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What to do if you see peak shape deterioration or jagged peaks in your Echo MS system data?
Once a method has been developed, and good, consistent peak shapes have been obtained for an assay, the Echo® MS system is generally quite consistent. If peak quality deteriorates, consider the following points: The viscosity of the sample can affect the peak shape and cause broadening of the peaks. If your matrix does broaden your […]
- Biopharma
- Data acquisition
- Echo® MS+ system
- Environmental / Industrial
- Food / Beverage
- Life Science Research
- Pharma
- SCIEX information
- Technology
August 24, 2021
Tips to maximize electrode lifetime for Echo MS system
While it’s easy to think of the Echo® MS system as an ultrafast LC system in front of the SCIEX Triple Quad 6500+ mass spectrometer, the system operates on fundamentally different principles. For this reason, it requires different routine maintenance to keep it running at its best. The component of the Echo® MS system most […]
- Biopharma
- Data acquisition
- Echo® MS+ system
- Environmental / Industrial
- Food / Beverage
- Instrument maintenance
- Life Science Research
- Pharma
- SCIEX information
- Technology
Tags: clean, electrode, flow, gas, good, group, high, lifetime, machine, nature, pressure, run, solvent, source, system, temperature, time, usage, water
Echo MS system maintenance operations
The Echo® MS system is a robust platform, and like any other platform, requires some routine maintenance on a regular basis. Here are 3 maintenance steps that can help you ensure consistent, reliable performance: Immediately before use or after switching carrier solvents, purge the carrier solvent at 1,000 µL/min for 5 minutes. This ensures there […]
- Biopharma
- Echo® MS+ system
- Environmental / Industrial
- Food / Beverage
- Instrument maintenance
- Life Science Research
- Pharma
- SCIEX information
- Technology
Tags: acoustic, carrier, change, couple, ensure, error, flow, fluid, gas, line, liquid, nature, period, purge, run, solvent, system, tear, time, water
sMRM Concurrency Calculator
This excel sheet allows you to quickly estimate the MRM concurrency and approximate dwell time for your Scheduled MRM Algorithm acquisition method. Paste your MRM method with retention times into the input tab then calculate the Excel workbook. Plots will update to show you the approximate concurrency across your LC gradient and the approximate dwell times. You can then […]
Tags: amount, approximate, book, border, computing, dwell, estimate, excel, file, impact, method, model, money, number, plot, power, stay, times, visualize
August 20, 2021
Using .csv files to quickly assign metadata for large-scale studies in OneOmics suite
In the Experiments App within the OneOmics suite, metadata can be quickly assigned for large-scale studies using .csv files. To use .csv files for metadata assignment, first create a metabolomics or proteomics SWATH acquisition experiment and select .wiff files for processing. Then go to the metadata Samples Editor and click the download icon to download […]
August 11, 2021
How does FDR scoring work in the OneOmics suite for metabolomics?
To assess the statistical accuracy of library identifications, the Extractor App located within the Metabolomics App in the OneOmics suite features built-in false discovery rate (FDR) scoring for processing metabolomics data. The FDR scoring follows a target-decoy approach, where decoys are generated directly from the library used for data processing. The following criteria are used […]
Using custom ion libraries for metabolomics SWATH acquisition data processing in the OneOmics suite
With the Metabolomics App in the OneOmics suite, it is possible to upload a custom ion library for peak extraction by the Extractor App during the processing of SWATH acquisition data. When creating an ion library for use in the Metabolomics app, be sure to create a separate ion library for use with samples acquired […]
Uploading and using transcriptomics data in the OneOmics suite
RNA experiments can be created in the OneOmics suite for multi-omics analyses, enabling integration of transcriptomics data and proteomics data for biological insight. To build RNA experiments, either CloudConnect for PeakView software 2.2 or BaseSpace (Illumina) can be used to upload transcriptomics data files for use within the SCIEX Cloud Platform. If using BaseSpace, ensure […]
Tags: article, book, build, business, cloud, company, create, data, diff file, experiment, file, folder, gene, machine, object, project, text, text file, way, work
Clustering algorithms in MarkerView App
Finding interesting and significant changes across a biological dataset can be challenging, so having good algorithms that can mine large datasets for differences, and find the statistically significant changes is really important. Within the MarkerView App we have two different options, principal component analysis (PCA) with principal component variable grouping (PCVG) and K-means clustering. PCA-PCVG […]
Tags: analysis, cluster, fold change, group, principal, seed
Merging two FASTA files
There is an easy way to merge two FASTA files together. Open notepad and type this string: type %*>”newfile.fasta” Enter any name for the FASTA file between the quotes. Save the file as: Merge FASTA files.bat Copy the two files you want to merge into the same folder. Drag and drop the two files onto […]
Tags: bat, book, computer, copy, cut, drag, drop, easy, fasta file, file, folder, format, merge, new, right, shape, topic, type, way, word
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