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sMRM Pro Builder template tutorial
The sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.
- Data processing
- Data Visualization
- How-to videos
- Life Science Research
- Lipidomics
- Metabolomics
- Optimizing Performance
- QTRAP / Triple Quad
- Results
Tags: amount, assay, embed, excel, food, form, help, job, large, money, number, object, pattern, question, retention, sMRM, template, time, use, video, water
November 15, 2021
MRM method transfer from a SCIEX Triple Quad or QTRAP 6500+ system to the SCIEX 7500 system
General recommendations when beginning method development Objective: The purpose of this document is to provide a quick reference for transferring MRM-based quantification methods from a SCIEX Triple Quad or QTRAP 6500+ system to a SCIEX 7500 system. While the best sensitivity can be obtained by performing a full compound/source/gas optimization, the recommendations presented here will […]
- Bioanalysis/PK
- Data processing
- Methods / workflows
- Pharma
- QTRAP / Triple Quad
- SCIEX information
- SCIEX OS software
Tags: change, dissociate, form, high, interference, ion, machine, method, model, money, noise, number, object, period, power, ratio, software, source, system, work
October 29, 2021
Short-chain PFAS compounds are on the rise- Craig’s PFAS Vodcast Cora Young
Read time: 2 minutes Short-chain per- and polyfluoroalkyl substances (PFAS) are increasing in the Canadian Arctic environment, with the most rapid increases occurring post-2000, according to a recent study in Geophysical Research Letters (April 2020). For example, trifluoracetic acid (TFA) in the Devon Ice Cap increased ~10-fold from 1.4 μg/m2 per year during 1977–1989 to […]
Tags: chlorine, compound, disinfection, drug, increase, machine, number, object, personal care, pool, pool water, research, result, science, study, swimming, tap water, time, use, water, water temperature
October 14, 2021
Save time by saving your workspace layout in SCIEX OS software
This video teaches you how to save your workspace layout in Analytics section of SCIEX OS software. Streamline your data visualization by saving optimized workspace layouts for your various data processing workflows. RUO-MKT-18-13321-A
Tags: ambulance, analytic, check, data, embed, layout, picture, project, save, saving, section, software, store, thrift, value, video, visualization, watch, work
September 29, 2021
Adding the PepCalMix to your SWATH acquisition ion Library as a Retention Time Calibration Protein
The most important consideration in adding a new RT calibration protein to your library is making sure you are getting the retention time for both into the same retention time or chromatographic frame. Below is a list of steps that walks you through how to do this. Select a single SWATH acquisition run to act […]
September 28, 2021
How does the scoring and FDR analysis work for SWATH acquisition data processing? For proteomics
Similar extraction processes are used in both the SWATH acquisition microapp in PeakView software on the desktop and the Extractor app in OneOmics suite. After the fragment ions have been selected for each peptide, the extracted ion chromatograms are generated and the sets of XICs generated make up the peptide groups that are scored for […]
How are peptides and transitions selected for use in quantification, when importing from a ProteinPilot software group file – using SWATH acquisition microapp or Extractor in OneOmics suite?
Similar selection processes are used in both the SWATH acquisition microapp in PeakView software on the desktop and the Extractor app in OneOmics suite. During the protein grouping process in ProteinPilot software, there is an extensive analysis that is done on the proteins and peptides that are identified. These results are annotated according to what […]
What software do I need to process my SWATH acquisition data using the desktop or cloud solutions?
Desktop solution: If you are processing your SWATH acquisition data using the SCIEX pipeline, there are a 3 software packages that work together seamlessly for processing. First, you would process your IDA data using ProteinPilot software to create an ion library. Ion libraries only need to be created once, and are loaded into the SWATH […]
What is the best way to build spectral ion libraries for use in processing of proteomics SWATH acquisition data?
The information needed for targeted data extraction in our current SWATH acquisition workflow is relatively simple. You need the parent ion m/z; you need the m/z and relative intensities of the major fragment ions that are produced during MS/MS; and you need to know the relative retention times of the peptides of interest. Currently researchers […]
What is variable window SWATH acquisition and why does it improve results quality?
To continue to improve the quality of the SWATH acquisition data that one can achieve in complex matrices, we are working on ways that we can decrease the size of the Q1 window, while still maintaining full mass range coverage and optimal cycle times. One great way to do this is to use the variable […]
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