Neonicotinoids have become the most widely used class of insecticide in the world, with registration in 120 countries. However, these pesticides have become embroiled in multi-year controversy in Europe and North America due to their risk to beneficial...
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Loss the contact closure signal
our 7600 couple with nanoLC Ultimate 3000 via contact closure. it has run without any loss connection during the batch. Just yesterday, the last injection keep equilibrating system until the LC finished the gradient run. We closed the software and power off LC and MS then started again but it did not help. The Dionex engineer also checked their LC and triggerring cable found both are ok.
Echo MS system maintenance operations
The Echo® MS system is a robust platform, and like any other platform, requires some routine maintenance on a regular basis. Here are 3 maintenance steps that can help you ensure consistent, reliable performance: Immediately before use or after switching carrier...
Breaking down the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready
Sensitivity and robustness carry different meanings in the world of mass spectrometry. Generally, sensitivity refers to an instrument’s ability to achieve lower limits of detection (LOD). Robustness, on the other hand, refers to an instrument’s ability to consistently...
The honey sting
As a consumer it’s hard for me not to feel inundated with claims that our food is “all-natural” or “chemical-free” or that we should buy certain “superfoods” for their health benefits. We read labels and trust that the product we are buying is what we are truly...
Innovation that’s blasting through limitations in explosive detection
Mass spectrometry’s important role in identifying explosives The need for rapid explosive detection is now an unfortunate reality. The remit is multifaceted. The first is for preventative purposes, to protect us from any threat to life. The second is in the...
A new generation of therapeutic modalities
There are over 7,000 genetic diseases that could potentially be cured using gene therapy. Rare metabolic diseases, autoimmune disorders, cardiovascular disease and cancers are some of the top disease classes that can be addressed with gene therapies. With over 1,000...
Enhancing Biologics with CESI-MS Characterization
Comprehensive characterization of a biologic requires analysis at both the intact and digest levels, but these analyses can be complex and cumbersome. For example, with conventional liquid chromatography separations, researchers are often left with limited information...
Full, partial and empty capsid ratios for AAV analysis: What’s the big deal?
For many of you working to develop gene therapy drugs, you know that the time to market the drug is critical. Because gene therapeutics cure diseases by targeting specific genes, it is a constant race to see who develops the drug first. Unlike other classes of drugs where multiple medications can be used to treat a disease, whoever is first to develop a gene therapy drug wins.
Accurate mass LC-MS/MS for PFAS analysis without needing a blockbuster budget
If you’ve been following our recent blogs, you’ve probably seen quite a bit on how per- and polyfluoroalkyl substances (PFAS) are shaking up both the food and environmental industry. Even if you’ve not been following our blogs, you’ve probably seen a lot of media...
A rising star in food allergen research: proteomics of shellfish allergen
It’s important to know what you’re eating, especially if you suffer from a food allergy.
About 220 million people worldwide live with a food allergy.1 These numbers, along with the complexity and severity of conditions, continue to rise. In America, there are about 32 million food allergy sufferers—5.6 million of those are children under the age of 18.2.2 That’s 1 out of every 13 children, or about 2 in every classroom. From a financial perspective, the cost of food allergy childcare for US families is up to $25 billion
Fast and Simultaneous Analysis of Ethanol Metabolites and Barbiturates
While most analytes in forensic applications analyze well with positive ionization, there are analytes that show better ionization efficiency with negative ionization, for example, acidic compounds. These analytes include ethanol metabolites such as ethyl glucuronide (ETG), ethyl sulfate (ETS), and the barbiturates such as amobarbital, butabarbital, butalbital, pentobarbital, phenobarbital, and secobarbital.
It’s a Point and Click World with the X500B QTOF System for Biologics Characterization
Did you know the X500B QTOF system makes point and click workflows for Biologics Characterization possible on your mass spectrometer? The newly-designed SCIEX OS software interface brings to life fluid navigation and ease of use so you can keep moving forward on your scientific discoveries. In fact, it’s so simple to learn and operate that you and your team can be up and running faster than you might expect.
PPCP Detection in Drinkwater is no Match for the QTRAP® 5500
SCIEX is no stranger to drinking water analysis, and the Environmental Compendium once again addresses the topic in an application note which covers LC-MS/MS with Fast Polarity Switching. Using the QTRAP® 5500, researchers were able to detect a large panel of Pharmaceuticals and Personal Care Products (PPCP’s) while performing fast positive/negative switching all from a single injection. Method details go the distance in this application note, as five experiments were called into action for comparison.
From Extraction to Cleanup—Measuring Mycotoxins from Cornmeal Products
When we look at emerging food trends for this coming year, you may be surprised to find that the US cornmeal market is making headlines as it is set to grow at a compound annual growth rate (CAGR) of two percent by 20201. This is the type of news that excites us, scientists, as it reminds us why we test for things like mycotoxins in cornmeal and the impact such analysis has on farmers and food manufacturers.
See How Easy It Can Be to Get Expert Results for Biologics Characterization
Learning a new mass spec system can be a daunting task. Aside from the opportunity costs of training new users, you might face the hassle of downtime, and the wait to get expert help when needed. The X500B QTOF system puts a new spin on biologics characterization workflows because it is so easy to learn and operate that you can be up and running much faster than you expect. Powerful new software tools dramatically streamline method development and data processing, to enable everyone in your lab to get expert results. It’s fast because it’s easy, even for new users.
Getting a Clean Match in Forensic Toxicology using LC-MS/MS
As a forensic scientist, what holds you back in the lab? It’s a question we often ask ourselves here at SCIEX, as product development depends on customer wants, needs, satisfaction, and ease of workflow. Ensuring evidence can withstand forensic scrutiny, for example, correlates with the integrity of testing procedures. Knowing this, how do you convince your staff to be confident in results, or convey technical data to a non-technical courtroom audience? If you have been left wondering how to get to the bottom of topics like these, check out the following toxicology toolkit. It’s a bundle of resources at your fingertips that includes a webinar led by Tania A. Saski Ph.D., Northwest Physician Laboratories, Bellevue, Using QTRAP® Technology to Provide Accurate Identification and Confirmation Beyond a Reasonable Doubt, and so much more
Looking to Quantify and Identify Pesticides in your Food Samples?
Visit our offices on any given day, and you are likely to discover researchers putting mass spectrometry to the test. The hum of the mass spectrometer is as common as conversations as scientists are tasked with developing methods that can be applied in real-world lab scenarios. In this case, André Schreiber SCIEX, Concord, Ontario, Canada, detailed, Comprehensive Quantitation and Identification of Pesticides in Food Samples Using the SCIEX UltraLC 100* and the SCIEX QTRAP® 4500 System.
Discover the New X500B QTOF System, the Simpler, Faster Path to Biologics Characterization Answers
Have you ever wished for a compact instrument that delivers expert-level answers to your most complex biotherapeutic characterization challenges faster and easier than what you are doing now? At SCIEX, we recognize that even expert users want easier ways to perform daily characterization tasks and get great results every time. That’s why we set out to develop the X500B QTOF system: a robust and reliable new instrument and software solution that reduces complexity and simplifies biologics characterization workflows so every scientist can get expert-level results
How to Achieve Higher Sensitivity with Hybrid Immunoaffinity LC-MS Assays
Protein-based biotherapeutics, including monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) are a growing component of pharmaceutical companies’ drug pipelines. The growth of ADCs in particular is due to their ability to selectivity target and deliver a potent molecule to a cancer cell based on a specific tumor marker. In order to support this growing class of new drug molecules, robust and reliable bioanalytical methods are required. While ligand binding assays (LBAs) like ELISA have been the most popular platform for biotherapeutic quantitation, bioanalytical scientists have been increasingly adopting hybrid LBA/LC-MS methods in this area.
The Application of Research Grade MetabolitePilot™ Software for the Determination of the Catabolic Peptide Products of Exenatide
The stability of peptide and protein biotherapeutics directly impacts their pharmacokinetic profile, efficacy, and safety. It is therefore essential to characterize the stability of a given bio-therapeutic including both in-vivo and in-vitro catabolism, thereby...
LC-MS/MS Screening of 64 New Psychoactive Substances Using Dried Blood Spots
There is a lot you can tell from a droplet of blood as it’s snapshot of what could be present in a body at any given moment. In the following application note, LC-MS/MS Screening of 64 New Psychoactive Substances Using Dried Blood Spots, researchers did just...
LC-MS/MS Analysis of Emerging Food Contaminants
Ever wish you had access to the most up to date application methods but don’t know where to find them? The Food and Beverage Compendium is your one-stop resource for research notes ranging from pesticides, allergens, and antibiotics to mycotoxins, vitamins, and...
Is Your Beverage Truly 100% Fruit Juice?
There is nothing like the flavor of fruit juice whether freshly squeezed or made from concentrate to clench your thirst, except when it’s not 100 percent juice after all. As the following tech note, “Authenticity Assessment of Fruit Juices using LC-MS/MS and...
Superbugs, Antibiotic Resistance, and the QTRAP® 6500+ System
‘Superbugs’, or bacteria that have developed antibiotic resistance as a result of adapting to the drugs used in their treatment, are dangerous infections that doctors struggle to stop from spreading. Even common infections such as urinary tract infections and...
It’s Time to Enhance Your Food Testing
Are you looking for ways to up the ante on your LC-MS/MS when it comes to food testing? Researchers here have developed a method for the analysis of approximately 400 pesticides in food samples, and their work is available for viewing in this year’s compendium.
Top Five Misconceptions about Mass Spectrometry
Do you work in a lab handling precious samples yet, hesitant to make the move to mass spectrometry? Many laboratories just like yours continue to conduct sample analysis using ELISA assays, PCR scans, and amino acid tests because of their effectiveness. These processes work, so why change? Well, these type of analytical experiments can report false positive and negative results. You have trained your staff, know the process, and fingers crossed, not too many user errors have compromised analysis.
Rapid Separation Method for Intact Monoclonal Antibodies (Mab) Merges Charge Variant, Impurity, and Glycoform Analyses into a Single Assay
Throughout all stages of development and manufacture, monoclonal antibodies (mAbs) exhibit a great deal of structural complexity. After translation and folding, proteins undergo post-translational modifications, as well as spontaneous and enzymatic degradation, such that a single preparation of purified mAb exhibits a range of small structural changes, composed of various glycoforms and charge variants, as well as amino acids alterations due to oxidation, deamidation, isomerization, or other chemical reactions. This display of structural heterogeneity can influence the overall stability, efficacy, and safety profile; therefore, understanding the extent of structural modifications has become extremely important to drug manufacturers who continually assess mAb composition throughout bioprocessing to demonstrate stability, batch-to-batch consistency, and long-term shelf life.
Glycosylation Analysis Designed for the (Protein) Masses
A variety of post-translational modifications (PTMs) can impact a biotherapeutic protein’s mass, but none are as common as glycosylation.[1] Hence, the headline for a recent article in Genetic Engineering and Biotechnology News, “Post-Translational Icing on the Biologics Cake,” featuring comments from Sean McCarthy, Ph.D., Global Market Manager of Biologics at SCIEX.
Struggling to Analyze Small Volume Samples with Conventional LC-MS?
The M3 MicroLC System is designed for scientists who are struggling to analyze small volume samples with conventional LC-MS and need to lower their limits of quantitation while maintaining throughput and robustness. When designing the M3 MicroLC System, we...
Legal and Illicit Drugs in Wastewater Detected and Confirmed with QTRAP Technology
What happens when you up the sensitivity and lower detection limits on influent and effluent sewage tests? For starters, low levels of illegal drugs in samples begin to emerge. This is what researchers discovered when they combined the power of LC-MS/MS with the...