be primarily caused by rising temperatures and humidity, these naturally occurring fungi grow on crops in the field, or during storage of feed and raw materials, where they can potentially produce toxins that enter the food chain.
The detection of acid herbicides and urons by large volume injection
Pre-treatment versus direct injection – that is the question posed in the application note, “The Detection of Acidic Herbicides and Phenyl Ureas by LC-MS/MS with Large Volume Injection and Automated Column Switching.” It’s just one of the dozens of articles you will find within the Environmental Compendium (pages 1 to 4, pesticides) now available for download.
See What More You Can do With 360 Degree Biologics Characterization
Ever wish you had your own team of mass spectrometry experts at your side when working through biologics development and characterization challenges? With SCIEX 360° Innovations complex biologics characterization is streamlined with a full suite of mass spectrometry (MS), capillary electrophoresis (CE) systems, software, and services from SCIEX experts.
You’ve Seen It… Now Try It! BioPharmaView Software 2.0
At ASMS this year, the newest version of BioPharmaView Software was released. This software simplifies the processing of biotherapeutic data for characterization and comparability which can dramatically improve your productivity. BioPharmaView 2.0 Software accelerates characterization and comparability studies and simplifies reporting, so you can make better decisions, faster.
Quantitation of Antibiotics and Insecticides in Poultry Feed using LC-MS/MS
Quantitating antibiotics and insecticides in poultry is serious business. Overuse can lead to antibiotic resistance while insecticide residuals can cause harmful side effects in humans. In the United States, for example, the Federal Drug Administration (FDA), has offered up a plan to limit common antibiotics in feed, which are used to encourage growth. However, this is a voluntary plan, and as the following application note, “Quantitation of Antibiotics and Insecticides in Poultry Feed using LC-MS/MS,” points out, antibiotics have been shown to accumulate in poultry feathers, which are in turn used for nutritional elements in the feed.
Screening Novel Psychoactive Substances with Confidence
How do you know what you can’t see? This is the challenge many a lab faces as they relentlessly test for novel psychoactive substances (NPS) as unknown samples with an ever-changing ingredient list make discovery difficult work at best. There are many reasons for the complexities of which you can discover in this application note, “Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances.” However, the biggest of which is that non-targeted findings can turn up thousands of molecular features in a single sample. Sifting through the peaks is laborious, and many are normal besides.
Perfluoroalkyl Acids in Drinking Water – EPA Method 537
The United States Environmental Protection Agency (EPA), under the 1996 Safe Drinking Water Act (SDWA), requires a new list of no more than 30 unregulated contaminants to be monitored by public drinking water systems. Known as the Unregulated Contaminant Monitoring Rule (UCMR), a new list is published every five years. The last rule, UCMR3, was published May 2, 2012, and is the focus of the following application note, “Analysis of Perfluoroalkyl (PFFA) Acids Specified under the UCMR3 Using the QTRAP® 6500 LC-MS/MS system,” which can be found in the Food and Environmental Compendium.
SCIEX helps set food standards in China
One of the biggest concerns of Chinese citizens is food safety1. Even though China ranks second in global economies2, crowding, industrial pollution, labor and certain agriculture practices have contributed to this. In October 2015, however, we began to see a turnaround as the Chinese government revised its 2009 Food Safety Law in an attempt to strengthen its food supply oversight and quality.
Discover the new and accurate SCIEX way to enhance your routine food allergen testing
Food allergy is an immune-mediated, adverse reaction to an antigenic protein. Even limited exposure to an antigen can provoke a significant reaction in sensitive individuals, causing rashes, itching and swelling in the mouth, nausea, vomiting, and asthma. Additionally, food allergies are the leading cause of anaphylaxis, an acute, potentially deadly allergic reaction. The prevalence and severity of food allergies are rising, with approximately 150 million people suffering from food allergies worldwide.1, 2 Presently, there is no cure for food allergies, and sufferers must rely on the correct labeling of foods to avoid consuming allergens. Hence, the development of sensitive and accurate analytical methods to screen for the presence of allergens in food products is necessary for the prevention of potentially life-threatening health problems for allergy sufferers.
Three Infographics to Show You How to Overcome Challenges in Transitioning to Biologics Bioanalysis
The move to large molecules in Pharma is accelerating, offering unprecedented opportunities to improve human health and expand into new markets. But for those with extensive experience with small molecule bioanalysis, the shift to biologics can be challenging, from Sample Prep to Instrumentation and Software, to Methods and Training:
Rapid Characterization of Biologics using CESI-MS
Today, 30 monoclonal antibodies (mAbs), have been approved for the treatment of certain cancers, autoimmune and infectious diseases. Even more are in development, and perhaps you and your team of scientists are working on one now. Keeping pace with fast development timelines while performing comprehensive characterization of biologic candidates can be challenging. However, more and more, scientists are tackling these challenges with new techniques to speed and simplify their characterization workflows. Read more in the application note, “Rapid Characterization of Biologics using a CESI 8000 – SCIEX TripleTOF® System,” found in the Biologics Analytical Characterization Compendium, which highlights how CESI separation coupled with high-resolution mass spectrometry can provide a comprehensive characterization of biotherapeutics.
The Not So Hidden Truth about Climate Change How It’s Poisoning Your Food
Did you know climate change could be poisoning your food? According to the United Nations Environmental Program (UNEP) report on Emerging Issues of Environmental Concern, rising temperatures are making crops more toxic.
Guardians of Antibiotics
This second is a blog series on the global war: Rise of Superbugs! Part 1 took a critical look at the antibiotic threat we face in today’s battlefield. The waning effectiveness of antibiotics as we head into what may seem like a post-antibiotic era has impelled new reformation to at the very least control antibiotic usage to ensure food safety.
Protein Quantitation Workflows using the TripleTOF 6600: A Case Study for Rituximab
Although the triple-stage quadrupole (QQQ) mass spectrometer remains the pillar for quantitative LC-MS/MS bioanalytical assays, due in part to the platforms’ high duty cycle when operated in multiple-reaction monitoring (MRM) mode, the applicability of high-resolution mass spectrometry (HRMS) has become of increasing importance for protein quantitation given the complexity of proteolytically digested samples in the surrogate peptide approach. While the QQQ demonstrates high sensitivity and specificity, the relatively low-resolution measurement of m/z may fail to differentiate analyte response from nominally isobaric background interference. In contrast, HRMS with accurate mass assignment of product ion allows interference to be resolved through judicious selection of a post-acquisition mass extraction window whose tolerance is largely dictated by the effective resolution and stability of mass calibration.
Rise of the Super Bugs
The term “antibiotic-free” is becoming more and more popular in food advertising these days. Take Subway for example; in March the company elevated their antibiotic-free policy and introduced a new antibiotic-free rotisserie-style chicken sub, and they plan to, “Nix antibiotics in all its meat by 2025.”
Glyphosate, a Polar Pest Put to Test
No other pesticide has courted more media attention and controversy in recent months than glyphosate, with governments and national agencies debating its use and health effects.
Using Mass Spec to Detect Trace Explosives
The importance of protecting a country’s border is a very topical issue. The war on drugs and terror is a 24/7 task 366 days a year (2016 is a leap year). The government agencies in charge must be vigilant and maintain instrumentation to prevent terrorism, drug trafficking, and other illegal activities. Mass Spectrometry is rapidly becoming the instrument of choice for border agencies throughout the world when it comes to explosive trace detection and forensic drug compounds.
Routine Food Testing Using Mass Spectrometry
These days, it is not uncommon to hear about the overzealous application of pesticides to crops or the injection of antibiotics into animals. From grocery stores to restaurants, our food is at risk. How then, can consumers be assured that chemical contaminants like these , not to mention the risk of mycotoxin compounds are not making their way to your dinner table?
Quantify and Identify Pesticides in Complex Food Samples Using the QTRAP 6500 LC-MS/MS System
Recent regulations on food analysis require screening for pesticides using confirmatory techniques, such as GC-MS and LC-MS/MS. More than 1000 pesticides are used worldwide and, along with their metabolites and degradation products, are present in food. There is a demand for powerful and rapid analytical methods that can identify pesticides with high confidence in a broad range of food matrices and quantify at low concentrations with good accuracy and reproducibility. Challenges for pesticide residue laboratories at the moment are the request to test for more compounds, in a wider range of samples, all without sacrificing data quality.
Fast, Efficient, Disulfide Bond Mapping Using BioPharmaView™ Software
Fast LC-MS acquisition and automated data processing will help you speed up peptide mapping of your biotherapeutic, including critical disulfide bond and post-translational modification characterization. SCIEX helps you untangle the complexity of disulfide bonds, speeding up your characterization process.
Bottom-Up Proteomics: A Discussion with Christie Hunter
Biocompare recently featured an article on Bottom-Up Proteomics. I had a chance to follow up with Christie Hunter and expand on some of the questions featured in the article: