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What is SWATH acquisition and what are the critical acquisition attributes?
In data-independent acquisition strategies like SWATH acquisition, an expanded mass isolation window is stepped across a mass range covering the mass-to-charge (m/z) distribution of peptides and a full scan MS/MS spectrum is collected at each step. Post-acquisition, high resolution ion chromatograms are extracted for specific fragment ions of the peptides of interest, and these are […]
- Data acquisition
- Food / Beverage
- Food and Beverage
- Forensic
- Fundamentals
- Life Science Research
- Metabolomics
- OneOmics
- Pharma
- Proteomics
- QTOF
- SCIEX information
- Toxicology
- ZenoTOF 7600 system
Tags: acquisition, attribute, ion, mass, resolution, specificity, spectrum, window
September 28, 2021
How to download and use SVG files from OneOmics suite
Results workspaces in the OneOmics suite contain a variety of figures, all available for download as SVG (scalable vector graphic) files. To download your figures, select the orange arrow icon located in the upper right-hand corner of each plot. Download will immediately begin on your computer and an SVG file will be available for use. […]
Tags: axe, book, change, color, computer, design, document, download, edit, figure, file, image, line, object, picture, select, shape, slide, svg file, way
September 24, 2021
How to share your results with collaborators
As multi-omics research is often highly collaborative, tools for rapidly sharing important results files are needed to drive scientific progress. The SCIEX Data Store in the OneOmics suite enables you to not only organize your uploaded multi-omics data files into studies for further processing, but also to share your studies easily and securely with your […]
Tags: computer, data, file, job, management, organization, result, share, store
Files for running the SWATH acquisition performance kit – ion library, performance trackers
The FASTA file required for use with the SWATH acquisition performance kit installs with ProteinPilot software or is available within OneOmics suite. Download this SWATH acquisition ion library for use with the SWATH acquisition performance kit Human2D_SOP_PepCalMix_ Jan2016 Note: This is a small library that was generated using the K562 human leukemia cell line and […]
- Data processing
- Life Science Research
- Optimizing Performance
- Proteomics
- QTOF
- SCIEX information
- ZenoTOF 7600 system
September 20, 2021
Adapting a SCIEX high flow source for microflow LC
To set up a SCIEX high flow source for microflow LC (Turbo V ion source, DuoSpray source or IonDrive Turbo V ion source), first you must replace the wider bore electrodes with more narrow bore hybrid electrodes. Note with the OptiFlow Turbo V ion sources, there are pre-set probes and electrodes that are already optimized […]
- Data acquisition
- Development
- Ion sources
- LC
- Life Science Research
- Metabolomics
- Pharma
- Proteomics
- SCIEX information
Tags: black, electrode, flow, gas, hybrid, little, machine, money, move, nut, power, pressure, probe, protrusion, run, signal, source, turn, water, work
Troubleshooting low flow LC – LC-MS peak area is lower than expected
Symptom: LC-MS peptide peak are is smaller than expected in PepCalMix Test Test: Use PepCalMix Infusion tests to determine if it is a problem with LC or MS Solution: First perform the SCIEX recommended performance tests using infusion on your MS system. If these standard infusion tests have all passed, then the problem could be […]
Troubleshooting low flow LC – Spread of peptide elution is compressed or delayed
Symptom: The observed retention times of the peptides eluting are compressed, are not as spread out as expected, suggesting the elution gradient is compressed Test: Use PepCalMix LC-MS test to check the peptide elution time and spread Solution: Make sure you are performing the recommended regular maintenance tests on your low flow LC system Ensure […]
Troubleshooting low flow LC – Loss of early eluting peptides during trap-elute workflow
Symptom: Early eluting peptides are missing (based on monitoring PepCalMix LC-MS test or observing a change in the shape of the proteome TIC). Test: Use PepCalMix LC-MS test to monitor binding of early eluting peptides. There are a number of very hydrophilic peptides in the PepCalMix which are great for testing the quality of trapping […]
CloudTalk – Analytics for proteomics
This CloudTalk video (6:34min) describes the use of the Analytics app in OneOmics suite for evaluating the quality of your SWATH acquisition data. RUO-MKT-18-6582-A
CloudTalk – visualize your protein ID results within the ProteinPilot app in OneOmics suite
This CloudTalk video describes how to view ProteinPilot app search results using the Analytics and Browser applications within the ProteinPilot app. To perform a database search on your data using the ProteinPilot app, please watch CloudTalk – data processing with the ProteinPilot app in OneOmics suite RUO-MKT-18-12529-A
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