Bulletproof your clinical laboratory with rugged and reliable LC-MS/MS instrumentation (Clinical diagnostic blog) by Pierre Negri | 0 CommentsThe ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
Bulletproof your clinical laboratory with rugged and reliable LC-MS/MS instrumentation (Clinical research blog) by Pierre Negri | 0 CommentsThe ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
Methods for electrode cleaning for Echo MS systems by John Gibbons | 0 CommentsDepending on the samples that you are running on the system, it is possible for the Echo MS electrode to become dirty or occluded over time. Below are two different cleaning strategies that will be helpful for you to maintain your system and keep your electrodes running well.
The myth of costly training: debunked. by Andrea Mueller | 0 CommentsAs analytical organizations grow, there is an even greater need to train scientists and operators more consistently to meet tight deadlines, handle increasing samples, and meet data quality expectations. A high rate of employee turnover also affects the productivity of labs worldwide. Consistent training helps today’s labs stay competitive, whether the goal is sample throughput, therapeutic development, or publication.
Pumpkin spice season: A deep dive into spice authenticity by Gitte Barknowitz | Blogs, Food / BeverageThanks to Starbucks, who launched the pumpkin spice latte in 2003 (yes, over 20 years ago), the spice mixture became a global phenomenon, loved and disliked at the same time.
Celebrating customer experience: Insights from SCIEX leaders by Andrea Mueller | Biopharma, Blogs, Clinical, Environmental / Industrial, Food / Beverage, Forensic, Life Science Research, Pharma, TechnologyCustomer Experience Day (CX Day) is a special occasion for SCIEX, celebrated every first Tuesday in October. It’s a day dedicated to recognizing the incredible value of our customers and the relentless dedication of our associates who strive to make every interaction meaningful. At SCIEX, our commitment to customer experience is woven into the fabric of everything we do. From providing cutting-edge solutions to offering unwavering support, we aim to build lasting relationships and exceed expectations. Join us as we delve into the insights from our leaders on what customer experience means to SCIEX and how it shapes our journey.
How is EAD transforming from a niche technique into a cornerstone of daily metabolomics and lipidomics research? by Rebekah Sayers | Blogs, Life Science ResearchWith the launch of the ZenoTOF 8600 system, EAD has taken a significant leap forward in becoming a routine tool for metabolomics and lipidomics workflows. Building on the foundation laid by the ZenoTOF 7600 system, the 8600 system introduces enhanced sensitivity, function speed improvements, and multimodal capabilities that make EAD more practical and scalable for daily use. This blog explores how these advancements are transforming EAD from a specialized technique into a robust and accessible solution for high-throughput structural analysis, enabling researchers to unlock deeper insights with greater efficiency.
Q/A: Quantitative and qualitative solutions for large-panel pesticides assays using SCIEX Triple Quad and QTRAP systems by Holly Lee | Blogs, Food / Beverage, QTRAP / Triple QuadIn a recent webinar, which is now available on-demand, Holly Lee powerful strategies to tackle complex residue testing. From boosting throughput to fine-tuning method sensitivity, Holly shared key ways to maximize performance across large pesticide panels.
Bulletproof your clinical laboratory with rugged and reliable LC-MS/MS instrumentation (Clinical diagnostic blog) by Pierre Negri | Blogs, Clinical, Ion sourcesThe ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
Bulletproof your clinical laboratory with rugged and reliable LC-MS/MS instrumentation (Clinical research blog) by Pierre Negri | Blogs, Clinical, Ion sourcesThe ability to consistently achieve reproducible results on many complex samples across multiple days is critical to a routine clinical laboratory. Laboratories relying on analytical instrumentation require stability and robustness to perform a variety of screening and confirmatory assays with confidence. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the preferred analytical method in the clinical laboratory to reliably perform clinical testing as it provides best-in-class performance and reliability for the most challenging assays. LC-MS/MS offers the required levels of sensitivity and specificity for the detection and quantitation of molecules from complex biological samples, helping laboratories deliver highly accurate data for a variety of clinically relevant analytes across a wide range of assays.
Methods for electrode cleaning for Echo MS systems by John Gibbons | Blogs, Echo® MS+ system, PharmaDepending on the samples that you are running on the system, it is possible for the Echo MS electrode to become dirty or occluded over time. Below are two different cleaning strategies that will be helpful for you to maintain your system and keep your electrodes running well.
The myth of costly training: debunked. by Andrea Mueller | Blogs, Instrument maintenance, Optimizing Performance, Technology, TrainingAs analytical organizations grow, there is an even greater need to train scientists and operators more consistently to meet tight deadlines, handle increasing samples, and meet data quality expectations. A high rate of employee turnover also affects the productivity of labs worldwide. Consistent training helps today’s labs stay competitive, whether the goal is sample throughput, therapeutic development, or publication.
Tackling mycotoxin testing: Harnessing mass spectrometry for safer foods and healthier futures by SCIEX Community | Blogs, Food / Beverage, Food and BeverageProduced by certain moulds, thriving in crops such as grain, nuts and coffee, mycotoxins have contaminated agriculture and food production industries for a long time. To intensify the challenge, mycotoxins are resilient, not easily broken down and ensuring the safety of food supply chains requires comprehensive solutions and we are here to share those solutions with you.
Enhancing omics-based mass spectrometry workflows with Electron-Activated Dissociation (EAD) by Rebekah Sayers | Blogs, Life Science ResearchElectron-Activated Dissociation (EAD) is transforming the fields of metabolomics and lipidomics by providing enhanced fragmentation techniques that offer deeper insights into molecular structures. In September, Technology Networks hosted a webinar, “Enhancing Mass-Based Omics Analysis in Model Organisms,” featuring Dr. Valentina Calabrese from the Institute of Analytical Sciences at the University of Lyon. Valentina shared her insights on improving omics-based mass spectrometry analysis for toxicology studies using model organisms, particularly in metabolomics and lipidomics. This blog explores the additional functionalities EAD offers, its benefits in untargeted workflows, its incorporation into GNPS and molecular networking, and the future role it could play in these scientific domains.
Tips and tricks from our application experts: AAV analysis with CE by Peter Holper | Biopharma, BioPhase 8800 system, Blogs, PA 800 systemPeter Holper, Staff Applications Scientist at SCIEX, US, shares his tips and tricks on AAV analysis using CE with the BioPhase 8800 system and the PA 800 Plus system.
Overcoming uncertainty in your PFAS analysis by Karl Oetjen | Blogs, Environmental / IndustrialJust like gum on the bottom of a shoe, the existence of per- and poly-fluorinated alkyl substances (PFAS) in our environment is a sticky one. If you’re in the field of environmental testing, then you’re all too familiar with the threat these substances have on public health. While we have learned a lot about them over the years, there is still much more to understand. With the right detection methods, we can gather the information we need to empower us to make informed decisions on reducing the risks they impose.
High mass tuning calibration for ZenoTOF 7600 by Gregg Czerwieniec | Bio Tool Kit, Development, Questions, ZenoTOF 7600 systemHi,
Metabolite identification and peace of mind by Kirsten Craven | Blogs, LC, Pharma, QTRAP / Triple Quad, ZenoTOF 7600 systemManaging metabolite identification (Met ID) studies is challenging, so what is at the top of your priority list as you plan the year ahead? Ensuring you have the data needed to manage product safety, meeting deadlines, staff recruitment and training, maintaining compliance, capital expenses, or something else?
Optimized rolling collision energy curves for IDA and SWATH DIA for peptides by Christie Hunter | Data acquisition, Life Science Research, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemDuring data dependent acquisition (DDA or IDA) or SWATH acquisition, the collision energy can be automatically adjusted according to the mass/charge and charge of the peptide. This dependency has been well characterized on our QTOF systems. By selecting rolling...
Current proteomics software compatibility for ZenoTOF 7600 system by Christie Hunter | Data processing, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemBelow is a summary of various other software packages that are useful for processing proteomics data from the ZenoTOF 7600 system. Note this list is not comprehensive and only covers the tools we have lightly tested to date. Acquisition Type Software Files needed...
Processing ZenoTOF 7600 system data with DIA-NN software by Christie Hunter | Data processing, Life Science Research, Proteomics, SCIEX information, ZenoTOF 7600 systemDIA-NN software is a powerful software tool for processing data independent acquisition (DIA) proteomics datasets. It uses neural networks and other algorithms to identify and quantify peptides and proteins from DIA data and is specifically optimized for fast...
The hidden ingredient in anti-fog sprays: PFAS by Craig Butt | Agriculture, Blogs, Environmental / Industrial, IndustrialA recent study led by researchers from Duke University, conducted with colleagues from Wayne State University and the University of North Carolina at Charlotte, found that four of the top-rated anti-fog sprays contained up to 20.7 milligrams of per- and polyfluoroalkyl substances (PFAS) per milliliter of solution. This study has received a lot of exposure in popular media, and it should not be overlooked. The researchers used several creative approaches to obtain a comprehensive characterization of the anti-fog sprays, including using total organic fluorine measurements, GC-MS methods and both nominal mass and accurate mass instruments. A unique finding of the study was the detection of fluorotelomer ethoxylates (FTEOs), which are relatively unknown PFAS compounds.
Excel macro for calculating averages or percent CVs from replicates in a 384 -well plate by Mackenzie Pearson | Bioanalysis/PK, Data processing, Data Visualization, Echo® MS+ system, Pharma, SCIEX information, SCIEX OS softwareThis excel workbook is designed to be used with data copied from a SCIEX OS software Analytics module results file
Back to the new basics: Part 1 | Making the leap from GC-MS to LC-MS by Alex Liu | Biopharma, Blogs, Clinical, Environmental / Industrial, Food / Beverage, Forensic, Life Science Research, PharmaProducing accurate results quickly in a demanding environment is no easy feat for analytical scientists. What’s more, many of us are constantly questioning ourselves—I certainly am—about whether we are employing the best technique for the analysis at hand. It’s an overwhelming thought, considering the wide range of tools that are available to choose from, each of which offers varying levels of capacity, sensitivity, selectivity, specificity and cost. How do you meet the unique needs of your organization without breaking the bank? I get it, and I’m not here to convince you it’s easy. My aim is to guide you through the process to help you make the right decision for you.
How do I check the quality of the Auto Retention Time Calibration used in my Extractor processing? by Christie Hunter | Data Visualization, Life Science Research, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemWhen using the Auto-Calibration option in Extractor, a set of retention time calibration peptides will be determined automatically from the library and used for RT calibration. To determine how the fit looks for the calibration on each datafile, follow these steps....
Uploading data for use in OneOmics suite by Alexandra Antonoplis | Data processing, Life Science Research, Metabolomics, Multi-Omics, OneOmics, Proteomics, QTOF, SCIEX information, ZenoTOF 7600 systemThere are two options for data storage when working with the OneOmics suite. You can store your data either within the SCIEX Cloud platform in the Data Store, or you can store data in BaseSpace (Illumina) and link your BaseSpace account with the SCIEX Cloud platform....
sMRM Pro Builder template tutorial by Mackenzie Pearson | Data processing, Data Visualization, How-to videos, Life Science Research, Lipidomics, Metabolomics, Optimizing Performance, QTRAP / Triple Quad, ResultsThe sMRM Pro Builder template is an Excel-based tool that can help you implement large panels of analytes in your lab. The Excel sheet will take your preliminary experimental results and compute retention times, retention time window widths and dwell time weighting to optimize your targeted assay.
Short-chain PFAS compounds are on the rise- Craig’s PFAS Vodcast Cora Young by Craig Butt | Blogs, Environmental / IndustrialRead time: 2 minutes Short-chain per- and polyfluoroalkyl substances (PFAS) are increasing in the Canadian Arctic environment, with the most rapid increases occurring post-2000, according to a recent study in Geophysical Research Letters (April 2020). For example,...
Thailand cannabis legalization by Diana Tran | BlogsThailand has become the first southeast Asian country to legalize cannabis for medical use. Cannabis was originally introduced into Thailand from India, and until it was outlawed in the 1930s, it was historically used as a kitchen condiment, medicine and source of fiber.
Reducing PFAS interferences during human plasma and serum analysis with accurate mass spectrometry by Ella Fono | Application / Technical Notes, Featured Content
High level method optimization considerations for Echo MS system by Mackenzie Pearson | Biopharma, Data acquisition, Echo® MS+ system, Environmental / Industrial, Food / Beverage, Life Science Research, Optimizing Performance, Pharma, SCIEX information, TechnologyWhile an in-depth discussion of method development and optimization for the Echo® MS system is beyond the scope of a community post, here are some points to consider as part of the process: The maximum recommended ion spray voltage for prolonged electrode life is 5000...
High complexity of the lipidome by Mackenzie Pearson | Data acquisition, Food / Beverage, How-to videos, Life Science Research, Lipidomics, ZenoTOF 7600 systemThe complexity of the lipidome is diverse in the structure and there are many combinatorial isoforms that are available within nature. Currently, many different techniques are required to fully characterize a lipid molecule. What if you could do it in a single...
Standard front-end cleaning for TripleTOF systems by Christie Hunter | Instrument maintenance, QTOF, SCIEX information, TechnologyGeneral points: Use of laboratory deionized (DI) water is specified at points in the procedure. However, if there are any concerns regarding quality, please utilize a reputable supplier of pure deionized water (>18.2M ohm). 5-10 liters will typically be required...
Stay informed on the latest novel psychoactive substances (NPS) flooding the US drug market by Pierre Negri | Forensic, QTOF, QTRAP / Triple Quad, Results, SCIEX information, Toxicology, ZenoTOF 7600 systemEach year, the Drug Enforcement Administration (DEA) Special Testing and Research Laboratory publishes an Emerging Threat Report. The data contained in the report is updated quarterly and represents a snapshot of the drug evidence seized and analyzed by the DEA in the...